Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

Wu, Baojiang; Xue, Hong-yan; Chen, Liping; Dai, Yanfeng; Guo, Jitong; Li, Xihe

doi:10.1016/s2095-3119(13)60485-2

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…It is surprising to observe that increasing dose of eCG had lower prolificacy rate than L-eCG, suggesting that the high concentration of eCG could be beneficial for follicular dynamics but detrimental to early or late embryo development (Randi et al, 2018). In mouse, the embryonic quality (inner cell mass to trophectoderm ratio) was negatively affected when high dose of gonadotropins (eCG or hCG) was used for the ovarian stimulation (Wu et al, 2013). Similarly, Lin et al (2021) reported negative effects of high eCG dose on fertilization rate and embryo development including the formation of 4-cell embryos and blastocysts, concurrent with an increased risk of chromosome aneuploidy.…”

Section: Discussionmentioning

confidence: 99%

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Sharif

Hassan

Arshad

et al. 2022

Reprod Domestic Animals

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The objectives of the experiment were to determine the effect of two doses of equine chorionic gonadotropin (eCG) in a standard synchronization protocol based on a short‐term progesterone (P4) priming on ovarian structures and haemodynamics, concentrations of steroid hormones and prolificacy rate when oestrus was induced during low‐breeding season (LBS) in Beetal dairy goats. We hypothesized that inclusion of eCG in a short‐term P4 priming‐based synchronization protocol would increase the blood perfusion to ovarian structures leading to enhance oestrous and ovulatory responses and prolificacy rate in goats. Forty‐two multiparous acyclic goats were blocked by body condition and, within block, assigned randomly to receive saline as control (CON), low eCG (L‐eCG; 300 IU) or high eCG (H‐eCG; 600 IU) dose. Initially, a controlled internal drug release (CIDR) device was placed in the anterior vagina on d −8, followed by removal of CIDR on d −3, concurrent with the administration of PGF2α and eCG according to their respective treatments. Goats were monitored for oestrous response. B‐mode and Doppler ultrasonography was performed with 12‐h interval, starting from day −3 until natural breeding (day 0), and then on days 5, 10, 15 and 20 post‐breeding to monitor follicular and luteal dynamics and blood flow, respectively. Blood was sampled at 0, 12, 24, 36 and 60 h after CIDR removal to quantify plasma concentrations of estradiol‐17β (E2), whereas plasma concentrations of P4 were assayed at days 5, 10, 15 and 20 after breeding. Pregnancy and prolificacy rates were determined at day 30 and 150 after breeding, respectively. Data were analysed with mixed‐effects models, and orthogonal contrasts were used to evaluate the effect of treatment [Con vs. (½ L‐eCG + ½ H‐eCG)] and dose of eCG (L‐eCG vs. H‐eCG). Data are presented in sequence as CON, L‐eCG, H‐eCG (LSM ± SEM). The oestrous intensity score (152.9 vs. 182.7 vs. 186.5 ± 15.1; p = .02) was greater in eCG‐treated goats as compared to CON. Administration of eCG reduced the intervals to standing oestrus (66.2 vs. 41.8 vs. 48.9 h ± 5.5; p = .05), breeding (70.2 vs. 44.4 vs. 45.4 h ± 4.5; p = .03) and ovulation (84.5 vs. 61.2 vs. 63.4 h ± 6.2; p = .05) compared with CON goats. The mean growth rate of pre‐ovulatory follicle was greater (1.11 vs. 1.49 vs. 1.45 mm ± 0.08; p = .01) in eCG‐treated goats resulting in an increased diameter of pre‐ovulatory follicle (6.27 vs. 7.20 vs. 7.31 mm ± 0.07; p < .01) and corpora lutea (6.75 vs. 8.26 vs. 8.07 mm ± 0.42; p = .04) than CON. The mean follicular blood flow did not differ among treatments; however, the mean luteal blood flow was greater in L‐eCG‐treated goats (0.81 vs. 1.61 vs. 1.07 cm2 ± 0.12; p = .001). The mean concentrations of E2 (4.03 vs. 5.21 vs. 4.78 pg/ml ± 0.42; p = .04) and P4 (4.85 vs. 6.39 vs. 6.22 ng/ml ± 0.34; p = .04) were greater in eCG‐treated goats. The twinning rate did not differ between treatments; nevertheless, prolificacy rate was greater (p = .04) in L‐eCG‐treated goats. Collectively, our data sugges...

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Section: Discussionmentioning

confidence: 99%

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Sharif

Hassan

Arshad

et al. 2022

Reprod Domestic Animals

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“…Indeed, utilization of higher doses led to more abortions and did not cause any increase innumber of live fetuses. Some studies have concluded that overdose can lead to pre-implantation abortion ( 24 ). The mean of live fetuses’ weight and height in 15 Iu group was less than that of 2Gy + 10 Iu group ( Table I ).…”

Section: Discussionmentioning

confidence: 99%

Effects of gamma radiation on fetal development in mice

Dehghan

Mozdarani

Khoradmehr

et al. 2016

IJRM

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Background:Many cancer patients receive radiotherapy which may lead to serious damages to the ovary storage and the matrix muscle state. Some of these patients may admit to infertility clinics for having pregnancy and on the other hand hormonal administration for superovulation induction is a routine procedure in assisted reproduction technology (ART) clinics. Objective:This study aimed to investigate fertility and fetuses of hormone treated super ovulated female mice who had received whole-body gamma irradiation before mating.Materials and Methods:Female mice were randomly categorized into a control group and 3 experimental groups including: Group I (Irradiation), Group II (Superovulation), and Group III (Superovulation and Irradiation). In hormone treated groups, mice were injected with different doses of pregnant mare's serum gonadotropin (PMSG) followed with human chorionic gonadotropin (HCG). Irradiation was done using a Co-60 gamma ray generator with doses of 2 and 4 Gy. Number of fetuses counted and the fetus’s weight, head circumference, birth height, the number of live healthy fetuses, the number of fetuses with detected anomalies in the body, the sum of resorption and arrested fetuses were all recorded as outcome of treatments.Results:In the group I and group II, increased radiation and hormone dose led to a decrease in the number of survived fetuses (45 in 2 Gy vs. 29 in 4 Gy for irradiated group) as well as from 76 in 10 units into 48 in 15 units. In the group III, a higher dose of hormone in the presence of a 2 Gy irradiation boosted the slink rate; i.e. the number of aborted fetuses reached 21 cases while applying the dose of 15 Iu, whereas 6 cases of abortion were reported applying the hormone with a lower dose. Among different parameters studied, there was a significant difference in parameters of weight and height in the mouse fetuses (p=0.01). Conclusion:The data indicated that use of ovarian stimulating hormones in mice that received pre mating gamma irradiation did not significantly increase the pregnancy rates.

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“…Superovulation using equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (HCG) in place of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) is a widely used protocol for maximizing the numbers of oocytes and preimplantation embryos obtained from animals, respectively (Uysal et al, 2018 ). Results from animal studies have shown that gonadotropin stimulation can reduce the developmental potential of embryos in vitro (Karagenc et al, 2004 ; Wu et al, 2013 ; Swann, 2014 ), cause mitochondrial dysfunction in mouse oocytes (Ge et al, 2012 ), alter DNA methyltransferase protein expression (Uysal et al, 2018 ), affect the translational control of maternally stored messenger RNA (mRNA) (Ozturk et al, 2016 ) in early embryos, impair embryo quality and the uterine milieu (Ertzeid and Storeng, 2001 ), and change the appearance of imprinted genes in the midgestation mouse placenta (Fortier et al, 2008 ), resulting in delayed development (Ertzeid and Storeng, 1992 ; Ingrid and Thomas, 2001 ) or embryo death (Beaumont and Smith, 1975 ) and leading to an increased rate of offspring malformations (Sakai and Endo, 1987 ). Moreover, anti-eCG serum can effectively reduce the negative effect of eCG on the normal ovulation of mice (Lin et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

High Dosages of Equine Chorionic Gonadotropin Exert Adverse Effects on the Developmental Competence of IVF-Derived Mouse Embryos and Cause Oxidative Stress-Induced Aneuploidy

Lin

Huang

et al. 2021

Front. Cell Dev. Biol.

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Gonadotropins play vital roles in the regulation of female reproductive ability and fertility. Our study aimed to determine the effects of superovulation induced by increasing doses of equine chorionic gonadotropin [eCG; also referred to as pregnant mare serum gonadotropin (PMSG)] on the developmental competence of mouse embryos and on aneuploidy formation during in vitro fertilization (IVF). eCG dose-dependently enhanced the oocyte yield from each mouse. Administration of 15 IU eCG significantly reduced the fertilization rate and the formation of four-cell embryos and blastocysts and increased the risk of chromosome aneuploidy. The IVF-derived blastocysts in the 15 IU eCG treatment group had the fewest total cells, inner cell mass (ICM) cells and trophectoderm (TE) cells. Moreover, more blastocysts and fewer apoptotic cells were observed in the 0, 5, and 10 IU eCG treatment groups than in the 15 IU eCG treatment group. We also investigated reactive oxygen species (ROS) levels and variations in several variables: mitochondrial membrane potential (MMP); active mitochondria; mitochondrial superoxide production; adenosine triphosphate (ATP) content; spindle structures; chromosome karyotypes; microfilament distribution; and the expression of Aurora B [an important component of the chromosomal passenger complex (CPC)], the spindle assembly checkpoint (SAC) protein mitotic arrest deficient 2 like 1 (MAD2L1), and the DNA damage response (DDR) protein γH2AX. Injection of 15 IU eCG increased ROS levels, rapidly reduced MMP, increased active mitochondria numbers and mitochondrial superoxide production, reduced ATP content, increased abnormal spindle formation rates, and induced abnormalities in chromosome number and microfilament distribution, suggesting that a high dose of eCG might alter developmental competence and exert negative effects on IVF-obtained mouse embryos. Additionally, the appearance of γH2AX and the significantly increased expression of Aurora B and MAD2L1 suggested that administration of relatively high doses of eCG caused Aurora B-mediated SAC activation triggered by ROS-induced DNA damage in early mouse IVF-derived embryos for self-correction of aneuploidy formation. These findings improve our understanding of the application of gonadotropins and provide a theoretical basis for gonadotropin treatment.

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Effect of PMSG/hCG Superovulation on Mouse Embryonic Development

Cited by 9 publications

References 25 publications

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Effects of gamma radiation on fetal development in mice

High Dosages of Equine Chorionic Gonadotropin Exert Adverse Effects on the Developmental Competence of IVF-Derived Mouse Embryos and Cause Oxidative Stress-Induced Aneuploidy

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