Effect of Preliminary Load of Macrophages with Silicium Dioxide on Phagocytosis of BCG Strain Micobacteria by Macrophages and Antimicrobial Activity

Архипов, С. А.; Shkurupy, V. A.; Bugrimova, Yu. S.

doi:10.1007/s10517-010-0986-0

Cited by 8 publications

(9 citation statements)

References 7 publications

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“…Because PRRs, such as C-type lectin receptors, SRs, TLRs, NOD-like receptors, and RIG-I like receptors, play a prominent role in the activation of AMs and subsequent cross-talk with innate and adaptive immune cells, how a macrophage reacts to a given stimulus depends greatly on the diverse range of PRRs expressed on the cell’s surface ( 43 , 44 ). In the case of concomitant or sequential exposure to two distinct pathogens, the capacity of macrophages to recognize, phagocytose, and appropriately respond to a second stimuli may be compromised by stimulant-induced changes in the profile of PRRs ( 45 ) – thus altering susceptibility to disease. TLRs, which recognize microbial molecules, are major triggers of innate responses (e.g., enhanced costimulatory molecule expression, cytokine secretion, production of reactive oxygen species, and antimicrobial mediators) and thus modulate adaptive immunity by influencing macrophage functions.…”

Section: Discussionmentioning

confidence: 99%

Acute Exposure to Crystalline Silica Reduces Macrophage Activation in Response to Bacterial Lipoproteins

et al. 2016

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Numerous studies have examined the relationship between alveolar macrophages (AMs) and crystalline silica (SiO2) using in vitro and in vivo immunotoxicity models; however, exactly how exposure to SiO2 alters the functionality of AM and the potential consequences for immunity to respiratory pathogens remains largely unknown. Because recognition and clearance of inhaled particulates and microbes are largely mediated by pattern recognition receptors (PRRs) on the surface of AM, we hypothesized that exposure to SiO2 limits the ability of AM to respond to bacterial challenge by altering PRR expression. Alveolar and bone marrow-derived macrophages downregulate TLR2 expression following acute SiO2 exposure (e.g., 4 h). Interestingly, these responses were dependent on interactions between SiO2 and the class A scavenger receptor CD204, but not MARCO. Furthermore, SiO2 exposure decreased uptake of fluorescently labeled Pam2CSK4 and Pam3CSK4, resulting in reduced secretion of IL-1β, but not IL-6. Collectively, our data suggest that SiO2 exposure alters AM phenotype, which in turn affects their ability to uptake and respond to bacterial lipoproteins.

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Section: Discussionmentioning

confidence: 99%

Acute Exposure to Crystalline Silica Reduces Macrophage Activation in Response to Bacterial Lipoproteins

et al. 2016

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“…The tuberculosis loading in rats was consistent with the progress of silicosis. Interestingly, an in vitro study by Shkurupy et al 13,14 demonstrated that preliminary loading of macrophages with silicium dioxide reduced their antibacterial activity, implying that the cytotoxicity of silica dust to macrophage was the main reason for the damage to the anti-tuberculosis function. However, little was known about the detail injury mechanism and immune regulation.…”

Section: Discussionmentioning

confidence: 99%

Establishment of rat model of silicotuberculosis and its pathological characteristic

Dong

Yabo

et al. 2014

Pathogens and Global Health

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Objective: To establish different stages of silicosis rat model complicated with tuberculosis infection, and compare the pathological characteristics and analyze the impact of silicosis on tuberculosis infection. Methods: SD rats were subjected to intratracheal administration of silica with non-exposure method at the 1st, 30th, or 60th day. At the 50th day, the rats were injected with the suspension of H37Rv (a virulent standard strain of Mycobacterium tuberculosis) via tail-vein. After 40 days post-infection, rats were sacrificed, the lung tissues were isolated, and paraffin was embedded and sectioned. The sections were treated using HE staining for structure observation, acid fast stain of Ziehl-Neelsen for bacterial detection, and Warthin-Starry silver staining for displaying the distribution of dust particles. The bacterial load was quantified by colony counting. Results: Primary to tertiary silicosis could be discovered at the 30th, 60th, and 90th day of post-infection. The rats could be infected by injection of M. tuberculosis via tail vein, with tuberculosis load and the degree of lung tissue lesions positively correlated with silicosis. Conclusion: The rat model of silicotuberculosis was established successfully, which facilitated understanding the 'cross-talk' of silicosis and tuberculosis during the process they drive each other.

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“…Immunocytochemical studies of MP were carried out by the indirect immunocytochemical method [8]. Macrophages in cultures were fi xed with neutral formalin (4% water solution), the enzymes were retrieved with Triton X-100 (0.3% solution in phosphate buffer, pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

“…Studies of MP secretory activity were carried out by the same protocol as studies of MP expression of enzymes [8]. Sterile millipore membranes of standard size (10×10 mm) were put into Petri dishes (40 mm) instead of slides.…”

Section: Methodsmentioning

confidence: 99%

Modifying Effects of Nanosized Diamonds on Hydrolytic Potential of Macrophages In Vitro

et al. 2015

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The effects of nanosized particles (4-6 nm) of synthetic diamonds on mouse macrophage expression and secretion of lysosomal cathepsins (B and D) and MMP-1 and MMP-9 were studied in vitro. Culturing of peritoneal macrophages in medium with diamond nanoparticles led to an increase in the counts of macrophages expressing the above enzymes and to stimulation of their secretion. However, the manifestations of these effects varied significantly for various enzymes. The data indicate modulation of macrophage functions by nanodiamonds. These results help better understand the possible role of the "corpuscular" xenobiotic factors in the pathogenesis of diseases associated with macrophage capturing of these factors irrespective of their chemical "activity".

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Effect of Preliminary Load of Macrophages with Silicium Dioxide on Phagocytosis of BCG Strain Micobacteria by Macrophages and Antimicrobial Activity

Cited by 8 publications

References 7 publications

Acute Exposure to Crystalline Silica Reduces Macrophage Activation in Response to Bacterial Lipoproteins

Acute Exposure to Crystalline Silica Reduces Macrophage Activation in Response to Bacterial Lipoproteins

Establishment of rat model of silicotuberculosis and its pathological characteristic

Modifying Effects of Nanosized Diamonds on Hydrolytic Potential of Macrophages In Vitro

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