Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis

Abstract

Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for complication of diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in diabetic rat testis. The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements. Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.

Protective Effects of Autologous Platelet-Rich Plasma (PRP) on the Outcome of Cryopreservation in Rabbit Sperm

“…Based on those results, we suggest the intratesticular injection of PRP may have an important molecular role to enhance the testicular cells to the regulation of CATSPER, SPAG5 and Hsp70, this result was confirmed by the researcher (40) were suggested the PRP contains the highest concentration of growth factors with known anabolic enhances tendon matrix gene expression patterns. However, there was no difference in CASPASE-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to those without PRP (41). Furthermore, the freeze-thaw process of cryopreservation can modulate mRNA stability, protein and gene expression, and epigenetic content of sperm (42).…”