Effect of PPM on Alkaloid Production in Narcissus Confusus Shoot-Clumps

Colque, R.; Viladomat, F.; Codina, Carles

doi:10.17660/actahortic.2001.560.51

Cited by 2 publications

(1 citation statement)

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“…The shoot-clumps were cultured in 6 cm diameter babyfood jars (Sigma Chemical Co.) containing 25 mL of MS liquid medium supplemented with 30 g/L of sucrose and 3 mg/L of benzyladenine (control medium), which was formerly considered to be optimal for alkaloid production [4]. 0.5 ml/L of PPM (Plant Preservative Mixture) were added in all the medium cultures in order to avoid contamination, and after having observed no negative influence on the accumulation of alkaloids [14].…”

Section: Methodsmentioning

confidence: 99%

Improved Production of Galanthamine and Related Alkaloids by Methyl Jasmonate inNarcissus confususShoot-Clumps

et al. 2004

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Liquid-shake cultured shoot-clumps of Narcisus confussus were treated with the commonly used biotic elicitors methyl jasmonate, arachidonic acid, chitosan and salicylic acid. The effects of these compounds on the growth of the explants, as well as on the amount of the alkaloids released to the liquid culture medium and accumulated in the tissues at the end of the experiment were studied. The obtained results showed that, in general, high doses of these compounds had a negative effect on the growth of the explants, particularly the salicylic acid. On the contrary, the addition of methyl jasmonate, mainly at 25 microM, promoted the release of galanthamine and other related alkaloids to the liquid medium in proportions of up to 300% in relation to the control explants, and also their accumulation in tissues. The other elicitors studied did not have any interesting effects on the production of these Amaryllidaceae-type alkaloids.

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Section: Methodsmentioning

confidence: 99%

Improved Production of Galanthamine and Related Alkaloids by Methyl Jasmonate inNarcissus confususShoot-Clumps

et al. 2004

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Production of Galanthamine by Leucojum aestivum Shoots Grown in Different Bioreactor Systems

Schumann¹,

Berkov

Claus³

et al. 2012

Appl Biochem Biotechnol

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The production of galanthamine by shoots of Leucojum aestivum grown in different bioreactor systems (shaking and nonshaking batch culture, temporary immersion system, bubble bioreactor, continuous and discontinuous gassing bioreactor) under different culture conditions was studied. The influence of the nutrient medium, weight of inoculum, and size of bioreactor on both growth and galanthamine production was studied. The maximal yield of galanthamine (19.416 mg) was achieved by cultivating the L. aestivum shoots (10 g of fresh inoculum) in a temporary immersion system in a 1-L bioreactor vessel which was used as an airlift culture vessel, gassing 12 times per day (5 min).

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Effect of PPM on Alkaloid Production in Narcissus Confusus Shoot-Clumps

Cited by 2 publications

References 0 publications

Improved Production of Galanthamine and Related Alkaloids by Methyl Jasmonate inNarcissus confususShoot-Clumps

Improved Production of Galanthamine and Related Alkaloids by Methyl Jasmonate inNarcissus confususShoot-Clumps

Production of Galanthamine by Leucojum aestivum Shoots Grown in Different Bioreactor Systems

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