Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine

Dauelsberg, Patricia; Matus, José Tomás; Poupin, María Josefina; Leiva-Ampuero, Andrés; Godoy, Francisca; Vega, Andrea; Arce‐Johnson, Patricio

doi:10.1016/j.jplph.2011.03.006

Cited by 28 publications

(31 citation statements)

References 51 publications

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“…Auxin biosynthesis was verified by analyzing the expression patterns of the VvASB1-like , VvYUC2 , and VvYUC6 genes, which encode key enzymes in auxin biosynthesis. The expression of VvASB1-like was reported by Dauelsberg et al [36], and VvYUC2 and VvYUC6 were found to be the closest Vitis homologue genes of Arabidopsis YUC2 ( AtYUC2 ) and AtYUC6 , and the tomato YUC2 homologue, ToFZY2 , which were abundantly expressed in flowers [43], [48]. The VvYUC2 and VvYUC6 proteins shared more than 65% identity and had highly conserved FAD and NADPH binding sites with those of Arabidopsis and tomato (Figure S3).…”

Section: Resultsmentioning

confidence: 69%

“…Primers used in previous studies [36], [53] were used for the grapevine auxin biosynthesis gene, the putative Vitis anthranilate synthase β subunit-like ( VvASB1-like ), and two auxin signaling Vitis Gretchen Hagen3 ( GH3 ) family genes. qRT-PCR was performed with the SYBR Premix Ex Taq (Takara) on a Thermal Cycler Dice Real-Time System TP800, version 4.0 (Takara), under universal thermal cycling conditions described by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

“…The effects of GA application have been studied on early ripening and berry enlargement [32]–[34], and on the appropriate application timing at the pre-bloom stage for the induction of seedless grapes [31], [35]. On a molecular level, both GA and auxin biosynthesis genes are up-regulated after pollination in grapevines [3], [36], [37]. However, although GA-mediated parthenocarpy is a highly desirable trait for table grapes, how GA induces parthenocarpic fruit development remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Jung

Hur

et al. 2014

PLoS ONE

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Fruit set is initiated only after fertilization and is tightly regulated primarily by gibberellins (GAs) and auxins. The application of either of these hormones induces parthenocarpy, fruit set without fertilization, but the molecular mechanism underlying this induction is poorly understood. In the present study, we have shown that the parthenocarpic fruits induced by GA application at pre-bloom result from the interaction of GA with auxin signaling. The transcriptional levels of the putative negative regulators of fruit set initiation, including Vitis auxin/indole-3-acetic acid transcription factor 9 (VvIAA9), Vitis auxin response factor 7 (VvARF7), and VvARF8 were monitored during inflorescence development in seeded diploid ‘Tamnara’ grapevines with or without GA application. Without GA application, VvIAA9, VvARF7, and VvARF8 were expressed at a relatively high level before full bloom, but decreased thereafter following pollination. After GA application at 14 days before full bloom (DBF); however, the expression levels of VvIAA9 and VvARF7 declined at 5 DBF prior to pollination. The effects of GA application on auxin levels or auxin signaling were also analyzed by monitoring the expression patterns of auxin biosynthesis genes and auxin-responsive genes with or without GA application. Transcription levels of the auxin biosynthesis genes Vitis anthranilate synthase β subunit (VvASB1-like), Vitis YUCCA2 (VvYUC2), and VvYUC6 were not significantly changed by GA application. However, the expressions of Vitis Gretchen Hagen3.2 (VvGH3.2) and VvGH3.3, auxin-responsive genes, were up-regulated from 2 DBF to full bloom with GA application. Furthermore, the Vitis GA signaling gene, VvDELLA was up-regulated by GA application during 12 DBF to 7 DBF, prior to down-regulation of VvIAA9 and VvARF7. These results suggest that VvIAA9 and VvARF7 are negative regulators of fruit set initiation in grapevines, and GA signaling is integrated with auxin signaling via VvDELLA during parthenocarpic fruit development in grapevines.

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Section: Resultsmentioning

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Jung

Hur

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

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“…Amplification was performed under the following conditions: 95°C for 10 min, followed by 40 cycles of 94°C, 30 s; 60-64°C, 30 s; and 72°C, 30 s, followed by a melting cycle from 55°C to 95°C. Relative gene expression calculations were conducted as described in the software manufacturer’s instructions: an accurate ratio between the expression of the gene of interest (GOI) and the housekeeping (HK) gene was calculated according to equation: 2- (∆CtGOI-HK) [79]. Then, gene expression levels were normalized to the average value of the treatment with less expression.…”

Section: Methodsmentioning

confidence: 99%

Effects of the Plant Growth-Promoting Bacterium Burkholderia phytofirmans PsJN throughout the Life Cycle of Arabidopsis thaliana

et al. 2013

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Plant growth-promoting rhizobacteria (PGPR) induce positive effects in plants, such as increased growth or reduced stress susceptibility. The mechanisms behind PGPR/plant interaction are poorly understood, as most studies have described short-term responses on plants and only a few studies have analyzed plant molecular responses under PGPR colonization. Here, we studied the effects of the PGPR bacterial model Burkholderia phytofirmans PsJN on the whole life cycle of Arabidopsis thaliana plants. We reported that at different plant developmental points, strain PsJN can be found in the rhizosphere and also colonizing their internal tissues. In early ontogeny, strain PsJN increased several growth parameters and accelerated growth rate of the plants. Also, an Arabidopsis transcriptome analysis revealed that 408 genes showed differential expression in PsJN-inoculated plants; some of these genes are involved in stress response and hormone pathways. Specifically, genes implicated in auxin and gibberellin pathways were induced. Quantitative transcriptional analyses of selected genes in different developmental stages revealed that the beginning of these changes could be evidenced early in development, especially among the down-regulated genes. The inoculation with heat-killed bacteria provoked a more severe transcriptional response in plants, but was not able to induce plant growth-promotion. Later in ontogeny, the growth rates of inoculated plants decreased with respect to the non-inoculated group and, interestingly, the inoculation accelerated the flowering time and the appearance of senescence signs in plants; these modifications correlate with the early up-regulation of flowering control genes. Then, we show that a single inoculation with a PGPR could affect the whole life cycle of a plant, accelerating its growth rate and shortening its vegetative period, both effects relevant for most crops. Thus, these findings provide novel and interesting aspects of these relevant biological interactions.

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“…Real time RT-qPCR was performed using a MX3000P detection system (Stratagene), as previously described [52], and the SensiMix Plus SYBR commercial kit (Quantace) was utilized, according to the manufacturer's instructions. A fragment of the grapevine VvGPDH gene ( VvGLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE , GI: 359491598) [53] and a fragment of the grapevine VvUBI1 gene ( VvUbiquitin1 , TC53702, TIGR database) [54] were utilized as housekeepings genes.…”

Section: Methodsmentioning

confidence: 99%

Differential Behavior within a Grapevine Cluster: Decreased Ethylene-Related Gene Expression Dependent on Auxin Transport Is Correlated with Low Abscission of First Developed Berries

et al. 2014

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In grapevine, fruit abscission is known to occur within the first two to three weeks after flowering, but the reason why some berries in a cluster persist and others abscise is not yet understood. Ethylene sensitivity modulates abscission in several fruit species, based on a mechanism where continuous polar auxin transport across the pedicel results in a decrease in ethylene perception, which prevents abscission. In grapevine, flowering takes about four to seven days in a single cluster, thus while some flowers are developing into berries, others are just starting to open. So, in this work it was assessed whether uneven flowering accounted for differences in berry abscission dependent on polar auxin transport and ethylene-related gene expression. For this, flowers that opened in a cluster were tagged daily, which allowed to separately analyze berries, regarding their ability to persist. It was found that berries derived from flowers that opened the day that flowering started – named as “first berries” – had lower abscission rate than berries derived from flowers that opened during the following days – named as “late berries”. Use of radiolabeled auxin showed that “first berries” had higher polar auxin transport, correlated with lower ethylene content and lower ethylene-related transcript abundance than “late berries”. When “first berries” were treated with a polar auxin transport inhibitor they showed higher ethylene-related transcript abundance and were more prone to abscise than control berries. This study provides new insights on fruit abscission control. Our results indicate that polar auxin transport sustains the ability of “first berries” to persist in the cluster during grapevine abscission and also suggest that this could be associated with changes in ethylene-related gene expression.

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Effect of pollination and fertilization on the expression of genes related to floral transition, hormone synthesis and berry development in grapevine

Cited by 28 publications

References 51 publications

Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Gibberellin Application at Pre-Bloom in Grapevines Down-Regulates the Expressions of VvIAA9 and VvARF7, Negative Regulators of Fruit Set Initiation, during Parthenocarpic Fruit Development

Effects of the Plant Growth-Promoting Bacterium Burkholderia phytofirmans PsJN throughout the Life Cycle of Arabidopsis thaliana

Differential Behavior within a Grapevine Cluster: Decreased Ethylene-Related Gene Expression Dependent on Auxin Transport Is Correlated with Low Abscission of First Developed Berries

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