“Effect of process variables on solubility and recovery yields of proteins from pangas (Pangasius pangasius) frames obtained by alkaline solubilization method: Characteristics of isolates”

Surasani, Vijay Kumar Reddy; Khatkar, Sunil Kumar; Singh, Sandeep

doi:10.1016/j.fbp.2017.09.008

Cited by 29 publications

(27 citation statements)

References 30 publications

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“…Isolates extracted using the acid process had low FC and EC (11.66 and 58.33%) compared to alkali processed isolates (48.33 and 78.33%) ( P < 0.05). The differences in FC and EC are attributed to the amount of hydrophobic residues in the proteins . Acid and alkali extracted proteins from freshwater mussel had FC values of 50.93 and 43.48%, respectively .…”

Section: Resultsmentioning

confidence: 99%

“…The differences in FC and EC are attributed to the amount of hydrophobic residues in the proteins. 8 Acid and alkali extracted proteins from freshwater mussel had FC values of 50.93 and 43.48%, respectively. 2 Pangas protein isolates extracted using acid and alkali processes had FC values of 66.29 and 54.50%, respectively.…”

Section: Water Holding Capacity Oil Holding Capacity Foaming and Emmentioning

confidence: 97%

“…Homogenate was adjusted to pH 3.0 and pH 11.0 with glacial acetic acid and NaOH (10 mol L −1 ) with gentle stirring. 8 The pH was monitored continuously with an electrode attached to the digital pH meter (Systronics India Ltd, Gujarat, India). The pH of the homogenate was kept constant throughout the process by adding dilute acid and alkali whenever needed.…”

Section: Protein Isolation Solubilizationmentioning

confidence: 99%

“…With this aim, a methodology called pH shift process was developed by Hultin and Kelleher that enabled the effective protein recovery from underutilized by‐products and processing waste. Thereafter, many studies have been conducted on protein recovery from low‐cost fish, fish by‐products and processing waste through a pH shift process, to obtain their characterization and application . Almost all the studies used hydrochloric acid (HCl) and sodium hydroxide (NaOH) for protein solubilization during acid‐ and alkali‐aided extraction processes.…”

Section: Introductionmentioning

confidence: 99%

“…Thereafter, many studies have been conducted on protein recovery from low-cost fish, fish by-products and processing waste through a pH shift process, to obtain their characterization and application. [6][7][8][9][10] Almost all the studies used hydrochloric acid (HCl) and sodium hydroxide (NaOH) for protein solubilization during acid-and alkali-aided extraction processes. Many studies reported that proteins recovered using HCl in acid-aided solubilization showed greater denaturation and associated loss in functionality over alkali-aided solubilization.…”

Section: Introductionmentioning

confidence: 99%

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Effect of organic acid on recovery yields and characteristics of rohu (Labeo rohita) protein isolates extracted using pH shift processing

et al. 2019

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BACKGROUND Proteins recovery using hydrochloric acid (HCl) in acid‐aided solubilization could cause greater loss in its functionality over alkali‐aided solubilization. Moreover, using HCl in edible foods is also a health concern. Replacing HCl with organic acids for acid‐aided solubilization could address these problems. The aim was to study the effect of organic acid (glacial acetic acid) as a replacement for HCl during pH shift processing on the characteristics and functionality of rohu (Labeo rohita) protein isolates. Rohu proteins were obtained by solubilizing at pH 3.0 and pH 11.0 using glacial acetic acid and sodium hydroxide (10 mol L−1). RESULTS Results showed that solubilization at pH 11.0 gave higher protein yields (766.8 ± 2.4 g kg−1) compared to solubilization at pH 3.0 (735.7 ± 7.1 g kg−1) (P < 0.05). Isolates from acid‐aided solubilization had higher whiteness and total pigment content over isolates obtained by alkali‐aided solubilization. Rohu isolates recovered by alkaline solubilization showed higher water and oil holding capacity, gel strength, folding scores, foaming and emulsion capacity than acid processed isolates (P < 0.05). Solubilization of rohu proteins using glacial acetic acid produced isolates with low breaking force (149.0 g), low storage modulus (G′) values and low folding test score (1.0) over the alkaline isolates (P < 0.05). CONCLUSION Results indicated that, recovering rohu proteins using organic acid (glacial acetic acid) could produce isolates with poor functional properties, while using the organic acid to precipitate the proteins solubilized by alkali‐aided processing could produce proteins with better yields and functionality. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Water Holding Capacity Oil Holding Capacity Foaming and Emmentioning

confidence: 97%

Section: Protein Isolation Solubilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of organic acid on recovery yields and characteristics of rohu (Labeo rohita) protein isolates extracted using pH shift processing

et al. 2019

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Biorefinery of Underutilized Marine Resources Using the pH‐Shift Technology

ABDOLLAHI

2023

Current Challenges for the Aquatic Products Processing Industry

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Optimisation of alkaline extraction of protein from brewer’s spent grain

Karlsen

Lund

Skov

2022

J Institute Brewing

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Extraction of proteins from industrial residues is a potential source for animal feed. Alkaline extraction combined with isoelectric precipitation may be a useful method for isolating protein from brewer's spent grain (BSG), an abundant by-product from the brewing industry. The objective of this study was to examine the effect of temperature (30, 45 and 60°C), time (30, 60, 120 and 180 minutes), agitation (shaking and stirring), precipitation salinity (0.5 M sodium chloride), isolation mode (freeze drying and precipitation), pre-treatment (defatting and delignification) and repeated extraction on the protein yield and protein content from BSG. Generally, the protein content decreased while protein yield increased with increasing extraction temperatures. Yield and content were maximised after 30 minutes reaching the highest protein content at 30 o C (54.8%) and the highest protein yield at 60 o C (10.5%). Precipitation with 0.5 M sodium chloride reduced the protein content to 36.4% and the yield to 4.2%. Freeze drying combined with triple extraction yielded a protein content of 20-25%, together with a relatively high protein yield (ca. 45- 50%). Delignification increased the protein content from 27% to 32% whereas defatting reduced the overall protein yield from 45% to 38%. Combining isoelectric precipitation and single extraction was associated with a higher protein content (40-55%) but poor protein yields (5-10%). In the light of these findings, further research is required to establish conditions that maximise both the protein content and yield.

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“Effect of process variables on solubility and recovery yields of proteins from pangas (Pangasius pangasius) frames obtained by alkaline solubilization method: Characteristics of isolates”

Cited by 29 publications

References 30 publications

Effect of organic acid on recovery yields and characteristics of rohu (Labeo rohita) protein isolates extracted using pH shift processing

Effect of organic acid on recovery yields and characteristics of rohu (Labeo rohita) protein isolates extracted using pH shift processing

Biorefinery of Underutilized Marine Resources Using the pH‐Shift Technology

Optimisation of alkaline extraction of protein from brewer’s spent grain

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