Effect of probiotics on diversity and function of gut microbiota in Moschus berezovskii

Yang, Chengcong; Huang, Weiqiang; Sun, Yaru; You, Lijun; Jin, Hao; Sun, Zhihong

doi:10.1007/s00203-021-02315-5

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…This indicates that the compound probiotics in the LS group had a better effect on the intestinal flora of calves than lactic acid in the L group or yeast in the S group. This is supported by the report that probiotic interventions promoted the gut microbiota's maturation through increasing microbial diversity and species richness, which could enhance the digestive and immune systems' development and build up disease resistance [21]. These results may be further associated with economic benefits for the cattle industry.…”

Section: Discussionmentioning

confidence: 69%

Compound Probiotics Improve the Diarrhea Rate and Intestinal Microbiota of Newborn Calves

Liu

Wang

Simujide

et al. 2022

Animals

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We evaluated the effects of probiotic compounds on the composition of the gut microbiota. Forty newborn calves were random allocated to the lactic acid bacteria + yeast group (LS group), lactic acid bacteria group (L group), yeast group (S group), and control group (D group). Probiotics containing Lactococcus lactis subsp. lactis, Pediococcus pentosaceus, Lactobacillus plantarum, Saccharomyces cerevisiae, and Kluyveromyces marxianus were fed to calves in the three treatment groups for 15 days. The feeding process lasted 15 days. Fecal samples were collected from all calves at the end of the trial and analyzed using high-throughput 16S rRNA sequencing. Totals of 1,029,260 high-quality reads and 420,010,128 bp of sequences were obtained. Among the four groups, the alpha diversity of gut microbes was significantly higher in newborn cattle in the LS group than in those in the L, S, and D groups. Overall, the dominant phyla were Firmicutes, Actinobacteria, and Bacteroidetes, whereas Bifidobacterium was the most abundant phylum in the gut of cattle in the LS group. Newborn calves from the compound probiotic groups had closely clustered gut bacterial communities and had lower rates of diarrhea. Overall, compound probiotics regulated the intestinal microbiota community structure of newborn calves and improved intestinal health. New information relevant to the prevention of diarrhea is provided by our research in newborn calves.

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Section: Discussionmentioning

confidence: 69%

Compound Probiotics Improve the Diarrhea Rate and Intestinal Microbiota of Newborn Calves

Liu

Wang

Simujide

et al. 2022

Animals

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“…Of interest was the identification of the rfb gene cluster ( rfbACBD ) within the second EPS operon of L. salivarius P1CEA3. These genes are responsible of the biosynthesis of dTDP-l-rhamnose, a crucial precursor in the synthesis of the cell wall of many bacteria [ 79 , 80 ] and the production of cell wall polysaccharides and rhamnose-containing EPS, in L. lactis [ 81 ]. This rhamnose-rich EPS is known to activate the human immune system by increasing the expression of many interleukins and cytokines [ 82 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Safety and Probiotic Traits from a Comprehensive Genome-Based In Silico Analysis of Ligilactobacillus salivarius P1CEA3, Isolated from Pigs and Producer of Nisin S

Sevillano,

Lafuente,

Peña

et al. 2023

Foods

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Ligilactobacillus salivarius is an important member of the porcine gastrointestinal tract (GIT). Some L. salivarius strains are considered to have a beneficial effect on the host by exerting different probiotic properties, including the production of antimicrobial peptides which help maintain a healthy gut microbiota. L. salivarius P1CEA3, a porcine isolated strain, was first selected and identified by its antimicrobial activity against a broad range of pathogenic bacteria due to the production of the novel bacteriocin nisin S. The assembled L. salivarius P1CEA3 genome includes a circular chromosome, a megaplasmid (pMP1CEA3) encoding the nisin S gene cluster, and two small plasmids. A comprehensive genome-based in silico analysis of the L. salivarius P1CEA3 genome reveals the presence of genes related to probiotic features such as bacteriocin synthesis, regulation and production, adhesion and aggregation, the production of lactic acid, amino acids metabolism, vitamin biosynthesis, and tolerance to temperature, acid, bile salts and osmotic and oxidative stress. Furthermore, the strain is absent of risk-related genes for acquired antibiotic resistance traits, virulence factors, toxic metabolites and detrimental metabolic or enzymatic activities. Resistance to common antibiotics and gelatinase and hemolytic activities have been discarded by in vitro experiments. This study identifies several probiotic and safety traits of L. salivarius P1CEA3 and suggests its potential as a promising probiotic in swine production.

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“…In local feeding farm, besides leaves, the feedstuffs for FMD are simple mixture of the chopped pumpkin, carrot and concentrate, and the concentrate mainly consisted of corn meal, soybean meal and bran, with different proportions in different farms. But the moldy leaves or rotten vegetables constantly lead to the intestinal diseases ( Wang et al, 2013 ), which further induced the defective of the FMD normal growth in breeding industry ( Zhu et al, 2012 ; Li et al, 2017 ; Yang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome

Gong

Song²,

Ai³

et al. 2023

Front. Microbiol.

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It is necessary to assess the appropriate dietary protein level of the forest musk deer (FMD), as nutritional needs are unclear. The microbiome in gastrointestinal tracts plays an important role in regulating nutrient utilization, absorption and host growth or development. Thus, we aimed to evaluate growth performance, nutrient digestibility and fecal microbiome of growing FMD supplied with different protein levels of diets. Eighteen 6-month-old male FMD with an initial weight 5.0 ± 0.2 kg were used in a 62-day trial. The animals were randomly distributed to three groups, the dietary crude protein (CP) level was 11.51% (L), 13.37% (M), and 15.48% (H). The results showed that the CP digestibility decreased as dietary CP level increased (p < 0.01). Compared with group L and H, FMD in M group has higher average daily gain, feed efficiency and neutral detergent fiber digestibility. For the fecal bacterial community, the percentage of Firmicutes was increased, Bacteroidetes was decreased and the diversity of microbiota significantly reduced (p < 0.05) with the increasing of dietary protein. The proportion of Ruminococcaceae_005, Ruminococcaceae_UCG-014 and uncultured_bacterium_f_Lachnospiraceae were significantly increased wtih rising CP, the proportions of Bacteroides and Rikenellaceae_RC9_gut_group were significantly decrease nevertheless at the genus level. The higher abundance of f_Prevotellaceae and g_Prevotellaceae_UCG_004 were found at M group by LEfSe analysis. The relative abundance of uncultured_bacterium_f_Ruminococcaceae was positively correlated with the average daily gain and feed conversion ratio (p < 0.05), whereas Family_XIII_AD3011_group was negatively correlated with feed conversion ratio (p < 0.05). The UPGMA tree showed L and M groups were closer in clustering relationship, while H group was clustered separately into a branch, which indicated that the bacterial structure had changed greatly with protein level increased from 13.37 to 15.48%. Overall, our results indicated that the optimum dietary CP for the growing FMD was 13.37%.

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Effect of probiotics on diversity and function of gut microbiota in Moschus berezovskii

Cited by 8 publications

References 41 publications

Compound Probiotics Improve the Diarrhea Rate and Intestinal Microbiota of Newborn Calves

Compound Probiotics Improve the Diarrhea Rate and Intestinal Microbiota of Newborn Calves

Evaluation of Safety and Probiotic Traits from a Comprehensive Genome-Based In Silico Analysis of Ligilactobacillus salivarius P1CEA3, Isolated from Pigs and Producer of Nisin S

Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome

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