Effect of post-storage filters vs. pre-storage filters for leukoreduction of blood components on clinical outcomes: a systematic review protocol

Silva, Natasha Dejigov Monteiro da; Nukui, Youko; Takahashi, Juliana; Cruz, Diná de Almeida Lopes Monteiro da; Nogueira, Lilia de Souza

doi:10.11124/jbies-20-00353

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…It was initially hypothesized that the reduction in LRF-CD34+ cells was correlated with the age, however the statistical analyses revealed that there was no relationship between the measured parameters in this study and the age of participants so that the loss of sensitivity to PB stimuli or any reduction in mobilizing cytokines might be the possible mechanisms of this decrease (28)(29)(30)(31)(32).…”

Section: Discussionmentioning

confidence: 82%

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

et al. 2022

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Scan to discover onlineBackground & Objective: Trapped cell population in leukoreduction filters (LRFs) contains such a significant number of CD34 + hematopoietic stem cells that can be recovered to be used in research studies.Methods: Samples (n=20) were obtained from 10 first-time donors and 10 regular blood donors with more than 30 times blood donation. After separating leukocytes from LRFs by backflushing, total leukocyte number and differential count were determined in both groups using an automated haemocytometer. Then cell viability and CD34 + cell quantification were assessed using 7-amino-actinomycin D and fluorescent-labeled monoclonal antibodies using flow cytometry, respectively.Results: Total leukocyte count was 665±164.92×10 6 in the first-time blood donors and 883±233.89×10 6 in the regular donors, which were not significantly different (P=0.08). While the number of CD34 + cells was significantly reduced in the regular donors compared to the first-time donors (0.58±0.20×10 6 /µL vs. 0.36±0.22×10 6 /µL; P=0.034). There was no significant difference in terms of absolute neutrophil count (10.58±3.66×0 6 vs. 13.17±6.45×10 6 /µL; P=0.349), lymphocytes (7.75±3.11×10 6 vs. 10.38±3.77×10 6 /µL; P=0.917), and monocytes (2.31±0.88×10 6 vs. 2.59±1.09×10 6 /µL; P=0.591) between the first-time and regular donor groups, respectively. Based on the correlation coefficients, the participants' age had no significant effect on these variables. Conclusion:The results of this study depicted that regular blood donation reduces the number of CD34 + cells in the peripheral blood (PB) of regular donors while it has no significant effect on the ratio of myeloid to lymphoid cells of the two groups.

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Section: Discussionmentioning

confidence: 82%

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

et al. 2022

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“…This systematic review had the protocol published a priori 22 and registered in the PROSPERO database (CRD42020192202). Considering that the post-storage lter (bedside) has been the oldest and most widely used technology for decades, the PICO structure presented in the review protocol 22 was changed with regard to the intervention and the comparator by consensus among the authors of this review. Thus, the prestorage lter (bench or inline) was considered for intervention, and the post-storage lter (bedside) was considered for the comparator.…”

Section: Methodsmentioning

confidence: 99%

Effect of post-storage filters vs. pre-storage filters for leukoreduction of blood components on clinical outcomes: A systematic review

Silva,

Nukui,

Takahashi

et al. 2024

Preprint

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Background: Leukoreduction has been used to limit the risk of adverse events. The most commonly used methodology is filtration (pre or post-storage). However, whether pre-storage filtration is better than post-storage filtration needs to be clearly defined, particularly for countries that still use post-storage filtration. This study aimed to synthesize the best available evidence on the effectiveness of pre-storage filters compared with post-storage filters for transfusion reactions, for the occurrence of infections, for the length of hospital stay, and for the death of patients undergoing leukoreduced transfusion. Methods: Eight databases and gray literature were searched, and GRADE was used to determine the certainty of the evidence. Results: The meta-analysis showed that pre-storage filtration was a protective factor against febrile non-hemolytic transfusion reactions in red blood cells and platelet concentrate transfusions. The same did not occur for post-surgical infection after platelet concentrate transfusions. Only one study analyzed the length of hospital stay and showed no significant difference between patients who received leukoreduced transfusions according to the type of filter used. According to the GRADE criteria, the certainty of the evidence for febrile non-hemolytic transfusion reactions was low for red blood cells and very low for platelet concentrate due to the high risk of bias. For infection was low risk due to imprecision. Conclusions: The results of this review showed that the certainty of recommending the best type of filter (pre or post-storage) for the benefit of the outcomes analyzed is still fragile; therefore, more robust evidence is needed. Systematic review registration number: PROSPERO CRD42020192202

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Effect of post-storage filters vs. pre-storage filters for leukoreduction of blood components on clinical outcomes: a systematic review and meta-analysis

Dejigov Monteiro da Silva,

Nukui,

Takahashi

et al. 2024

Syst Rev

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Effect of post-storage filters vs. pre-storage filters for leukoreduction of blood components on clinical outcomes: a systematic review protocol

Cited by 4 publications

References 19 publications

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

Effect of post-storage filters vs. pre-storage filters for leukoreduction of blood components on clinical outcomes: A systematic review

Effect of post-storage filters vs. pre-storage filters for leukoreduction of blood components on clinical outcomes: a systematic review and meta-analysis

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