Effect of probiotics on intestinal regrowth and bacterial translocation after massive small bowel resection in a rat

Srugo, Isaac; Lurie, M.; Shaoul, Ron; Coran, Arnold G.; Shiloni, Eitan; Sukhotnik, Igor

doi:10.1016/j.jpedsurg.2007.03.035

Cited by 56 publications

(36 citation statements)

References 19 publications

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“…Mogilner et al (2007) have reported that Lactobacillus GG' did not affect proliferation of enterocytes in rats with short bowel syndrome. Similarly, it has been reported that Saccharomyces boulardii did not affect proliferation of enterocytes (Canonici et al, 2011).…”

Section: Cell Proliferation Migration and Turnovermentioning

confidence: 95%

“…In the same way, Di Giancamillo et al (2008) have reported that enterocyte proliferation increased in piglets supplemented with Pediococcus acidilactici. Mogilner et al (2007) have reported that there was a decrease in enterocyte death via apoptosis in rats with short bowel syndrome supplemented with Lactobacillus GG.…”

Section: Cell Proliferation Migration and Turnovermentioning

confidence: 99%

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The Impact of Probiotics on the Gastrointestinal Physiology

Matur¹,

Evren²

2012

New Advances in the Basic and Clinical Gastroenterology

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Section: Cell Proliferation Migration and Turnovermentioning

confidence: 95%

Section: Cell Proliferation Migration and Turnovermentioning

confidence: 99%

The Impact of Probiotics on the Gastrointestinal Physiology

Matur¹,

Evren²

2012

New Advances in the Basic and Clinical Gastroenterology

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“…Resected animals underwent 75 % small bowel resection from 5 cm distal to ligament of Treitz to 10 cm proximal to the ileo-cecal valve. We have shown in our previous studies that in 75 % bowel resection model almost all animals present with diarrhea demonstrate a 20 % decrease in body weight and lower plasma protein, cholesterol and TG levels, suggesting malabsorption and malnutrition [9,10]. All anastomoses were performed using interrupted sutures of 5-0 vicryl.…”

Section: Operative Proceduresmentioning

confidence: 99%

Effect of bowel resection on TLR signaling during intestinal adaptation in a rat model

et al. 2016

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“…Segments (B5 mm in length) of distal colon were placed into Universal fixative (4% paraformaldehyde, 1% glutaraldehyde in 0.1 M phosphate buffer) for further processing by electron microscopy. 28 Segments from the remainder of the distal colon were placed in 10% neutral-buffered formalin and processed for histological assessment. In some experimental groups, mice were injected intraperitoneally with a 10 mg/ml 5-bromo-2 0 -deoxyuridine (BrdU; Sigma) solution in PBS at a concentration of 5 ml/g 1 h before being killed.…”

Section: Infection Of Mice With C Rodentiummentioning

confidence: 99%

Detergent-resistant microdomains mediate activation of host cell signaling in response to attaching–effacing bacteria

Shen–Tu

Schauer

Jones

et al. 2010

Laboratory Investigation

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Enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes outbreaks of bloody diarrhea and the hemolytic-uremic syndrome. EHEC intimately adheres to epithelial cells, effaces microvilli and induces attaching-effacing (AE) lesions. Detergent-resistant microdomains (lipid rafts) serve as membrane platforms for the recruitment of signaling complexes to mediate host responses to infection. The aim of this study was to define the role of lipid rafts in activating signal transduction pathways in response to AE bacterial pathogens. Epithelial cell monolayers were infected with EHEC (MOI 100:1, 3 h, 371C) and lipid rafts isolated by buoyant density ultracentrifugation. Phosphoinositide 3-kinase (PI3K) localization to lipid rafts was confirmed using PI3K and anti-caveolin-1 antibodies. Mice with cholesterol storage disease Niemann-Pick, type C were used as in vivo models to confirm the role of lipid rafts in mediating signaling response to AE organisms. In contrast to uninfected cells, PI3K was recruited to lipid rafts in response to EHEC infection. Metabolically active bacteria and cells with intact cholesterol-rich microdomains were necessary for the recruitment of second messengers to lipid rafts. Recruitment of PI3K to lipid rafts was independent of the intimin (eaeA) gene, type III secretion system, and production of Shiga-like toxins. Colonization of NPC À/À colonic mucosa by Citrobacter rodentium and AE lesion formation were both delayed, compared with wild-type mice infected with the murine-specific AE bacterial pathogen. C. rodentium-infected NPC À/À mice had reduced colonic epithelial hyperplasia (64 ± 8.251 vs 112 ± 2.958 mm; Po0.05) and decreased secretion of IFN-g (17.6 ± 17.6 vs 71 ± 26.3 pg/ml, Po0.001). Lipid rafts mediate host cell signal transduction responses to AE bacterial infections both in vitro and in vivo. These findings advance the current understanding of microbial-eukaryotic cell interactions in response to enteric pathogens that hijack signaling responses mediated through lipid rafts.

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Effect of probiotics on intestinal regrowth and bacterial translocation after massive small bowel resection in a rat

Cited by 56 publications

References 19 publications

The Impact of Probiotics on the Gastrointestinal Physiology

The Impact of Probiotics on the Gastrointestinal Physiology

Effect of bowel resection on TLR signaling during intestinal adaptation in a rat model

Detergent-resistant microdomains mediate activation of host cell signaling in response to attaching–effacing bacteria

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