Effect of Ploidy Level on Growth, Differentiation, and Morphology in Physcomitrella patens

Schween, Gabriele; Schulte, Julia; Reski, Ralf; Hohe, Annette

doi:10.1639/0007-2745(2005)108[27:eoplog]2.0.co;2

Cited by 29 publications

(36 citation statements)

References 18 publications

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“…However, we also observed 5108 polyploid plants without any growth deficiencies (7.50 %, Table 1), thus there is no close correlation between these two features. This is in agreement with a previous report which evaluated the growth of diploid and tetraploid untransformed Physcomitrella plants and found a correlation coefficient of 0.55 between growth rate on Knop medium and ploidy level (Schween et al, 2005). 1814 (= 2.67 %) haploid plants grew more slowly on Knop medium or died during two subcultures, but had a normal colony diameter on supplemented medium (Figs.…”

Section: Auxotrophic Transformantssupporting

confidence: 93%

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Large‐Scale Analysis of 73 329 Physcomitrella Plants Transformed with Different Gene Disruption Libraries: Production Parameters and Mutant Phenotypes

et al. 2005

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Gene targeting in the moss Physcomitrella patens has created a new platform for plant functional genomics. We produced a mutant collection of 73 329 Physcomitrella plants and evaluated the phenotype of each transformant in comparison to wild type Physcomitrella. Production parameters and morphological changes in 16 categories, such as plant structure, colour, coverage with gametophores, cell shape, etc., were listed and all data were compiled in a database (mossDB). Our mutant collection consists of at least 1804 auxotrophic mutants which showed growth defects on minimal Knop medium but were rescued on supplemented medium. 8129 haploid and 11 068 polyploid transformants had morphological alterations. 9 % of the haploid transformants had deviations in the leaf shape, 7 % developed less gametophores or had a different leaf cell shape. Other morphological deviations in plant structure, colour, and uniformity of leaves on a moss colony were less frequently observed. Preculture conditions of the plant material and the cDNA library (representing genes from either protonema, gametophore or sporophyte tissue) used to transform Physcomitrella had an effect on the number of transformants per transformation. We found correlations between ploidy level and plant morphology and growth rate on Knop medium. In haploid transformants correlations between the percentage of plants with specific phenotypes and the cDNA library used for transformation were detected. The number of different cDNAs present during transformation had no effect on the number of transformants per transformation, but it had an effect on the overall percentage of plants with phenotypic deviations. We conclude that by linking incoming molecular, proteome, and metabolome data of the transformants in the future, the database mossDB will be a valuable biological resource for systems biology.

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Section: Auxotrophic Transformantssupporting

confidence: 93%

“…This is in agreement with previous results which examined about 500 non-transformed plants. A multitude of different phenotypic changes were observed in polyploid plants, but changes in leaf shape and multiple phenotypic deviations from the wild type were the most likely indications of polyploidization (Schween et al, 2005).…”

Section: Morphological Phenotypesmentioning

confidence: 99%

Large‐Scale Analysis of 73 329 Physcomitrella Plants Transformed with Different Gene Disruption Libraries: Production Parameters and Mutant Phenotypes

et al. 2005

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“…The moss P. patens belongs to the Funariaceae , is haploid, monoecious and self-fertile. Polyploidization occurs rather frequently during transfection of P. patens protoplasts [36]. Among transformants, diploid plants cannot be distinguished from haploid plants using morphological traits alone [37].…”

Section: Resultsmentioning

confidence: 99%

An ancient genome duplication contributed to the abundance of metabolic genes in the moss Physcomitrella patens

et al. 2007

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“…Aberrant leaf shapes of x-ray-treated plants (Harrison et al, 2009) resemble the aberrant leaf shapes of DPpRecQ4 and are consistent with deletion of stem cells. As all DPpRecQ4 lines described here are haploid, we exclude morphological deviations like changes in leaf shape described for diploid lines (Schween et al, 2005).…”

Section: Recq Functions In Moss Developmentmentioning

confidence: 99%

RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens

et al. 2018

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RecQ DNA helicases are genome surveillance proteins found in all kingdoms of life. They are characterized best in humans, as mutations in genes lead to developmental abnormalities and diseases. To better understand RecQ functions in plants we concentrated on and , the model species predominantly used for studies on DNA repair and gene targeting. Phylogenetic analysis of the six genes revealed their orthologs in humans and plants. Because Arabidopsis and differ in their and genes, reporter and deletion moss mutants were generated and gene functions studied in reciprocal cross-species and cross-kingdom approaches. Both proteins can be found in meristematic moss tissues, although at low levels and with distinct expression patterns.RecQ4 is involved in embryogenesis and in subsequent development as demonstrated by sterility of Δ mutants and by morphological aberrations. Additionally, Δ displays an increased sensitivity to DNA damages and an increased rate of gene targeting. Therefore, we conclude that RecQ4 acts as a repressor of recombination. In contrast,RecQ6 is not obviously important for moss development or DNA repair but does function as a potent enhancer of gene targeting.

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Effect of Ploidy Level on Growth, Differentiation, and Morphology in Physcomitrella patens

Cited by 29 publications

References 18 publications

Large‐Scale Analysis of 73 329 Physcomitrella Plants Transformed with Different Gene Disruption Libraries: Production Parameters and Mutant Phenotypes

Large‐Scale Analysis of 73 329 Physcomitrella Plants Transformed with Different Gene Disruption Libraries: Production Parameters and Mutant Phenotypes

An ancient genome duplication contributed to the abundance of metabolic genes in the moss Physcomitrella patens

RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens

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