Effect of platelet-rich plasma and platelet-rich fibrin matrix on healing of vertical meniscal tears in a rabbit model

Kurnaz, Recep; Balta, Orhan

doi:10.5152/j.aott.2020.02.20

Cited by 13 publications

(11 citation statements)

References 40 publications

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“…This model thus may provide a well-defined test bed for the assessment of novel repair approaches for these types of tears. Indeed, many groups have used a vertical longitudinal tear during ex vivo and large-animal assessment of biologic, 38 , 74 , 78 , 83 scaffold-based, 5 , 62 , 65 or surgical repair 2 , 4 , 12 , 16 , 48 , 68 strategies. The stability of this defect and lack of healing observed in the meniscus itself indicate its utility for such studies.…”

Section: Discussionmentioning

confidence: 99%

Six-Month Outcomes of Clinically Relevant Meniscal Injury in a Large-Animal Model

Bansal

Meadows

Miller

et al. 2021

Orthopaedic Journal of Sports Medicine

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Background: The corrective procedures for meniscal injury are dependent on tear type, severity, and location. Vertical longitudinal tears are common in young and active individuals, but their natural progression and impact on osteoarthritis (OA) development are not known. Root tears are challenging and they often indicate poor outcomes, although the timing and mechanisms of initiation of joint dysfunction are poorly understood, particularly in large-animal and human models. Purpose/Hypothesis: In this study, vertical longitudinal and root tears were made in a large-animal model to determine the progression of joint-wide dysfunction. We hypothesized that OA onset and progression would depend on the extent of injury-based load disruption in the tissue, such that root tears would cause earlier and more severe changes to the joint. Study Design: Controlled laboratory study. Methods: Sham surgeries and procedures to create either vertical longitudinal or root tears were performed in juvenile Yucatan mini pigs through randomized and bilateral arthroscopic procedures. Animals were sacrificed at 1, 3, or 6 months after injury and assessed at the joint and tissue level for evidence of OA. Functional measures of joint load transfer, cartilage indentation mechanics, and meniscal tensile properties were performed, as well as histological evaluation of the cartilage, meniscus, and synovium. Results: Outcomes suggested a progressive and sustained degeneration of the knee joint and meniscus after root tear, as evidenced by histological analysis of the cartilage and meniscus. This occurred in spite of spontaneous reattachment of the root, suggesting that this reattachment did not fully restore the function of the native attachment. In contrast, the vertical longitudinal tear did not cause significant changes to the joint, with only mild differences compared with sham surgery at the 6-month time point. Conclusion: Given that the root tear, which severs circumferential connectivity and load transfer, caused more intense OA compared with the circumferentially stable vertical longitudinal tear, our findings suggest that without timely and mechanically competent fixation, root tears may cause irreversible joint damage. Clinical Relevance: More generally, this new model can serve as a test bed for experimental surgical, scaffold-based, and small molecule–driven interventions after injury to prevent OA progression.

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Section: Discussionmentioning

confidence: 99%

Six-Month Outcomes of Clinically Relevant Meniscal Injury in a Large-Animal Model

Bansal

Meadows

Miller

et al. 2021

Orthopaedic Journal of Sports Medicine

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“…PRP has shown the capacity to inhibit inflammation and improve cartilage tissue regeneration by promoting the synthesis of cartilage matrix, increasing cell growth and migration, and inducing phenotype changes [133,153]. In the field of meniscus tissue engineering (MTE), PRP has been incorporated into various hydrogel systems and has demonstrated high potential for meniscus repair [132,133,[155][156][157][158][159]. It is worth mentioning that PRP contains fibrinogen, which can be activated by thrombin and Ca 2+ to form a hydrogel.…”

Section: Platelet-rich Plasma (Prp)mentioning

confidence: 99%

Advances in Hydrogels for Meniscus Tissue Engineering: A Focus on Biomaterials, Crosslinking, Therapeutic Additives

Zhou,

Wang,

Jiang

et al. 2024

Gels

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Meniscus tissue engineering has emerged as a promising strategy for meniscus repair and regeneration. As versatile platforms, hydrogels have gained significant attention in this field, as they possess tunable properties that allow them to mimic native extracellular matrices and provide a suitable microenvironment. Additionally, hydrogels can be minimally invasively injected and can be adjusted to match the shape of the implant site. They can conveniently and effectively deliver bioactive additives and demonstrate good compatibility with other functional materials. These inherent qualities have made hydrogel a promising candidate for therapeutic approaches in meniscus repair and regeneration. This article provides a comprehensive review of the advancements made in the research on hydrogel application for meniscus tissue engineering. Firstly, the biomaterials and crosslinking strategies used in the formation of hydrogels are summarized and analyzed. Subsequently, the role of therapeutic additives, including cells, growth factors, and other active products, in facilitating meniscus repair and regeneration is thoroughly discussed. Furthermore, we summarize the key issues for designing hydrogels used in MTE. Finally, we conclude with the current challenges encountered by hydrogel applications and suggest potential solutions for addressing these challenges in the field of MTE. We hope this review provides a resource for researchers and practitioners interested in this field, thereby facilitating the exploration of new design possibilities.

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“…PRF enhanced the synthesis and deposition of ECM by cultured meniscocytes, which were evaluated both morphologically and histologically [87]. Kurnaz et al concluded that PRP and PRF matrix augmentation on vertical meniscal tears in a rabbit model resulted in early recovery and enhanced repair of meniscus tissue [88]. The role of PRF in terms of healing and regeneration of meniscus tissue needs to be explored.…”

Section: Meniscal Injuriesmentioning

confidence: 99%

Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration

Narayanaswamy¹,

Patro

Jeyaraman³

et al. 2023

Bioengineering

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Over the past few decades, various forms of platelet concentrates have evolved with significant clinical utility. The newer generation products, including leukocyte-platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF), have shown superior biological properties in musculoskeletal regeneration than the first-generation concentrates, such as platelet-rich plasma (PRP) and plasma rich in growth factors. These newer platelet concentrates have a complete matrix of physiological fibrin that acts as a scaffold with a three-dimensional (3D) architecture. Further, it facilitates intercellular signaling and migration, thereby promoting angiogenic, chondrogenic, and osteogenic activities. A-PRF with higher leukocyte inclusion possesses antimicrobial activity than the first generations. Due to the presence of enormous amounts of growth factors and anti-inflammatory cytokines that are released, A-PRF has the potential to replicate the various physiological and immunological factors of wound healing. In addition, there are more neutrophils, monocytes, and macrophages, all of which secrete essential chemotactic molecules. As a result, both L-PRF and A-PRF are used in the management of musculoskeletal conditions, such as chondral injuries, tendinopathies, tissue regeneration, and other sports-related injuries. In addition to this, its applications have been expanded to include the fields of reconstructive cosmetic surgery, wound healing in diabetic patients, and maxillofacial surgeries.

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Effect of platelet-rich plasma and platelet-rich fibrin matrix on healing of vertical meniscal tears in a rabbit model

Abstract: The role of the platelet-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in the prediction of length and cost of hospital stay in patients with infected diabetic foot ulcers: A retrospective comparative study

Cited by 13 publications

References 40 publications

Six-Month Outcomes of Clinically Relevant Meniscal Injury in a Large-Animal Model

Six-Month Outcomes of Clinically Relevant Meniscal Injury in a Large-Animal Model

Advances in Hydrogels for Meniscus Tissue Engineering: A Focus on Biomaterials, Crosslinking, Therapeutic Additives

Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration

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