Effect of preconditioning in the liver against ischemia/reperfusion injury, protection of sinusoidal cells and alterations of gene transcription

Saito, Takuro; Ishii, Shunichi; Abe, Tsuyoshi; Tsuchiya, Takao; Kanno, Hiroki; Miyazawa, Masatsugu; Suzuki, Masao; Gotoh, M

doi:10.1016/s0041-1345(00)02345-9

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…It is well known that hepatic ischemia leads to cell death, either by necrosis or apoptosis, and that the survival of a postischemic liver depends on factors that limit necrosis and/or apoptosis. Previous work on the liver16, 17 and on other tissues18–20 has demonstrated that preconditioning induces a gene program of cell survival. This means hepatic ischemia and reperfusion elicit antagonistic forces, directing the hepatocytes toward either death or survival.…”

Section: Discussionmentioning

confidence: 99%

Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis

et al. 2006

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Hepatic ischemia-reperfusion injury associated with liver transplantation is an as yet unresolved problem in clinical practice. Preconditioning protects the liver against the deleterious effects of ischemia, although the mechanism underlying this preconditioning is still unclear. To profile gene expression patterns involved in hepatic ischemic preconditioning, we analyzed the changes in gene expression in rat livers by DNA microarray analysis. Approximately 116 genes were found to have altered gene expression after 8 hours of cold ischemia. Moreover, the expression of 218 genes was modified by classic preconditioning followed by the same ischemia process. Given the importance of the effects of ischemic preconditioning (IP) in minimizing the liver damage induced by sustained ischemia before reperfusion, this study analyzed the putative genes involved in the beneficial role of IP in liver grafts undergoing cold ischemia before its implantation in the recipient (IPϩI). Great differences were found in the gene expression pattern of ischemic preconditioning ϩ long cold ischemia (IPϩI) group when compared with the long cold ischemia alone condition (I), which could explain the protective regulatory mechanisms that take place after preconditioning. Twenty-six genes that were downregulated in cold ischemia were found upregulated after preconditioning preceding a long cold ischemia period. These would be genes activated or maintained by preconditioning. Heat shock protein genes and 3-hydroxy-3-methylglutaryl-coenzyme A reductase are among the most markedly induced transcripts.

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Section: Discussionmentioning

confidence: 99%

Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis

et al. 2006

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“…Similarly Yoshizumi et al [12]have demonstrated improved survival and increased tissue ATP with preconditioning in a rat liver resection model. Subsequently the IPC effect in the liver has been reproduced in several in vivo rodent models of partial and global liver ischemia [13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38]. These studies have demonstrated that liver IPC for warm ischemia resulted in decreased hepatocellular injury [17, 18], increased tissue ATP [12, 22], decreased tumor necrosis factor-α (TNF-α) [19, 31]and IL-6 [31]release, decreased leukocyte/endothelial cell interactions [25], decreased endothelial cell injury [39], increased peripheral liver blood flow [24], increased microcirculation [40, 41], decreased hepatocellular apoptosis [27], preserved energy metabolism [21], increased hepatic intracellular oxygenation [42]and remote organ protection [23].…”

Section: Evidence That Ipc Occurs In the Livermentioning

confidence: 99%

Protection of the Liver by Ischemic Preconditioning: A Review of Mechanisms and Clinical Applications

Koti¹,

Seifalian²,

Davidson³

2003

Dig Surg

115

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Ischemic preconditioning refers to the endogenous mechanism of protection against a sustained ischemic insult following an initial, brief ischemic stimulus. Ischemia-reperfusion injury of the liver is a major cause of morbidity and mortality in liver surgery and transplantation and ischemic preconditioning is a promising strategy for improving the outcome of liver surgery. The preconditioning phenomenon was first described in a canine model of myocardial ischemia-reperfusion injury in 1986 and since then has been shown to exist in other organs including skeletal muscle, brain, kidneys, retina and liver. In the liver, the preconditioning effect has been demonstrated in rodents and a recent study has demonstrated human clinical benefits of preconditioning during hemihepatectomies. Ischemic preconditioning has been described as an adaptive response and although the precise mechanism of hepatoprotection from preconditioning is unknown it is likely to be a receptor-mediated process. Several hypotheses have been proposed and this review assesses possible mechanisms of ischemic preconditioning and its role in hepatic surgery and liver transplantation. The future lies in defining the mechanisms of the ischemic preconditioning effect to allow drug targeting to induce the preconditioning response.

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“…In the mid-1990s, this concept started to spread to liver surgery and has been a topic of intense research during recent years (202). Multiple studies in rodents have demonstrated that ischemic preconditioning (IPC) is effective in reducing IRI in healthy animals as well as in obese animals and those with fatty liver (183,(203)(204)(205)(206)(207)(208)(209)(210)(211)(212)(213).…”

Section: Ischemic Preconditioning (Ipc)mentioning

confidence: 99%

“…In the setting of prolonged (75 minutes) total hepatic ischemia, IPC reduces caspase 3 activity and apoptosis (209). Other studies have suggested that additional apoptosis-regulating genes, such as c-jun, are involved in the protective effect of IPC and that IPC reduces transcription and inhibits apoptosis (203,205,210,220). IL-6 is another possible mediator of the IPC effect; however, studies have reported diverging results, including reports of hepatoprotection when IL-6 is administered as well as reports of reduced levels of IL-6 after IPC compared with I/R alone (206,207).…”

Section: Ischemic Preconditioning (Ipc)mentioning

confidence: 99%

Methods to Reduce Liver Ischemia/Reperfusion Injury

Björnsson¹

2014

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Effect of preconditioning in the liver against ischemia/reperfusion injury, protection of sinusoidal cells and alterations of gene transcription

Cited by 6 publications

References 8 publications

Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis

Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis

Protection of the Liver by Ischemic Preconditioning: A Review of Mechanisms and Clinical Applications

Methods to Reduce Liver Ischemia/Reperfusion Injury

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