Effect of Progesterone Induced Increase in Uterine Secretory Activity on Development of the Porcine Conceptus

Knight, James; Bazer, Fuller W.; Wallace, H. D.

doi:10.2527/jas1974.394743x

Cited by 32 publications

(21 citation statements)

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“…By mid-pregnancy, the lumens of uterine glands are greatly enlarged and filled with secretory products (Perry & Crombie 1982, Sinowatz & Friess 1983. During early pregnancy, the pig uterus secretes only moderate levels of histotroph, but total uterine secretions increase dramatically after day 30 of pregnancy (Knight et al 1974, Schlosnagle et al 1974, Basha et al 1980. Although progress has been made toward deciphering the mechanism(s) through which P 4 influences endometrial GE development in other species, these processes are poorly understood in pigs.…”

Section: Discussionmentioning

confidence: 99%

“…The second involves 10-15% of the remaining conceptuses and occurs during early to midgestation (Pope 1994). This second period of conceptus loss correlates with increasing GE development, endometrial secretory activity, placental growth and differentiation, and fetal growth (Knight et al 1974, 1977, Perry & Crombie 1982, Sinowatz & Friess 1983, Dwyer et al 1994. Much is not understood about the mechanisms that underlie these waves of conceptus loss.…”

Section: Discussionmentioning

confidence: 99%

“…P 4 stimulates transformation of the endometrium into a secretory tissue to create an environment permissive to early embryonic development, implantation, placentation, and fetal/placental development (Spencer et al 2004). Importantly, P 4 increases the expression of various uterine secretory proteins that contribute to histotroph, which supports conceptus development in pigs (Knight et al 1974, Roberts & Bazer 1988. The majority of histotroph is thought to be secreted by the endometrial glandular epithelium (GE).…”

Section: Introductionmentioning

confidence: 99%

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Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

et al. 2010

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Pigs experience significant conceptus loss near mid-gestation, correlating with increasing glandular epithelial (GE) development and secretory activity. Secreted phosphoprotein 1 (SPP1, osteopontin) increases in GE between days 30 and 40 of pregnancy and is expressed in the GE of day 90 pseudopregnant pigs, suggesting that progesterone (P 4 ) from corpora lutea is responsible for induction of SPP1 in GE. In this study, pigs were ovariectomized and treated daily with P 4 to assess effects of 40 days of P 4 exposure on SPP1, P 4 receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 (FGF7) expression in porcine endometria. PGR mRNA decreased in pigs injected with P 4 compared with pigs injected with corn oil (CO), and PGRs were downregulated in the luminal epithelium (LE) and GE. ACP5 mRNA increased in pigs injected with P 4 compared with pigs injected with CO, and ACP5 was induced in the GE of P 4 -treated pigs. FGF7 mRNA increased in pigs injected with P 4 compared with pigs injected with CO, and FGF7 was induced in the LE and GE of P 4 -treated pigs. SPP1 mRNA was not different between pigs injected with P 4 compared with pigs injected with CO, and SPP1 was not present in the GE of P 4 -treated pigs. Therefore, long-term P 4 , in the absence of ovarian and/or conceptus factors, does not induce SPP1 expression in GE. We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

et al. 2010

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“…Although similar gene expression studies have not been performed in pigs, progesterone increases the expression of calbindin-D9k (Yun et al 2004), vascular endothelial growth factor (Welter et al 2004), fibroblast growth factors (FGFs) 2 and 7, two FGF receptors, FGFR1 and FGFR2 (Welter et al 2003, a 4 , a 5 , and b 1 integrin receptor subunits (Bowen et al 1996), the swine leukocyte antigens 1, 2, and 3, and b(2)-microglobulin (Joyce et al 2008), as well as suppresses expression of MUC1 and progesterone receptor in luminal epithelium (LE; Geisert et al 1994, Bowen et al 1996. Importantly, progesterone increases the expression of various uterine secretory proteins that are components of the histotroph, which is hypothesized to support conceptus and fetal development in pigs (Knight et al 1974a, 1974b, Roberts & Bazer 1988.…”

Section: Introductionmentioning

confidence: 99%

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Bailey¹,

Dunlap²,

Frank³

et al. 2010

Reproduction

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In pigs, endometrial functions are regulated primarily by progesterone and placental factors including estrogen. Progesterone levels are high throughout pregnancy to stimulate and maintain secretion of histotroph from uterine epithelia necessary for growth, implantation, placentation, and development of the conceptus (embryo and its extra-embryonic membranes). This study determined effects of longterm progesterone on development and histoarchitecture of endometrial luminal epithelium (LE), glandular epithelium (GE), and vasculature in pigs. Pigs were ovariectomized during diestrus (day 12), and then received daily injections of either corn oil or progesterone for 28 days. Prolonged progesterone treatment resulted in increased weight and length of the uterine horns, and thickness of the endometrium and myometrium. Hyperplasia and hypertrophy of GE were not evident, but LE cell height increased, suggesting elevated secretory activity. Although GE development was deficient, progesterone supported increased endometrial angiogenesis comparable to that of pregnancy. Progesterone also supported alterations to the apical and basolateral domains of LE and GE. Dolichos biflorus agglutinin lectin binding and a v integrin were downregulated at the apical surfaces of LE and GE. Claudin-4, a 2 b 1 integrin, and vimentin were increased at basolateral surfaces, whereas occludins-1 and -2, claudin-3, and E-cadherin were unaffected by progesterone treatment indicating structurally competent trans-epithelial adhesion and tight junctional complexes. Collectively, the results suggest that progesterone affects LE, GE, and vascular development and histoarchitecture, but in the absence of ovarian or placental factors, it does not support development of GE comparable to pregnancy. Furthermore, LE and vascular development are highly responsive to the effects of progesterone.

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“…Recently, the biochemistry of uterine fluids of swine and the relationship to the incidence of embryonic mortality has been studied (Murray, Bazer, Wallace & Warnick, 1972;Knight, Bazer & Wallace, 1974). So far, however, collection of uterine fluid has not been achieved consecutively in the same animal without some form of chronic cannulation (Iritani, Sato & Nishikawa, 1974).…”

Section: (Received 17th December 1974)mentioning

confidence: 99%

Laparoscopic Pregnancy Diagnosis and Uterine Fluid Recovery in Swine

Wildt¹,

Morcom²,

Dukelow³

1975

Reproduction

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Effect of Progesterone Induced Increase in Uterine Secretory Activity on Development of the Porcine Conceptus

Cited by 32 publications

References 0 publications

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Laparoscopic Pregnancy Diagnosis and Uterine Fluid Recovery in Swine

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