Effect of pressure on the natural radiative lifetimes of anthracene derivatives in solution

Hirayama, Satoshi; Yasuda, Hiroya; Okamoto, Masami; Tanaka, Futoshi

doi:10.1021/j100161a008

Cited by 28 publications

(30 citation statements)

References 1 publication

(1 reference statement)

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“…We have chosen a well known and widely studied hydrophobic dye with a high quantum yield: 9,10-diphenylanthracene (DPA). [64][65][66][67][68] Its operating distance for energy transfer to cyclam-copper complex is in the 2 nm range. Since the energy transfer between the entrapped dye and the metal complexes that form at the NP surface will occur over medium distances, the quenching efficiency will be a powerful indicator of the dye accessibility inherent in nanosized-templates.…”

Section: Full Papermentioning

confidence: 99%

Fluorescence Quenching upon Binding of Copper Ions in Dye‐Doped and Ligand‐Capped Polymer Nanoparticles: A Simple Way to Probe the Dye Accessibility in Nano‐Sized Templates

Gouanvé

Schuster

Allard

et al. 2007

Adv Funct Materials

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The synthesis and properties of well‐defined core–shell type fluorescent metal‐chelating polymer nanoparticles NP, in the 15 nm diameter range, with a fluorophore (9,10‐diphenylanthracene: DPA) entrapped in the particle core and a selective ligand (1,4,8,11‐tetraazacyclotetradecane: Cyclam), grafted onto the surface are presented. NPs with different number of dye‐per‐particle are readily obtained by entrapment of the fluorophore within the polymer core. The ligand‐coated NPs exhibit a high affinity for Cu2+ ions in aqueous solution and quenching of the DPA fluorescence is observed upon binding of copper. The quenching of fluorescence arises through energy transfer (FRET) from the dye to the copper‐cyclam complexes that form at the NP surface with an operating distance (d) in the 2 nm range. A simple core–shell model accounts for the steady‐state and time‐resolved fluorescence titration experiments: dye molecules located in the outer sphere (thickness d) of the NPs are quenched while the fluorescence of dyes embedded more deeply is not affected by the binding of copper ions. The observed high quenching efficiency (60–65 %), which is tightly correlated to the volumic and microstructural features of the NPs, shed light on the enhanced accessibility inherent in nano‐sized templates. The response towards different metal ions was investigated and this confirmed the selectivity of the nanoparticle template‐assembled sensor for cupric ions.

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Section: Full Papermentioning

confidence: 99%

Fluorescence Quenching upon Binding of Copper Ions in Dye‐Doped and Ligand‐Capped Polymer Nanoparticles: A Simple Way to Probe the Dye Accessibility in Nano‐Sized Templates

Gouanvé

Schuster

Allard

et al. 2007

Adv Funct Materials

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“…The natural radiative lifetime explicitly depends on the refractive index due to the polarizability of the medium surrounding the fluorophore. Indeed, a number of experimental studies verify the dependence of the natural radiative lifetime 0 as well as the fluorescence lifetime of dyes on the refractive index (Olmsted III, 1976;Hirayama and Phillips, 1980;Lampert et al, 1983;Hirayama et al, 1990Hirayama et al, , 1991Magde et al, 1999), and recently Toptygin et al (2002) studied the effect of the refractive index on the radiative rate constant of tryptophan residues. By varying the refractive index with pressure (Hirayama et al, 1991), temperature (Lampert et al, 1983), or by using different solvents (Lampert et al, 1983), the radiative rate constant k r of anthracene derivatives scales approximately with the square of the refractive index.…”

Section: Introductionmentioning

confidence: 97%

Imaging the Environment of Green Fluorescent Protein

Suhling

Siegel

Phillips

et al. 2002

Biophysical Journal

245

210

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An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also probe their environment. Such a technique is demonstrated here using fluorescence lifetime imaging of green fluorescent protein (GFP). We first show, by time-correlated single-photon counting, that the fluorescence decay of GFP depends on the local refractive index. This is in agreement with the Strickler Berg formula, relating the Einstein A and B coefficients for absorption and spontaneous emission in molecules. We then quantitatively image, by wide-field time-gated fluorescence lifetime imaging, the refractive index of the environment of GFP. This novel approach paves the way for imaging the biophysical environment of specific GFP-tagged proteins in live cells.

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“…As previously noted (Jalviste et al 2020), an implicit assumption behind model ( 1) is a weak dependence on pressure of k0 relative to other rate constants. In general, all the processes that contribute into the non-photochemical decay rate constant are prone to change with pressure, while only the radiative lifetime dependence has a well-justified predictable form (Olmsted 1976;Hirayama and Phillips 1980;Hirayama et al 1991).…”

Section: Discussionmentioning

confidence: 99%

High-pressure tuning of primary photochemistry in bacterial photosynthesis: membrane-bound versus detergent-isolated reaction centers

et al. 2020

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While photosynthesis thrives at close to normal pressures and temperatures, it is presently well known that life is similarly commonplace in the hostile environments of the deep seas as well as around hydrothermal vents. It is thus imperative to understand how key biological processes perform under extreme conditions of high pressures and temperatures. Herein, comparative steady-state and picosecond time-resolved spectroscopic studies were performed on membrane-bound and detergent-purified forms of a YM210W mutant reaction center (RC) from Rhodobacter sphaeroides under modulating conditions of high hydrostatic pressure applied at ambient temperature. A previously established breakage of the lone hydrogen bond formed between the RC primary donor and the protein scaffold was shown to take place in the membrane-bound RC at an almost 3 kbar higher pressure than in the purified RC, confirming the stabilizing role of the lipid environment for membrane proteins. The main change in the multiexponential decay of excited primary donor emission across the experimental 10 kbar pressure range involved an over two-fold continuous acceleration, the kinetics becoming increasingly mono-exponential. The fastest component of the emission decay, thought to be largely governed by the rate of primary charge separation, was distinctly slower in the membrane-bound RC than in the purified RC. The change in character of the emission decay with pressure was explained by the contribution of charge recombination to emission decreasing with pressure as a result of an increasing free energy gap between the charge-separated and excited primary donor states.Finally, it was demonstrated that, in contrast to a long-term experimental paradigm, adding a combination of sodium ascorbate and phenazine methosulfate to the protein solution potentially distorts natural photochemistry in bacterial RCs.

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Effect of pressure on the natural radiative lifetimes of anthracene derivatives in solution

Cited by 28 publications

References 1 publication

Fluorescence Quenching upon Binding of Copper Ions in Dye‐Doped and Ligand‐Capped Polymer Nanoparticles: A Simple Way to Probe the Dye Accessibility in Nano‐Sized Templates

Fluorescence Quenching upon Binding of Copper Ions in Dye‐Doped and Ligand‐Capped Polymer Nanoparticles: A Simple Way to Probe the Dye Accessibility in Nano‐Sized Templates

Imaging the Environment of Green Fluorescent Protein

High-pressure tuning of primary photochemistry in bacterial photosynthesis: membrane-bound versus detergent-isolated reaction centers

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