Effect of platelet-rich plasma concentrations on the proliferation of periodontal cells: An in vitro study

Abstract

Objective:The purpose of this study was to evaluate the effect of different concentrations of platelet-rich plasma (PRP) on the proliferation of undifferentiated periodontal ligament (PDL) fibroblasts.Materials and Methods:The undifferentiated PDL fibroblasts were obtained from two healthy human premolar teeth and cultured in Dulbecco's modified Eagle's medium. Cell wells were divided into five groups. Experimental groups received 0.1%, 5%, or 50% PRP; the positive and negative control groups were cultured in fetal bovine serum (FBS) 12% and in a medium without FBS 12%, respectively. The plates were incubated at 37°C for 1, 2, 3, 4, and 7 days. PDL cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide proliferation assay. Statistical analysis of the data was accomplished using repeated measure ANOVA and Tukey's test. P < 0.05 was considered statistically significant.Results:The 5% PRP had the greatest effect on undifferentiated fibroblast proliferation, which was significant on the 3rd day. There was no significant difference between 0.1% PRP and positive control during the first 3 days. The group with 50% PRP presented significantly lower proliferation, compared to other experimental and control groups.Conclusions:It may be concluded that the growth-stimulating effect of PRP is dose dependent with the best results in low concentrations.

Comparison of Connective Tissue Graft and Platelet Rich Fibrin as Matrices in A Novel Papillary Augmentation Access: A Randomized Controlled Clinical Trial

“…In the present study, a novel papillary augmentation access was proposed where both buccal and palatal tunneling was performed with the placement of CTG and PRF [22][23][24][25] as matrices, and the papilla being displaced as a gingivopapillary unit.…”

Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells

“…The addition of 10% PRP to the culture medium stimulated more mitogenic response in PDL cells than the addition of 5% PRP; however, this difference was not statistically significant. From a previous study,[ 7 ] 5% PRP had a greater stimulating impact on cellular proliferation but 50% PRP adversely affected cell vitality. Creeper et al .…”

“…A recent study showed that PRP could stimulate PDL fibroblast proliferation. [ 7 ] However, the regenerative events also require migration and attachment of connective tissue cells to the wound site and synthesis of the specialized components which they are attempting to repair. Thus, the aim of this study was to evaluate the effects of PRP on the proliferation, migration, and attachment of PDL cells in vitro .…”

Untitled

“…The addition of 10% PRP to the culture medium stimulated more mitogenic response in PDL cells than the addition of 5% PRP; however, this difference was not statistically significant. From a previous study, [7] 5% PRP had a greater stimulating impact on cellular proliferation but 50% PRP adversely affected cell vitality. Creeper et al [20] reported a cytotoxic effect of 100% PRP.…”