Effect of prematuration and maturation with fibroblast growth factor 10 (FGF10) on in vitro development of bovine oocytes

Diógenes, M. N.; Guimarães, A. L. S.; Leme, L. O.; Maurício, Machaim Franco; Dode, M. A. N.

doi:10.1016/j.theriogenology.2017.06.004

Cited by 15 publications

(16 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also found that FGF10 affected these biological processes in a dose-dependent manner. However, the opposite results were reported in bovines Diógenes, Guimarães, Leme, Maurício & Dode, 2017;K. Zhang et al 2010), showing that the presence of FGF10 during IVM had no effect on the percentage of oocytes reaching metaphase II at 0.5 ng/ml.…”

Section: Discussionmentioning

confidence: 65%

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

Wang

Baloch

Zhang

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

The fusion of sperm and oocytes determines the fertilization competence and subsequent development of embryos, which, in turn, can be affected by various proteins and DNA methylation. However, several factors in this whole regulation process remain unknown, especially in yaks. Here, we report that fibroblast growth factor 10 (FGF10) is an important growth factor that can enhance the maturation rate of yak oocytes and the motility of frozen spermatozoa. Subsequent blastocyst quality was also improved by increasing the total cell number and level of pregnancy‐associated protein in blastocysts. These effects were significantly high in the group that received the 5 ng/ml FGF10 treatment, during both in vitro maturation (IVM) and capacitation. Our data show that the effects of FGF10 were dose‐dependent at vital steps of embryogenesis in vitro. Furthermore, quantitative polymerase chain reaction, western blot analysis, and immunofluorescence demonstrated that the levels of CD9, CD81, DNMT1, and DNMT3B in both mature cumulus‐oocyte complexes and capacitated sperms were regulated by FGF10, which was also highly expressed in the group treated with 5 ng/ml FGF10 during both IVM and capacitation. From our present study, we concluded that FGF10 promotes yak oocyte fertilization competence and subsequent blastocyst quality, and could also regulate CD9, CD81, DNMT1, and DNMT3B to optimize sperm–oocyte interactions and DNA methylation during fertilization.

show abstract

Section: Discussionmentioning

confidence: 65%

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

Wang

Baloch

Zhang

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…Additionally, to optimize assisted reproductive technologies (ARTs) in mammals, the morphology of cumulus expansion can be potentially helpful as a non-invasive oocyte competence marker [ 4 ]. Recent studies support the evidence of the significant correlation between fibroblast growth factor 10 (FGF10) and the extent of cumulus expansion in bovines [ 5 , 6 ]. In addition to this growth factor, reproductive hormones such as FSH, estrogen and prostaglandin E2 (PGE2), can also induce cumulus expansion and meiosis resumption in mammals [ 7 – 10 ], however, not much is known about their roles in large livestock, especially in the yaks ( Bos grunniens ).…”

Section: Introductionmentioning

confidence: 83%

“…Cumulus expansion was measured following the methods described by Diógenes et al [ 5 , 6 ]. Briefly, measurement was done for each COC after selection and then maturation by using ImageJ software ( http://imagej.nih.gov/ij ).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation

Wang

Zhang

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

The estrogen-signalling pathway is critical for normal follicular development; however, little is known about its importance during in vitro maturation (IVM) in large animals, particularly yaks ( Bos grunniens ). Through the present study, we aimed to determine the mechanisms underlying estrogen involvement in cumulus expansion and the subsequent development of cumulus-oocyte complexes (COCs). COCs were cultured in the maturation medium supplemented with different concentrations (10 −6 –10 −3 mM) of 17β-estradiol (E2) or its receptor antagonist, fulvestrant, and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and western blot were performed to determine the expression of cumulus-expansion related factors and oocyte-secreted factors (OSFs). The cumulus expansion of COCs was observed using an inverted microscope, and COCs developmental ability were judged by the evaluation of cleavage and blastulation rates per inseminated oocytes by IVF, and the number of cells in the blastocyst. Cumulus expansion increased with 10 −6 –10 −3 mM E2, but decreased with fulvestrant. HAS2 , PTGS2 , PTX3 and OSFs expression increased in the 10 −6 –10 −3 mM E2 groups. Significantly higher cleavage and blastocyst rates were observed in the 10 −4 mM E2 group than in the fulvestrant and 0 mM E2 groups. Moreover, in the 10 −4 mM group, blastocysts at 7 days had higher cell counts than the other groups. In conclusion, the increase in cumulus expansion and subsequent oocyte development after the addition of E2 to IVM medium may have resulted from increased cumulus-expansion-related factor expression and OSF levels.

show abstract

“…For instance, blastocysts with higher number of cells or embryo with better developmental kinetics. In other cases, results showed no significant improvement over the current standard in vitro embryo production system (Jee et al, 2009;Gharibi et al, 2013;Guimaraes et al, 2015;Diogenes et al, 2017).…”

Section: Controlling Campmentioning

confidence: 94%

The variable success of in vitro maturation: can we do better?

Luciano¹,

Franciosi²,

Barros³

et al. 2018

Anim Reprod

View full text Add to dashboard Cite

The efficiency of in vitro assisted reproductive technologies, consisting of the transfer of embryos obtained in vitro through in vitro maturation, in vitro fertilization and early embryo culture is still limited. The quality of the oocytes is pivotal for assisted reproductive efficiency and the maturation of the oocyte represents the first key limiting step of the in vitro embryo production system. At the time of removal from the antral follicles, the oocyte is still completing the final growth and differentiation steps, needed to provide the so-called developmental competence, i.e. the machinery required to sustain fertilization and embryo development. In mono-ovular species only one oocyte per cycle is available for procreation, therefore the current assisted reproduction techniques strive to overcome this natural boundary. However, the success is still limited and overall the effectiveness does not exceed the efficiency achieved in millions of years of mammalian evolution. One of the problems lies in the intrinsic heterogeneity of the oocytes that are subjected to in vitro maturation and in the lack of dedicated in vitro approaches to finalize the differentiation process. In this review we will try to overview some of the salient aspects of current practices by emphasizing the most critical and fundamental features in oocyte differentiation that should be carefully considered for improving current techniques.

show abstract

Effect of prematuration and maturation with fibroblast growth factor 10 (FGF10) on in vitro development of bovine oocytes

Cited by 15 publications

References 53 publications

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation

The variable success of in vitro maturation: can we do better?

Contact Info

Product

Resources

About