Effect of Platelet‐Rich Plasma on Cell Adhesion, Cell Migration, and Myofibroblastic Differentiation in Human Gingival Fibroblasts

Abstract

Platelet-rich plasma may modulate several cell responses potentially involved in wound healing such as cell adhesion, cell migration, and myofibroblastic differentiation.

Placental Perivascular Cells for Human Muscle Regeneration

“…Adhesion regulates multiple aspects of cell life via a variety of molecules and ligands [30,[34][35][36][37]. To test the behavior of villus-derived cells in the presence of different adhesion molecules, sorted CD146þ and CD146À cells were seeded on different extracellular matrix substrates.…”

Development and bioevaluation of nanofibers with blood-derived growth factors for dermal wound healing

“…The effects of nanofibers with incorporated 38 platelet-rich plasma on cell proliferation, survival, morphology and mobility were examined. Nanofibers [1][2][3]. Tissue injury is followed by several responses of 54 cells associated with wounded site, such as cell adhesion, prolifer-55 ation, differentiation and migration, synthesis and remodeling of 56 extracellular matrix [1,2,4].…”

“…Nanofibers [1][2][3]. Tissue injury is followed by several responses of 54 cells associated with wounded site, such as cell adhesion, prolifer-55 ation, differentiation and migration, synthesis and remodeling of 56 extracellular matrix [1,2,4]. The repair process, initiated immedi-57 ately after injury, is moderated by the interplay of various growth 58 factors, cytokines and low molecular weight compounds released 59 during platelet degranulation [3].…”

“…60 Growth factors are polypeptides that act as tools for intercellu-61 lar communication and control of cell fate. They exert their effects 62 locally as regulators of basic cell functions [1,2,5]. Platelets are a 63 well-known natural source of growth factors and are prepared 64 from anticoagulated blood by a separation procedure, which 65 involves a two stage centrifugation.…”

Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton

“…15 Several other types of cells, beside osteoblasts, have been shown to acquire migration capacity and in some cases to undergo cytoskeletal changes when primed with PDGF: among them hepatic stellate cells, 23 marrow-derived mesenchymal stem cells, 24 malignant mesothelioma cells, 25 subchondral progenitors, 26 gingival fibroblasts. 27 The clinical use of PRP has recently experienced a surge in different indications and in particular in the procedures involving healing and tissue regeneration, as in dental and oral surgery, 9 in bone deficiency healing, 3,7,10 in the treatment for tendon and ligament injuries, 8,11 in the pathologic processes of articular cartilage. 12 In all these indications the recruitment of cells in the site of the lesion might represent the initial and crucial step in the process of healing.…”