Effect of Printing Process Parameters on the Shape Transformation Capability of 3D Printed Structures

Pivar, Matej; Gregor-Svetec, Diana; Muck, Deja

doi:10.3390/polym14010117

Cited by 23 publications

(11 citation statements)

References 42 publications

(54 reference statements)

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“…The quantification of the filament dimension (Figure 6C) revealed a mean dimension of 281μm ± 41 μm higher than the noozle diamenter (200 μm). This could be expected as typical results of the technique where after printing the filament tends to increase its dimension due to stress relaxation [42,43]. The printed structures were proven to be injectable and to retain the shape once ejected.…”

Section: Pneumatic Extrusion Printingsupporting

confidence: 60%

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli¹

2022

Preprint

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Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light. This allows the structuring of this material throught additive manufacturing techniques and then to easily prototype patient specific construct. In this study we used Sil-MA to produce single layer crosslinked structures that can be withdrawal and ejected recovering their shape after rehydration. A complete chemical and physical characterization of the material has been conducted. Additionally, we tested the material biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility to use it in future trials. The material was also tested to verify its ability to support the osteogenesis. Two different additive manufacturing techniques have been tested (a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique) to develop Sil-MA grid. Finally, we provide a proof-of-concept that the printed Sil-MA structures are injectable.

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Section: Pneumatic Extrusion Printingsupporting

confidence: 60%

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli¹

2022

Preprint

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“…The quantification of the filament dimension ( Figure 6 C) revealed a mean dimension of 281 ± 41 μm, approximately 40% higher than the nozzle diameter (200 μm). This is an expected phenomenon since, after extrusion, the filament increases its dimension due to stress relaxation [ 40 , 41 ]. The printed structures were proven to retain their shape once ejected from a nozzle.…”

Section: Resultsmentioning

confidence: 99%

“…In fact, GelMA has been recently printed with a resolution of 200 μm by a DLP printer with a maximum resolution of 50 μm [ 46 ]. When pneumatic extrusion was used, an increase in the dimension occurred in the Sil-MA structures and should be attributed to the flow stresses relaxation, a phenomenon widely discussed in the literature [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli

Petretta

Grigolo

et al. 2022

Gels

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Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light, which makes this material applicable in additive manufacturing techniques and allows the prototyping and development of patient-specific 2D or 3D constructs. In this study, we produced a thin grid structure based on crosslinked Sil-MA that can be withdrawn and ejected and that can recover its shape after rehydration. A complete chemical and physical characterization of Sil-MA was first conducted. Additionally, we tested Sil-MA biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility of using it in future trials. Sil-MA was also tested to verify its ability to support osteogenesis. Overall, Sil-MA was shown to be biocompatible and osteoconductive. Finally, two different additive manufacturing technologies, a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique, were used to develop a Sil-MA grid construct. A proof-of-concept of its shape-memory property was provided. Together, our data support the hypothesis that Sil-MA grid constructs can be injectable and applicable in bone regeneration applications.

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“…It is well documented that TPU is harder to print due to its exibility causing poor bonding [22,23].…”

Section: Characterisation Of 3d Scaffoldmentioning

confidence: 99%

Osteogenic differentiation capacity of dental pulp stem cells on 3D printed polyurethane/boric acid scaffold

Çelebi‐Saltik

Babadag

Ballıkaya

et al. 2023

Preprint

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Additive manufacturing is growing in the area of dentistry and orthopedics due to the potential for the fabrication of individual implants. In this study, fused deposition modeling which is the most popular method was used to produce 3D scaffolds having a grid pattern from the polyurethane (PU) filament. Then, this scaffold was coated with boric acid (BA) with the thermionic vacuum arc technique. The microstructure analysis showed the macro-pores having a dimension of ~ 0.16 mm2. The BA coating increased the roughness in adverse decreased the wettability. The presence of BA on the scaffold before and after cell culture was confirmed by FESEM-EDS and ATR-FTIR. The Cell proliferation and osteogenic differentiation capacity of dental pulp stem cells (DPSCs) on uncoated and coated printed 3D PU scaffolds were also investigated. On the third day, cell viability was found to be higher (1.3-fold) in the groups containing BA. However, on the seventh day, the increase in cell proliferation in the PU+BA group was found to be less than in the other groups. According to Ca deposition analysis and Alizarin Red staining, PU+BA increased the calcium accumulation in the cells in both osteogenic induced and non-induced conditions at day-14. According to gene expression analysis, the Runx2 expression was not detected in PU+BA groups with and without differentiation medium (p ≤0.05). The expression of OCN was persistently increased up to 21-fold and 48-fold in PU and PU+BA in osteogenic differentiation medium group after 14 days compared to control group (p ≤0.05). DSPP expression was observed only in PU+BA in osteogenic differentiation medium group. In line with the results that we have obtained, our 3D printed synthesized scaffolds have properties to trigger the differentiation of DPSCs cells in terms of osteogenicity.

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Effect of Printing Process Parameters on the Shape Transformation Capability of 3D Printed Structures

Cited by 23 publications

References 42 publications

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Osteogenic differentiation capacity of dental pulp stem cells on 3D printed polyurethane/boric acid scaffold

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