Effect of probiotic Bifidobacterium bifidum G9-1 on the relationship between gut microbiota profile and stress sensitivity in maternally separated rats

Fukui, Hirokazu; Oshima, Tadayuki; Tanaka, Yoshiki; Oikawa, Yosuke; Makizaki, Yutaka; Ohno, Hiroshi; Tomita, Toshihiko; Watari, Jiro; Miwa, Hiroto

doi:10.1038/s41598-018-30943-3

Cited by 69 publications

(69 citation statements)

References 41 publications

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“…Accumulating evidence has suggested that alteration in the gut microbiota is associated with the development of metabolic, psychological, and gastrointestinal functional disorders [ 11 , 12 , 13 , 14 ]. However, it has remained largely unknown how gut dysbiosis is involved in the pathophysiology in these disorders.…”

Section: Discussionmentioning

confidence: 99%

Alteration of Colonic Mucosal Permeability during Antibiotic-Induced Dysbiosis

Ran

Fukui

et al. 2020

IJMS

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Although dysbiosis is likely to disturb the mucosal barrier system, the mechanism involved has remained unclear. Here, we investigated alterations of colonic mucosal permeability and tight junction (TJ) molecules in mice with antibiotic-induced dysbiosis. Mice were orally administered vancomycin or polymyxin B for 7 days, and then fecal samples were subjected to microbial 16S rRNA analysis. The colonic mucosal permeability was evaluated by chamber assay. The colonic expression of TJ molecules and cytokines was examined by real-time RT-PCR, Western blotting, and immunohistochemistry. Caco2 cells were stimulated with cytokines and their transepithelial electric resistance (TEER) was measured. Vancomycin-treated mice showed significantly lower gut microbiota diversity than controls, and the same tendency was evident in polymyxin B-treated mice. The colonic mucosal permeability was significantly elevated in both vancomycin- and polymyxin B-treated mice. The expression of claudin 4 in the colonic mucosa was decreased in both vancomycin- and polymyxin B-treated mice. Colonic expression of TNF-α and/or IFN-γ was significantly increased in mice that had been administered antibiotics. TNF-α and IFN-γ stimulation dose-dependently decreased TEER in Caco2 cells. Antibiotic-induced dysbiosis is correlated with the enhancement in colonic tissue permeability, accompanied by a reduction in claudin 4 expression and enhancement in TNF-α and/or IFN-γ expression in mice.

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Section: Discussionmentioning

confidence: 99%

Alteration of Colonic Mucosal Permeability during Antibiotic-Induced Dysbiosis

Ran

Fukui

et al. 2020

IJMS

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“…In the current study, we note a greater impact of all dietary interventions on the gut microbiota than early‐life stress. Others have shown differences in the composition of the microbiota on postnatal day 20 that were amenable to microbial manipulation, but these were no longer evident in adulthood (Fukui et al., ). Similar to our study, differences in behaviour between MS and NS rats were noted in adulthood without linking to large differences in the microbiome.…”

Section: Discussionmentioning

confidence: 99%

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

O’Mahony

Neufeld

Waworuntu

et al. 2019

Eur J of Neuroscience

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Nutritional interventions targeting the microbiota–gut–brain axis are proposed to modulate stress‐induced dysfunction of physiological processes and brain development. Maternal separation (MS) in rats induces long‐term alterations to behaviour, pain responses, gut microbiome and brain neurochemistry. In this study, the effects of dietary interventions (milk fat globule membrane [MFGM] and a polydextrose/galacto‐oligosaccharide prebiotic blend) were evaluated. Diets were provided from postnatal day 21 to both non‐separated and MS offspring. Spatial memory, visceral sensitivity and stress reactivity were assessed in adulthood. Gene transcripts associated with cognition and stress and the caecal microbiota composition were analysed. MS‐induced visceral hypersensitivity was ameliorated by MFGM and to greater extent with the combination of MFGM and prebiotic blend. Furthermore, spatial learning and memory were improved by prebiotics and MFGM alone and with the combination. The prebiotic blend and the combination of the prebiotics and MFGM appeared to facilitate return to baseline with regard to HPA axis response to the restraint stress, which can be beneficial in times where coping mechanisms to stressful events are required. Interestingly, the combination of MFGM and prebiotic reduced the long‐term impact of MS on a marker of myelination in the prefrontal cortex. MS affected the microbiota at family level only, while MFGM, the prebiotic blend and the combination influenced abundance at family and genus level as well as influencing beta‐diversity levels. In conclusion, intervention with MFGM and prebiotic blend significantly impacted the composition of the microbiota as well as ameliorating some of the long‐term effects of early‐life stress.

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“…Approximately 500 μL of whole blood was collected in 1.5-mL microcentrifuge tubes containing 10 μL of EDTA (ethylenediaminetetraacetic acid). The serum was obtained by centrifugation for 10 min in a refrigerated centrifuge at 4 °C and stored at −80 °C for further analysis [ 32 , 33 ]. The serum corticosterone level was quantified using a commercially available corticosterone ELISA kit (Arigo, ARG80652, Hsinchu, Taiwan, China), following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Effect of a Synbiotic Containing Lactobacillus paracasei and Opuntia humifusa on a Murine Model of Irritable Bowel Syndrome

Seong

Lee

et al. 2020

Nutrients

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The administration of a combination of probiotics and prebiotics is expected to be a promising strategy for improving irritable bowel syndrome (IBS) symptoms. This study aimed to investigate the efficacy of a synbiotic containing Lactobacillus paracasei and Opuntia humifusa extract for symptomatic improvement of IBS in a murine model and to evaluate the mechanism underlying the beneficial effects of this synbiotic. A total of 20 male Wistar rats aged 8 weeks with IBS induced by restraint stress were assigned into four groups and administered L. paracasei as a probiotic and O. humifusa extract as a prebiotic for 4 weeks. The primary outcome was stool consistency at week 4. To evaluate the mechanism underlying the beneficial effects of the synbiotic, fecal microbial analysis was conducted, and the serum corticosterone levels, tumor necrosis factor-α (TNF-α) levels in the colon tissue, and expression of tight junction proteins were investigated. All three treatment groups showed significantly lower scores for stool consistency than the control group at week 4 (all p < 0.001). When compared with the control group, the synbiotic groups showed a significantly greater abundance of L. paracasei in fecal microbial analysis, lower serum corticosterone levels, lower TNF-α levels in the colon tissue, and higher expression of tight junction proteins. This novel synbiotic containing L. paracasei and O. humifusa extract can improve the stool consistency in a murine model of IBS. It may be a promising treatment option for IBS, and human studies are warranted.

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Effect of probiotic Bifidobacterium bifidum G9-1 on the relationship between gut microbiota profile and stress sensitivity in maternally separated rats

Cited by 69 publications

References 41 publications

Alteration of Colonic Mucosal Permeability during Antibiotic-Induced Dysbiosis

Alteration of Colonic Mucosal Permeability during Antibiotic-Induced Dysbiosis

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

Effect of a Synbiotic Containing Lactobacillus paracasei and Opuntia humifusa on a Murine Model of Irritable Bowel Syndrome

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