Effect of Pore Size of MgO-templated Carbon on the Direct Electrochemistry of <small>D</small>-fructose Dehydrogenase

Abstract

MgO-templated porous carbon (MgOC) was developed for D-fructose dehydrogenase (FDH) electrodes. MgOCs with an average pore diameter ranging from 10 to 100 nm were used in this study. FDH adsorbed on a MgOC electrode exhibited significant catalytic currents for D-fructose-oxidation without a redox mediator. When the pore size of MgOC was much larger than the size of FDH, a sufficient amount of FDH was adsorbed in the mesopore on and even inside the MgOC structure. In contrast, when the pore size of MgOC was comparable to the size of FDH, the catalytic current depended only on the amount of enzyme adsorbed in mesopores formed at the surface of the carbon particles; however, an enhanced thermal stability of FDH was observed. Thus, FDH was stabilized through encaging in carbon mesopores with a size comparable to that of the enzyme.

Direct Electron Transfer-Type Bioelectrocatalysis of Redox Enzymes at Nanostructured Electrodes

“…MgO-templated carbons with various pore sizes were investigated as scaffolds of a DET-type bioelectrocatalysis of FDH [33]. The sizes of the mesopores studied were in the range from 10 to 100 nm.…”

Impact of Carbon Nanotube Surface Chemistry on Hydrogen Oxidation by Membrane‐Bound Oxygen‐Tolerant Hydrogenases

“…Enzymes often show improved stability upon immobilization on a solid support . For instance, enhanced thermostability was recently reported for fructose dehydrogenase inserted into a mesoporous carbon template . The Re MbH dispersed in a water‐in‐oil microemulsion was shown to be more thermostable …”

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

“…In recent years, various electrode structures have been reported as being scaffolds suitable for DET reactions. [1][2][3][4][5][6][7] For this purpose, carbon particles, carbon nanotubes, [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] and 3D gold nanostructures [28][29][30][31][32][33][34] have been frequently used because of the curvature effect of their structures to increases the probability of orientations suitable for DET reactions 26,27 and the facile surface functionalization with thiols as well as high surface-to-volume ratios of the structures. However, it is difficult to precisely control the electrode shape of nanostructured materials.…”