Effect of platelet-rich plasma (PRP) on post-thaw quality, kinematics and in vivo fertility of fertile and subfertile buffalo (Bubalus bubalis) spermatozoa

Almadaly, Essam A.; Ibrahim, Ibrahim M.; Salama, M.; Ashour, Mohey A; Sahwan, Ferial M.; El-Kon, Ismail; Abouzed, Tarek K.; Eldomany, Wael

doi:10.1007/s11259-022-09928-1

Cited by 2 publications

(5 citation statements)

References 48 publications

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“…This is consistent with the findings of Kumar et al [33] who found that the postthaw membrane integrity, viability, and motility of buffalo bull spermatozoa were enhanced by adding 250 ng/mL IGF-1 to buffalo semen before cryopreservation. Also, these findings emphasized our recent report that revealed supplementing of 15 % PRP to the semen extender was successful in increasing post-thaw antioxidant activity, quality, and in vivo fertility of buffalo bull spermatozoa [5].…”

Section: Discussionsupporting

confidence: 78%

“…Herein and in our recent report [5], IGF-1 concentrations in PRP extracted from the blood of fertile buffalo bulls varied from 1350 to 2150 ng/ mL, with an average of 1750 ng/mL. As a result, in the current study, 15 % PRP (≈250 ng/mL IGF-1) supplementation improved post-thaw sperm quality, increasing in vitro fertility of buffalo bull spermatozoa.…”

Section: Discussionsupporting

confidence: 55%

“…Cryodamage of sperm is provoked primarily by cold shock, osmotic alterations, the formation of intracellular ice crystals, and oxidative stress [2]. Considering this, several cryoprotectants, such as fatty acids, antioxidants, peptide compounds, nanomaterial particles, animal serum, plant essential oils, and bioactive analogues, have been suggested and developed [3], also platelet-rich plasma (PRP), was incorporated into semen extender to improve frozen-thawed human [4] and bovine [5] sperm quality.…”

Section: Introductionmentioning

confidence: 99%

“…Platelet-dense granules are components of PRP, which contain histamine, adenosine triphosphate, zinc and calcium ions, superoxide dismutase (SOD), and serotonin, which are crucial for regulating cell and tissue homeostasis [8]. However, PRP components have improved sperm quality, function, and antioxidant activity in human [4,9] and bovine [5] semen and a few publications are available on its effect on buffalo semen. Therefore, the current study investigates the possible impacts of incorporating various concentrations of autologous PRP into the extender on post-thaw semen properties, antioxidant enzymatic activity, and in vitro fertility of buffalo bull spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Adding Autologous Platelet-rich Plasma to the Freezing Extender on the Post-thaw Quality and In vitro Fertility of Buffalo (Bubalus Bubalis) Spermatozoa

Almadaly,

Mansour,

Sahwan

et al. 2024

Egyptian Journal of Veterinary Sciences

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T HIS STUDY investigates the consequence of adding platelet-rich plasma (PRP) on the post-thawed quality and in vitro fertility of buffalo bull semen. Fiftyfour ejaculates from nine buffalo bulls were collected, and every ejaculate was then dispensed into 5 equal parts, diluted with Optixcell extender supplemented with five [0, 5, 10, 15, and 20] proportions of PRP followed by freezing using the usual protocol. Post-thaw sperm motility, sperm viability, the integrity (%) of the plasma membrane, acrosome, DNA, and mitochondrial membrane potential (%) were evaluated. Total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) content as well as in vitro fertilization (IVF) were also assessed. The obtained results revealed that the 15 % PRP-supplemented extender improved sperm viability, progressive motility, and integrity of the plasma membrane. All 5 %, 10 %, and 15 % PRP treatments greatly reduced (P < 0.05) MDA and increased GPx and SOD activities. The cleavage rate was greatly increased (P < 0.05) following IVF with 15 % PRP-supplemented spermatozoa. In conclusion, the incorporation of 15 % PRP into the freezing extender is recommended to ameliorate freezing-thawing damage on sperm cell membranes and increase the in vitro fertility of buffalo bull spermatozoa.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Adding Autologous Platelet-rich Plasma to the Freezing Extender on the Post-thaw Quality and In vitro Fertility of Buffalo (Bubalus Bubalis) Spermatozoa

Almadaly,

Mansour,

Sahwan

et al. 2024

Egyptian Journal of Veterinary Sciences

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show abstract

“…PRP has been found to have anti-apoptotic and antioxidant properties in mammalian cells 86 . Before cryopreservation of semen; PRP is added to the semen extender to increase the antioxidant status and reduce the oxidative stress [87][88][89][90] . PRP was reported to improve ovarian TAC levels and diminish MDA levels in PCOS-induced pathogenesis 91 .…”

Section: Microbiological Evaluationmentioning

confidence: 99%

Platelet-rich plasma attenuates the UPEC-induced cystitis via inhibiting MMP-2,9 activities and downregulation of NGF and VEGF in Canis Lupus Familiaris model

Abdelgalil,

Yassin,

Khattab

et al. 2024

Sci Rep

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One of the most prevalent disorders of the urinary system is urinary tract infection, which is mostly brought on by uropathogenic Escherichia coli (UPEC). The objective of this study was to evaluate the regenerative therapeutic and antibacterial efficacy of PRP for induced bacterial cystitis in dogs in comparison to conventional antibiotics. 25 healthy male mongrel dogs were divided into 5 groups (n = 5). Control negative group that received neither induced infection nor treatments. 20 dogs were randomized into 4 groups after two weeks of induction of UPEC cystitis into; Group 1 (control positive; G1) received weekly intravesicular instillation of sodium chloride 0.9%. Group 2 (syst/PRP; G2), treated with both systemic intramuscular antibiotic and weekly intravesicular instillation of PRP; Group 3 (PRP; G3), treated with weekly intravesicular instillation of PRP, and Group 4 (syst; G4) treated with an intramuscular systemic antibiotic. Animals were subjected to weekly clinical, ultrasonographic evaluation, urinary microbiological analysis, and redox status biomarkers estimation. Urinary matrix metalloproteinases (MMP-2, MMP-9) and urinary gene expression for platelet-derived growth factor -B (PDGF-B), nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) were measured. At the end of the study, dogs were euthanized, and the bladder tissues were examined macroscopically, histologically, and immunohistochemically for NF-κB P65 and Cox-2. The PRP-treated group showed significant improvement for all the clinical, Doppler parameters, and the urinary redox status (p < 0.05). The urinary MMPs activity was significantly decreased in the PRP-treated group and the expression level of urinary NGF and VEGF were downregulated while PDGFB was significantly upregulated (p < 0.05). Meanwhile, the urinary viable cell count was significantly reduced in all treatments (P < 0.05). Gross examination of bladder tissue showed marked improvement for the PRP-treated group, expressed in the histopathological findings. Immunohistochemical analysis revealed a marked increase in Cox-2 and NF-κB P65 in the PRP-treated group (P < 0.05). autologous CaCl2-activated PRP was able to overcome the bacterial infection, generating an inflammatory environment to overcome the old one and initiate tissue healing. Hence, PRP is a promising alternative therapeutic for UPEC cystitis instead of conventional antibiotics.

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Effect of platelet-rich plasma (PRP) on post-thaw quality, kinematics and in vivo fertility of fertile and subfertile buffalo (Bubalus bubalis) spermatozoa

Cited by 2 publications

References 48 publications

Effect of Adding Autologous Platelet-rich Plasma to the Freezing Extender on the Post-thaw Quality and In vitro Fertility of Buffalo (Bubalus Bubalis) Spermatozoa

Effect of Adding Autologous Platelet-rich Plasma to the Freezing Extender on the Post-thaw Quality and In vitro Fertility of Buffalo (Bubalus Bubalis) Spermatozoa

Platelet-rich plasma attenuates the UPEC-induced cystitis via inhibiting MMP-2,9 activities and downregulation of NGF and VEGF in Canis Lupus Familiaris model

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