The current research investigated the dyeing properties on cotton and PLA fabrics of the sulphatoethylsulphone reactive-disperse dye synthesized from coupling reaction between aminophenyl-4-(β-sulphatoethylsulphone) diazonium salt and N,N-diethyl-m-toluidine, a preliminary study aiming to develop the dye for one-bath dyeing for PLA/cotton blended fabric. From the study, the optimum dyeing condition of the synthesized dye on PLA fabric was at pH 6 and 110°C. The synthesized dye performed very well on PLA rather than cotton. The dyed PLA fabric also displayed excellent color fastnesses to washing and crocking. A study on cotton dyeing gave the information that the dyeing ability of the dye was enhanced by increasing dyeing temperature and the amounts of added salt and alkali. The dyed cotton fabric exhibited a moderate to good color fastnesses to washing and crocking. A nearly similar shade obtained on PLA and cotton encourages a dyeing process development for PLA/cotton blended fabric.