Effect of poly(vinyl alcohol) treatment of the dyematrix on the chromatography of pyruvate kinase

Garg, Nandita; Galev, Igor Yu.; Mattìasson, Bo

doi:10.1007/bf00241691

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Cited by 8 publications

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“…a shielding polymer is needed. Recent developments in Shielded Affinity Chromatography have demonstrated the effectiveness of using PVA to prevent the stron g binding of proteins OII gel matrix (Grag et al, 1994). Therefore, exploration of the utility of PVA in MFE process using MF membrane spaced electrolyzer was promising.…”

Section: Introductionmentioning

confidence: 99%

Continuous separation of proteins by poly(vinyl alcohol) shielded Multichannel Flow Electrophoresis

et al. 1996

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A hydrophilic polysulphone membrane spaced multicompartment electrolyzer for Multichannel Flow Electrophoresis (MFE) was developed. Application of poly(vinyl alcohol) (PVA) in MFE resulted in a 35% increase of protein transmembrane flux. Continuous separation of bovine serum albumin (BSA) and haemoglobin bovine blood (HBB) model mixture by PVA shielded MFE yielded 56mg BSA and 48mg HBB per hour. The average recovery was 65%.

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Section: Introductionmentioning

confidence: 99%

Continuous separation of proteins by poly(vinyl alcohol) shielded Multichannel Flow Electrophoresis

et al. 1996

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Polymer-shielded dye-affinity chromatography

1996

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Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.

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