Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice

Demeestere, Isabelle; Delbaere, Anne; Gervy, Christine; Bergh, Marc Van den; Devreker, Fabienne; Englert, Yvon

doi:10.1093/humrep/17.8.2152

Cited by 65 publications

(67 citation statements)

References 41 publications

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“…Dome/cavity formation almost always accompanied the surviving oocytes after growth, except in the 2-μl microdrops, suggesting that an intimate relationship exists between cavity formation and the presence of the oocyte itself or oocyte-derived factor(s). Given that a similar dome-like structure has been documented for several mammals [28][29][30], this morphological alteration seems to be firmly programmed and almost inseparable from normal oocyte growth. However, a dome-like structure is not an absolute requirement for oocytes to complete their growth, as evidenced in the pioneering studies of Eppig et al [6,31], which showed that mouse oocytes can accomplish growth in vitro, both in size and function, without forming a dome-like structure.…”

Section: Discussionsupporting

confidence: 58%

Growth of Bovine Oocyte-Granulosa Cell Complexes Cultured Individually in Microdrops of Various Sizes

Hirao

Shimizu

Iga

et al. 2009

Journal of Reproduction and Development

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Abstract. In mammalian embryo culture, the embryo:medium volume ratio can substantially affect embryo developmental performance. In the present study, we tested the possibility of improving the growth of bovine oocytes by reducing the medium volume, from a typical volume used in mouse follicle culture to a minimum possible level. A total of 282 complexes, each containing a growing oocyte 87-100 μm in diameter, were individually placed in microdrops of 2, 5, 10 or 20 μl and cultured for 13 days in a modified TCM-199 supplemented with 4% polyvinylpyrrolidone (molecular weight: 360 kDa). Oocyte diameter was measured every other day to trace the growth of each oocyte. Half the medium was replaced every other day or every day, and comparison revealed that daily replacement was more favorable for culture of these microdrops. The highest survival rate, 95%, occurred in the 20-μl microdrops, where most oocytes continued to grow throughout the culture period. In comparison, in the 5-and 10-μl microdrops, more oocytes died, and growth slowed towards the end of culture. In the 2-μl microdrops, which had the highest death rate, growth virtually ceased after 9 days. The surviving oocytes were usually accompanied by a characteristic dome-like structure of the granulosa cell mass, except in the 2-μl microdrops. In conclusion, the 20-μl microdrops allowed oocyte growth at an acceptable level, and any further reduction of the volume only had a negative impact on oocytes. Key words: Bovine, Growth, In vitro, Microdrop, Oocyte-granulosa cell complex (J. Reprod. Dev. 55: [88][89][90][91][92][93] 2009) n the mammalian ovary, only a small proportion of oocytes enter the growth phase periodically, and even fewer of these, after having grown, undergo maturation. Meanwhile, numerous other oocytes degenerate before or at various stages of growth [1]. These 'redundant' but potentially precious oocytes can be utilised if they are provided with a suitable alternative environment well before their death in the ovary [2]. One approach to this is to culture the growing oocytes for a substantial period of time so that growth can be completed [3][4][5].In the mouse, several culture systems have been established for growing oocytes in which oocytes can develop into fairly normal gametes [6][7][8][9][10]. There are, however, only a few studies reporting derivation of embryos from bovine oocytes grown in vitro [11,12]. We have previously described a 2-week culture system for isolated oocytes with associated granulosa cells from early antral follicles [12]. The oocytes attained after the culture were on average smaller than those fully grown in the ovary [12]. Perhaps, as Telfer et al. [13] pointed out, various underlying factors may render it difficult for bovine oocytes to reach their potential maximum size in vitro. Further improvements of the culture system are therefore necessary.An essential element for successful oocyte growth is that the oocytes receive appropriate nutrients and molecular signals from attached cells [14,15]. More precisel...

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Section: Discussionsupporting

confidence: 58%

Growth of Bovine Oocyte-Granulosa Cell Complexes Cultured Individually in Microdrops of Various Sizes

Hirao

Shimizu

Iga

et al. 2009

Journal of Reproduction and Development

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“…20 To this end, fragments were cut with a pair of 12-cm straight-bladed (f/f) scissors, homogenized 40 times in 3 mL of medium, and incubated with collagenase type IV (200 IU/mL) at 37 C for 20 minutes, being mixed every 10 minutes. After that, 10% FCS was added to the medium to stop collagenase activity.…”

Section: Follicular Isolationmentioning

confidence: 99%

Short-Term Culture of Ovarian Cortical Strips From Capuchin Monkeys (Sapajus apella): A Morphological, Viability, and Molecular Study of Preantral Follicular Development In Vitro

et al. 2013

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The aim of this study was to evaluate whether an in vitro culture (IVC) medium containing either or not b-mercaptoethanol (BME), bone morphogenetic protein 4 (BMP4), or pregnant mare serum gonadotrophin (PMSG) could be able to promote the development of capuchin monkeys' preantral follicles enclosed in ovarian cortical strips. Follicular viability after IVC was similar to control (89.32%). Primordial follicle recruitment to primary stage was not reached with IVC, but the rate of secondary follicle formation was increased in the medium supplemented with BME, BMP4, and PMSG (44.86%) when compared to IVC control (9.20%). In the medium supplemented with BME, BMP4, and PMSG, contrary to other media, anti-müllerian hormone-messenger RNA (mRNA) expression in ovarian tissue was upregulated (3.4-fold), while that of growth differentiation factor-9 was maintained. The BMP4-mRNA expression, however, appeared downregulated in all cultured tissues. Our findings show a favorable effect of BME, BMP4, and PMSG on the in vitro development of secondary follicles from capuchin monkeys.

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“…In the murine model, there are some reports that preantral follicles have been grown to the antral phase in vitro (Qvist et al 1990, Nayudu & Osborn 1992, were matured to metaphase II after human chorionic gonadotropin (hCG) stimulation (Cortvrindt & Smitz 1998), were fertilized and developed into blastocysts (Cortvrindt et al 1996a, Demeestere et al 2002 and resulted in live births after embryo transfer (Spears et al 1994). In 2001, Newton and Illingworth reported the survival and in vitro growth of murine follicles after isolation from ovarian tissues cryopreserved by a slow freezing method.…”

Section: Introductionmentioning

confidence: 99%

In vitro follicular development of cryopreserved mouse ovarian tissue

Segino¹,

Ikeda²,

Hirahara³

et al. 2005

Reproduction

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In a previous report, we showed that follicles isolated from frozen/thawed mouse ovarian tissues reached the mature follicle stage on the 12th day of culture. However, the developmental ability was lower than that of fresh ovarian tissue. The purpose of this study was to define a culture system with some technical modification for preantral follicles isolated from frozen/thawed ovarian tissue and to confirm cell injury. Ovaries obtained from three-week-old female mice were cryopreserved by the rapid freezing method. Preantral follicles isolated from frozen/thawed ovarian tissues were cultured for 12 -16 days. The follicles were then stimulated with human chorionic gonadotropin. In vitro fertilization was performed on the released cumulus-oocyte complexes (COCs). Preantral follicle viability was assessed by supravital staining using Hoechst 33258. Using this stain cell death was found in part of the granulosa cells of a follicle obtained from frozen/thawed ovarian tissue. On the 14th and 16th days of culture, the diameters of follicles isolated from frozen/thawed ovaries were larger than on the 12th day of culture. The released COCs were fertilized and developed to the blastocyst stage in 15.8% (12/76) of the oocytes taken from the fresh group, and in 0% (0/73), 2.9% (2/69) and 19.1% (22/115) of the oocytes taken from the frozen/thawed group that had been cultured for 12, 14 and 16 days respectively. The preantral follicles isolated from frozen/thawed mouse ovarian tissues developed slowly compared with the freshly prepared preantral follicles. During prolonged culture from 12 to 16 days, these follicles obtained the potential to fertilize and develop to the blastocyst stage.

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Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice

Abstract: Enzymatic follicular isolation did not affect theca cell development. Follicular steroidogenesis was enhanced after enzymatic isolation but the developmental capacity of oocytes was comparable to that obtained after mechanical isolation.

Cited by 65 publications

References 41 publications

Growth of Bovine Oocyte-Granulosa Cell Complexes Cultured Individually in Microdrops of Various Sizes

Growth of Bovine Oocyte-Granulosa Cell Complexes Cultured Individually in Microdrops of Various Sizes

Short-Term Culture of Ovarian Cortical Strips From Capuchin Monkeys (Sapajus apella): A Morphological, Viability, and Molecular Study of Preantral Follicular Development In Vitro

In vitro follicular development of cryopreserved mouse ovarian tissue

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