Effect of progesterone on Candida albicans biofilm formation under acidic conditions: A transcriptomic analysis

Gonçalves, Bruna; Bernardo, Ruben; Wang, Can; Schröder, Markus; Pedro, Nuno A.; Butler, Geraldine; Azeredo, Joana; Henriques, Mariana; Mira, Nuno P.; Silva, Sónia

doi:10.1016/j.ijmm.2020.151414

Cited by 11 publications

(2 citation statements)

References 48 publications

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“…Therefore, a safe concentration for eradicating C. albicans biofilms has been determined at 0.45 mg mL –1 of the OEO-KMPs. Fluconazole, used as a reference point, revealed an MIC of 0.25 μg mL –1 when exposed to C. albicans SC5314 biofilm cells at pH 4, similar to vaginal fluid . However, recent studies have revealed greater resistance of this species to fluconazole .…”

Section: Resultsmentioning

confidence: 93%

Development and Evaluation of Microencapsulated Oregano Essential Oil as an Alternative Treatment for Candida albicans Infections

Fernandes,

Barco-Tejada,

Blázquez

et al. 2024

ACS Appl. Mater. Interfaces

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Vulvovaginal candidiasis (VVC) is characterized as a very common fungal infection that significantly affects women’s health worldwide. Essential oils (EOs) are currently being evaluated as an alternative therapy. The development of efficient techniques such as micro- or nanoencapsulation for protecting and controlling release is essential to overcome the limitations of EO applications. Therefore, the aim of this study was to develop and characterize oregano EO-loaded keratin microparticles (OEO-KMPs) as a potential treatment for VVC. OEO-KMPs were produced using high-intensity ultrasonic cycles and characterized in terms of morphological and physicochemical parameters. In vitro evaluation included assessing the toxicity of the OEO-KMPs and their effect against Candida albicans using microdilution and agar diffusion, while the activity against biofilm was quantified using colony forming units (CFU). The efficacy of the OEO-KMPs in an in vivo VVC mouse model was also studied. Female BALB/c mice were intravaginally infected with C. albicans, 24 h postinfection animals were treated intravaginally with 15 μL of OEO-KMPs and 24 h later vaginal fluid was analyzed for C. albicans and Lactobacillus growth (CFU mL–1). The results showed the stability of the OEO-KMPs over time, with high encapsulation efficiency and controlled release. This nanoparticle size facilitated penetration and completely inhibited the planktonic growth of C. albicans. In addition, an in vitro application of 2.5% of the OEO-KMPs eradicated mature C. albicans biofilms while preserving Lactobacillus species. In in vivo, a single intravaginal application of OEO-KMPs induced a reduction in C. albicans growth, while maintaining Lactobacillus species. In conclusion, this therapeutic approach with OEO-KMPs is promising as a potential alternative or complementary therapy for VVC while preserving vaginal microflora.

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Section: Resultsmentioning

confidence: 93%

Development and Evaluation of Microencapsulated Oregano Essential Oil as an Alternative Treatment for Candida albicans Infections

Fernandes,

Barco-Tejada,

Blázquez

et al. 2024

ACS Appl. Mater. Interfaces

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“…SPT23 regulates lipid desaturation and ergosterol biosynthesis, playing an important role in the ability of yeast cells to adapt to environmental changes ( 42 , 43 ). SFL1 is involved in acidic biofilm formation and hyphal development in the pathogenic yeast Candida albicans ( 44 , 45 ) and in determining virulence and heat tolerance in the phytopathogenic filamentous fungus Magnaporthe oryzae ( 46 ). To date, there have been no reports of the effects of SPT23 and SFL1 on the expression of cellulase and xylanase genes in fungi, and further study is therefore needed.…”

Section: Resultsmentioning

confidence: 99%

Three-Dimensional Genome Map of the Filamentous Fungus Penicillium oxalicum

Liu²,

Zhang

et al. 2022

Microbiol Spectr

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The spatial structure of chromatin plays important roles in normal cell functions and the regulation of gene expression. The three-dimensional (3D) architectures of the genomes of many mammals and plants have been elucidated, but corresponding studies on filamentous fungi, which play vital roles as decomposers of organic matter in the soil, are very limited. Penicillium oxalicum is one of the predominant cellulolytic aerobic fungi in subtropical and tropical forest soils and can secrete integrative cellulase and xylanase under integrated regulatory control, degrading plant biomass highly efficiently.

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Clinical Significance, Molecular Formation, and Natural Antibiofilm Agents of Candida albicans

Abdulghani,

Zore

2024

Advances in Antifungal Drug Development

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Effect of progesterone on Candida albicans biofilm formation under acidic conditions: A transcriptomic analysis

Cited by 11 publications

References 48 publications

Development and Evaluation of Microencapsulated Oregano Essential Oil as an Alternative Treatment for Candida albicans Infections

Development and Evaluation of Microencapsulated Oregano Essential Oil as an Alternative Treatment for Candida albicans Infections

Three-Dimensional Genome Map of the Filamentous Fungus Penicillium oxalicum

Clinical Significance, Molecular Formation, and Natural Antibiofilm Agents of Candida albicans

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