Effect of Poloxamer 407 on the Adherence of Pseudomonas aeruginosa to Corneal Epithelial Cells

Portolés, Marta; Austin, F E; Nos-Barbera, S.; Paterson, Christopher A.; Mf, Refojo

doi:10.1097/00003226-199501000-00010

Cited by 2 publications

(3 citation statements)

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“…The prolonged lower concentrations would protect the prosthetic device against endogenous infection. Moreover, antibiotic activity should be reinforced by the non specific antiadhesive effect of P407 that can limit protein and bacterial adhesion 8, 10. As the gels remain at the injection site, the duration of active vancomycin levels is determined by the dilution rate and diffusion through extra‐micellar aqueous channels, and by the biological degradation of the gel matrix itself 27…”

Section: Resultsmentioning

confidence: 99%

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In situ gel forming systems of poloxamer 407 and hydroxypropyl cellulose or hydroxypropyl methyl cellulose mixtures for controlled delivery of vancomycin

Talasaz¹,

Ghahremankhani²,

Moghadam³

et al. 2008

J of Applied Polymer Sci

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Poloxamers are a family of triblock copolymers consisting of two hydrophilic blocks of polyoxyethylene separated by a hydrophobic block of polyoxypropylene, which form micelles at low concentrations and form clear thermally reversible gels at high concentrations. The objective of this study was to develop an in situ gel forming drug delivery system for vancomycin using the minimum possible ratio of poloxamer 407 (P407). Decreasing the concentration of poloxamer could reduce the risk of hypertriglyceridemia induction. Different additives were added to the poloxamer formulations. It was observed that among different additives, hydroxypropyl methyl cellulose (HPMC) and hydroxypropyl cellulose (HPC) can decrease poloxamer concentration required to form in situ gelation from 18 to 10%. The dynamic viscoelastic properties of the samples were determined. Both the storage modulus and the loss modulus of the samples increased abruptly as the temperature passed a certain point. The gelling temperature was in the order of P407 : HPC (10 : 10 w/w) < P407 : HPMC (10 : 10 w/w) < P407 : HPMC (15 : 5 w/w) < P407 : HPC (15 : 5 w/w). Drug release rate could be controlled by changing the type and ratio of additives as well as the amount of drug loaded. It can be concluded that combining P407 and cellulose derivatives could be a promising strategy for preparation of thermally reversible in situ gel forming delivery systems with low poloxamer concentration.

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Section: Resultsmentioning

confidence: 99%

“…It has been used for topical, transdermal, ophthalmic, and implantable applications 3–7. In addition, P407 exhibits non specific antiadhesive properties that can prevent the adherence of proteins and bacteria, and thereby hinder infections 8–10…”

Section: Introductionmentioning

confidence: 99%

In situ gel forming systems of poloxamer 407 and hydroxypropyl cellulose or hydroxypropyl methyl cellulose mixtures for controlled delivery of vancomycin

Talasaz¹,

Ghahremankhani²,

Moghadam³

et al. 2008

J of Applied Polymer Sci

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“…4 5 8 Microsphere concentrations of 0.05%, 0.03%, 0.02%, and 0.01% and water only were prepared under sterile conditions (surfactant poloxamer 407 was included to prevent coagulation). 15 The stability of the light scatter model was determined by assessing HRF measurements against a plain white paper background for the 0.05% cell which had not been disturbed for 24 hours, and then after vigorous shaking. The mean (SD) value (three repeats) for 10 6 10 pixels was 47 735 (373) arbitrary units (au), and immediately after shaking it was not significantly different (47 335 (431) au).…”

Section: Simulated Light Scatter Modelmentioning

confidence: 99%

Impact of simulated light scatter on scanning laser Doppler flowmetry

Venkataraman

Hudson²,

Harvey³

et al. 2005

British Journal of Ophthalmology

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Aim: To determine the impact of simulated light scatter on scanning laser Doppler flowmetry (SLDF) assessment of retinal capillary blood flow and retinal image quality. Methods: One eye of 10 normal subjects (mean (SD) age 24 (1.7) years, range 22-27) was randomly selected. Varying concentrations of polystyrene microspheres were suspended in optically clear cells to simulate light scatter. The microsphere concentrations used were 0.05%, 0.03%, 0.02%, 0.01%, and a cell containing only water. LogMAR visual acuity and contrast sensitivity were measured both with and without cells. Optimal focus and alignment was established by acquiring three SLDF images each of the optic nerve head (ONH) and of the macula using the Heidelberg retina flowmeter (HRF) with no cell in place. SLDF images were subsequently acquired with each of the light scatter cells mounted in front of the HRF. The group mean retinal capillary blood flow was compared using repeated measures analysis of variance (reANOVA) as a function of microsphere concentration. Results: Retinal capillary blood flow increased significantly in the ONH, nasal macula, fovea, and temporal macula with increasing microsphere concentration (p,0.0001). Using Dunnett's post hoc test, retinal capillary blood flow was found to be significantly increased relative to the no cell condition for the 0.03% and 0.05% cell concentrations. Conclusions: Simulated light scatter produces an artifactual increase in retinal capillary blood flow. The impact of cataract on SLDF measurements has yet to be determined.

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Effect of Poloxamer 407 on the Adherence of Pseudomonas aeruginosa to Corneal Epithelial Cells

Cited by 2 publications

References 0 publications

In situ gel forming systems of poloxamer 407 and hydroxypropyl cellulose or hydroxypropyl methyl cellulose mixtures for controlled delivery of vancomycin

In situ gel forming systems of poloxamer 407 and hydroxypropyl cellulose or hydroxypropyl methyl cellulose mixtures for controlled delivery of vancomycin

Impact of simulated light scatter on scanning laser Doppler flowmetry

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