Effect of Prenatal Exposure to Ethanol on Intestinal Development of Rat Fetuses

Abstract

Background:Chronic alcoholism in pregnant animals and humans lead to general growth impairment in their offspring, which show multiple birth defects and delayed grown (fetal alcohol syndrome). Here we study the maturation of the intestine under the effect of chronic exposure to ethanol in utero together with associated malnutrition.Methods:Lactase, acid β‐galactosidase, maltase, and alkaline phosphatase activity profiles were monitored in 18‐, 19‐, 20‐, and 21‐day‐old fetuses from rats kept under three nutritional treatments before and during gestation: alcohol‐treated (25% ethanol in drinking water), fiber‐treated (50% cellulose‐diluted diet) as a control of the malnutrition associated with chronic alcoholism, and control or normal diet. Serum corticosterone determination and lactase immunolocalization were carried out. To detect possible direct effects of ethanol during the period of mucosa development, intestinal explants from 18‐, 19‐, and 20‐day‐old control fetuses were cultured either in the basal medium alone or in a medium containing 25 mM ethanol for 72, 48, and 24 h of incubation, respectively.Results:Following chronic ethanol exposure in utero, intestinal weight and brush‐border protein content and the specific activities of lactase, acid β‐galactosidase, maltase, and alkaline phosphatase were significantly lower than those of nutritional controls. Organ culture results, under the assay conditions stated, did not show a direct effect of ethanol 25 mM on prenatal mucosal functionality.Conclusions:All these results suggest that maternal malnutrition is not primarily responsible for the impaired intestinal maturation in rat fetuses from alcohol‐treated mothers; indirect effects of ethanol and/or its derivatives throughout embryo‐fetal development could be necessary to promote this intestinal delay.