Effect of preservation procedures on the body shape of the golden mojarra, Diapterus aureolus (Actinopterygii: Perciformes: Gerreidae), and its repercussions in a taxonomic study

Vergara‐Solana, Francisco Javier; Rodríguez, F. Millán; Cruz‐Agüero, José De La

doi:10.3750/aip2014.44.1.08

Cited by 10 publications

(12 citation statements)

References 19 publications

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“…By presenting multiple staging methods and descriptions of development, additional tools are provided to researchers that complement morphological stages. A common practice in embryo studies involves preserving samples in fixatives such as ethanol or formaldehyde; which have been reported to cause embryo shrinkage (Melo et al ., ; Vergara‐Solana et al ., ). This study presents the effects of two common fixatives, ethanol and formalin, on embryo morphometrics to assist researchers in both the staging and use of embryos for morphological measurements following fixation.…”

Section: Discussionmentioning

confidence: 97%

“…Several studies have investigated the combined effects of formalin and ethanol on species of fishes post hatch. Other studies have reported similar embryo shrinkage trends (Melo et al ., ; Vergara‐Solana et al ., ). The change in L T from fixed (10% formalin followed by 70% ethanol) to unfixed individuals has been found to range from 5·7% in golden mojarra Deckertichthys aureolus (Jordan & Gilbert 1882) (Vergara‐Solana et al ., ) to 11·77% in juvenile Brazilian silverside Atherinella brasiliensis (Quoy & Gaimard 1825) (Melo et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…In addition, research often necessitates the use of preserved samples for analysis. Embryo fixation in preservatives such as formalin and ethanol has been reported to affect embryo size and can therefore reduce the reliability of a staging system based solely on fixed measurements (Parker, 1963;Mabee et al, 1998;Smith & Walker, 2003;Melo et al, 2010;Vergara-Solana et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Embryonic development of lake whitefish Coregonus clupeaformis: a staging series, analysis of growth and effects of fixation

Sreetharan

Thome

Mitz

et al. 2015

Journal of Fish Biology

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A reference staging series of 18 morphological stages of laboratory reared lake whitefish Coregonus clupeaformis is provided. The developmental processes of blastulation, gastrulation, neurulation as well as development of the eye, circulatory system, chromatophores and mouth are included and accompanied by detailed descriptions and live imaging. Quantitative measurements of embryo size and mass were taken at each developmental stage. Eggs were 3·19 ± 0·16 mm (mean ± s.d.) in diameter at fertilization and embryos reached a total length (LT ) of 14·25 ± 0·41 mm at hatch. Separated yolk and embryo dry mass were 0·25 ± 0·08 mg and 1·39 ± 0·17 mg, respectively, at hatch. The effects of two common preservatives (formalin and ethanol) were examined throughout development and post hatch. Embryo LT significantly decreased following fixation at all points in development. A correction factor to estimate live LT from corresponding fixed LT was determined as live LT = (fixed LT )(1·025) . Eye diameter and yolk area measurements significantly increased in fixed compared with live embryos up to 85-90% development for both measurements. The described developmental stages can be generalized to teleost species, and is particularly relevant for the study of coregonid development due to additionally shared developmental characteristics. The results of this study and staging series are therefore applicable across various research streams encompassing numerous species that require accurate staging of embryos and descriptions of morphological development.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Embryonic development of lake whitefish Coregonus clupeaformis: a staging series, analysis of growth and effects of fixation

Sreetharan

Thome

Mitz

et al. 2015

Journal of Fish Biology

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“…Specimens were identified using morphological characteristics described by Bussing () and Castro‐Aguirre, Espinosa‐Perez, and Schmitter‐Soto (). After identification, selected fresh specimens were photographed to avoid deformation in size and shape caused by fixation and preservation procedures (Berbel‐Filho, Jacobina, & Martinez, ; Martinez, Berbel‐Filho, & Jacobina, ; Vergara‐Solana, García‐Rodríguez, & De La Cruz‐Agüero, ). Specimens, tissue samples, otoliths and urohyal bones used for molecular analysis and geometric morphometrics were catalogued and deposited in the Ichthyologic Collection of IPN–CICIMAR (http://coleccion.cicimar.ipn.mx), where they are available upon request.…”

Section: Methodsmentioning

confidence: 99%

Supporting the existence of two isolated evolutionary lineages ofGerres(Perciformes: Gerreidae) in America

et al. 2019

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For many years in America, many authors have considered that the Gerres genus contained a single species, the yellowfin mojarra Gerres cinereus (Walbaum, 1792); however, others have recognized two species in this genus in the Neotropics. In this study, specimens of G. cinereus (sensu lato) caught throughout its geographic range on both coasts of America were compared for the first time to analyse divergence among the sampled populations. A genetic analysis based on DNA sequences from three mitochondrial genes and one nuclear gene revealed a clear separation of two clades, one in the Pacific and one in the Atlantic. Geometric morphometric differences, based on body shape, sagittae otoliths and urohyal bone features, were significant between the two groups, including separation by an accurate classification a posteriori by a canonical variate analysis (CVA) > 82% in all data sets (overall mean >91%). Integration of the results of these approaches indicates that there is sufficient evidence to support species status for Gerres simillimus Regan, 1907, and it should be considered a valid species in the neotropical Pacific.

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“…This was to reduce the effect of potential weight reduction from dehydration and possible distortion of samples due to long-term storage in ethyl alcohol (Andriguetto & Haimovici 1988;Black & Dodson 2003;Bucklin 2009;Díaz-Viloria et al 2005;DiStefano et al 1994;Vergara-Solana et al 2014).TL was measured along the dorsal surface from the anterior tip of the rostrum to the posterior end of the telson and measured to the nearest FIGURE 1. (Pauly 1984), where W is the wet weight (WW) and L is the total length (TL).…”

Section: Length-weight Measurements and Analysesmentioning

confidence: 99%

Length-Weight Relationships of Acetes spp. Sampled Along the West Coast of Peninsular Malaysia

Boon¹,

Han²,

Khoo³

2015

JSM

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Effect of preservation procedures on the body shape of the golden mojarra, Diapterus aureolus (Actinopterygii: Perciformes: Gerreidae), and its repercussions in a taxonomic study

Cited by 10 publications

References 19 publications

Embryonic development of lake whitefish Coregonus clupeaformis: a staging series, analysis of growth and effects of fixation

Embryonic development of lake whitefish Coregonus clupeaformis: a staging series, analysis of growth and effects of fixation

Supporting the existence of two isolated evolutionary lineages ofGerres(Perciformes: Gerreidae) in America

Length-Weight Relationships of Acetes spp. Sampled Along the West Coast of Peninsular Malaysia

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