Effect of Process Factors on the Properties of Doxycycline Nanovesicles

Honary, Soheila; Zahir, Foruhe

doi:10.4314/tjpr.v11i2.1

Cited by 59 publications

(54 citation statements)

References 16 publications

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“…According to the NPs characterization results, positively charged spherical NPs with diameters <200 nm were obtained and the particle sizes showed a component concentration‐dependent manner. Honary et al . indicated that the increased adsorption of positively charged NPs to cancer cells which can be explained by negative‐charged cell surfaces due to abundance of phospholipids and proteins.…”

Section: Discussionmentioning

confidence: 99%

Docetaxel–Chitosan nanoparticles for breast cancer treatment: cell viability and gene expression study

et al. 2016

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Docetaxel acts through the inhibition of tubulin polymerization and reduction in the expression of BCL-2 gene. In this study, nanoparticles containing Docetaxel were prepared and their effects on the gene expression levels of BCL-2 and BAX genes were investigated. The drug was first conjugated to chitosan, and the nanoparticles were assembled in the presence of hyaluronic acid. Conjugations were confirmed by H-NMR, and the obtained nanoparticles were characterized by dynamic light scattering and SEM. Cytotoxicity of the nanoparticles, cellular uptake, and cell death were evaluated. Finally, the effect of nanoparticles on the expression of BAX and BCL-2 genes in MCF-7 cells were investigated through real-time PCR. The results revealed that the prepared NPs had spherical shape with narrow size distribution of<200 nm with positive zeta potentials. In vitro cytotoxicity of Cs nanoparticles and free Docetaxel investigations revealed that increasing the treatment time with nanoparticles led to decrease in the rate of cell viability. BAX and BCL-2 gene expressions were decreased in nanoparticle-treated cells in comparison with intact cells, while the BAX/BCL-2 ratio was significantly elevated compared with free drug-treated cells after 72 h. Docetaxel-conjugated NPs may offer a promising treatment with low off-target toxicity for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Docetaxel–Chitosan nanoparticles for breast cancer treatment: cell viability and gene expression study

et al. 2016

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“…As the zeta potential becomes more negative, we hypothesize that this decrease is highly likely attributed to the rearrangement of siRNA on the surface of the complexes, due to the friction or pressure applied during nebulization. However, it is important to notice that the complexes are still positively charged after nebulization, enabling interaction with the negatively charged cell membrane …”

Section: Resultsmentioning

confidence: 99%

High‐Pressure Nebulization as Application Route for the Peritoneal Administration of siRNA Complexes

Minnaert

Dakwar

Benito

et al. 2017

Macromolecular Bioscience

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Peritoneal carcinomatosis is a severe form of cancer in the abdomen, currently treated with cytoreductive surgery and intravenous chemotherapy. Recently, nebulization has been proposed as a less invasive strategy for the local delivery of chemotherapeutic drugs. Also, RNA interference has been considered as a potential therapeutic approach for treatment of cancer. In this study, Lipofectamine RNAiMAX/siRNA complexes and cyclodextrin/siRNA complexes are evaluated before and after nebulization. Nebulization of the siRNA complexes does not significantly lower transfection efficiency when compared to non‐nebulized complexes. After incubation in ascites fluid, however, the cyclodextrin/siRNA complexes show a drastic decrease in transfection efficiency. For the Lipofectamine RNAiMAX/siRNA complexes, this decrease is less pronounced. It is concluded that nebulization is an interesting technique to distribute siRNA complexes into the peritoneal cavity, providing the complexes are stable in ascites fluid which might be present in the peritoneal cavity.

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“…A zeta potential of more than ±5 mV indicated that particles were safe from rapid coagulation. However, since the value is within ±30 mV, the suspension cannot be considered stable . Owing to this property, it was easy to separate the particles by centrifugation.…”

Section: Resultsmentioning

confidence: 99%

Magnetic particles for sugar separation from sulphuric acid solution generated during nano‐crystalline cellulose production

et al. 2017

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Nano-crystalline cellulose (NCC) is a renewable material having different applications ranging from drug delivery to a reinforcing filling agent in polymer synthesis. Concentrated sulphuric acid is used to hydrolyze cellulosic biomass to obtain NCC. Manufacturers are keen to reuse the diluted acid solution left after the process. However, the presence of mono and oligosaccharides makes it unsuitable for repeated use. About 99 % of these compounds have been successfully separated from the acid solution by employing NaOH-treated magnetic particles developed during this investigation. It has been observed that by NaOH treatment, zeta potential of the magnetic particles could be increased from þ11 mV to þ37.5 mV; correspondingly, sugar removal efficiency was increased from 23.04 % to more than 99 %. Thus a direct correlation between the change in zeta potential of the particles and sugar separation efficiency has been observed.

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Effect of Process Factors on the Properties of Doxycycline Nanovesicles

Cited by 59 publications

References 16 publications

Docetaxel–Chitosan nanoparticles for breast cancer treatment: cell viability and gene expression study

Docetaxel–Chitosan nanoparticles for breast cancer treatment: cell viability and gene expression study

High‐Pressure Nebulization as Application Route for the Peritoneal Administration of siRNA Complexes

Magnetic particles for sugar separation from sulphuric acid solution generated during nano‐crystalline cellulose production

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