Effect of probiotic lactobacilli on faecal enzyme and genotoxic activity in human faecal water in the presence of the carcinogen PhIP in vitro

Abstract

The average β‐glucuronidase activity for children was 0.48 ± 0.04 U/mg, for adults 0.75 ± 0.27 U/mg and for elderly 1.55 ± 0.06 U/mg. For β‐glucosidase, it was 0.19 ± 0.02 U/mg for children, 0.77 ± 0.26 U/mg for adults and 1.18 ± 0.27 U/mg for elderly. In the presence of probiotics, the highest decrease in genotoxicity was observed for Lactobacillus casei 0908 (to 7.99 ± 1.32) and Lactobacillus paracasei 0919 (to 6.19 ± 1.44) for all children. In adults, lower mean genotoxicity was regarded after incubation of PhIP with L. casei 0908 (it was 5.27 ± 1.13) and L. paracasei 0919 (it was o 6.01 ± 1.00). For elderly, statistically significant decrease was maintained after incubation of PhIP with L. casei 0900 (to 6.72 ± 2.67).

Growth and Survival of Probiotic Lactic Acid Bacteria in the Presence of Heterocyclic Aromatic Amines

“…A large number of reports exist to describe the adsorption or the binding by lactic acid bacteria (LAB) of a variety of food-borne HCAs in vitro; in some of those studies, a concomitant decrease in mutagenicity was reported [4,8,11,16,17,19,25]. Nevertheless, nothing is known about direct toxic effects of HCAs on certain species of probiotic lactic acid bacteria.…”

Potential Anticarcinogenic Effects of Lactic Acid Bacteria and Probiotics in Detoxification of Process-Induced Food Toxicants

“…As a final note, the decrease of NDMA was dependent on the medium, incubation time, phase of growth, strain type, pH, and NDMA concentration (63). Degrading of two nitroso compounds, including diphenylnitrosamine (DPN) and 1-nitrosopyrrolidine (NPR) by three strains of L.plantarum was determined.…”

“…L. casei 0908 and L. paracasei 0919 were the most effective in decreasing genotoxicity in children and adults. In the elderly group, L. casei 0900 was the most efficient strains (63). In another study by Klewicka et al the protective effect of feeding beetroot juice fermented with Lactobacillus brevis 0944 and L. paracasei 0920 against aberrant crypt foci (ACF) formation in rat colon in the presence of PhIP.…”

“…The most resistant strain was L. plantarum 0945, while L. casei 0908 and L. paracasei 0919 were the most sensitive to MeIQx and L. casei 0900 to IQ. Totally, it was implicated that probiotic bacteria are able to bind HCAs in human body and are removed in feces (63). In a study carried out by Stidl et The impact of pH on binding capacity has been illustrated in previous studies (38,45,65,66).…”

“…In the elderly group, the decrease range was from 83% (L. casei 0900) to 90% (L. paracasei 0919), and in the children group from 37% (L. casei 0900) to 55% (L. casei 0908) and in the adult group, there was no significant change in the activity of the enzymes. The greatest inhibition extent (64.5%) of fecal water genotoxicity by lactobacilli was achieved in the adult group (63). It is assumed that probiotics can adhere to colonocytes and restrict absorption of mutagens to the intestine (45) or decrease their bioavailability (68).…”