Effect of platelet count on platelet aggregation measured with impedance aggregometry (Multiplate™ analyzer) and with light transmission aggregometry

Femia, Eti Alessandra; Scavone, Mariangela; Lecchi, Anna; Cattaneo, Marco

doi:10.1111/jth.12432

Cited by 75 publications

(66 citation statements)

References 11 publications

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“…Previous studies of healthy volunteers and patients with stable CAD have found that TPC affects Multiplate results, especially when platelet counts are low, but no studies have been identified that tested the blood of patients with actual thrombocytopenia [1][2][3][4][15][16][17]. We found a significant association between TPC and AUC for all four agonists in patients with severe thrombocytopenia, which is in concordance with previous studies in healthy volunteers and CAD patients.…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

Multiple electrode aggregometry in thrombocytopenic haemato-oncologic patients – Influence of haematologic variables

Opheim¹,

Apelseth²,

Stanworth³

et al. 2017

Hematol Med Oncol

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Section: Discussionsupporting

confidence: 82%

“…Studies on healthy volunteers and patients with stable coronary artery disease (CAD) have demonstrated that platelet counts affect the Multiplate analysis [1][2][3]. Studies involving healthy volunteers and stable CAD patients on low dose aspirin treatment also report the impact of white blood cell counts (WBC) on Multiplate results [2,4].…”

Section: Introductionmentioning

confidence: 99%

Multiple electrode aggregometry in thrombocytopenic haemato-oncologic patients – Influence of haematologic variables

Opheim¹,

Apelseth²,

Stanworth³

et al. 2017

Hematol Med Oncol

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“…In contrast, with lumi‐LTA this was only observed with collagen but not ADP, PAR‐1 or arachidonic acid (see supplementary figures). Previous studies with lumi‐LTA have found no correlation between platelet count and aggregation results . Seyfert et al .…”

Section: Discussionmentioning

confidence: 86%

Comparison of multiple electrode aggregometry with lumi‐aggregometry for the diagnosis of patients with mild bleeding disorders

Ghaithi

Drake

Watson

et al. 2017

Journal of Thrombosis and Haemostasis

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Background Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing preanalytical errors associated with preparation of platelet-rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents (e.g. aspirin and clopidogrel). Objective Our current study sought to evaluate the ability of MEA in diagnosing patients with mild bleeding disorders by comparison with light transmission lumi-aggregometry (lumi-LTA). Methods Forty healthy subjects and 109 patients with a clinical diagnosis of a mild bleeding disorder were recruited into the UK Genotyping and Phenotyping of Platelets study (GAPP, ISRCTN 77951167). MEA was performed on whole blood using one or two concentrations of ADP, PAR-1 peptide, arachidonic acid and collagen. Lumi-LTA was performed in PRP using several concentrations of ADP, adrenaline, arachidonic acid, collagen, PAR-1 peptide and ristocetin. Results Of 109 patients tested, 54 (49%) patients gave abnormal responses by lumi-LTA to one or more agonists. In contrast, only 16 (15%) patients were shown to have abnormal responses to one or more agonists by MEA. Conclusions In this study we showed that MEA is less sensitive in identifying patients with abnormal platelet function relative to lumi-LTA.

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“…The results were expressed as maximal light transmission. The LTA of the PFSC (750,000 platelets/μl, n = 10), PSSC (1,500,000 platelets/μl, n = 10) and PLSSP (2,000,000 platelets/μl, n = 10) were statistically compared 35.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, development, characterization and effectiveness of bovine pure platelet gel‐collagen‐polydioxanone bioactive graft on tendon healing

Moshiri

Oryan

Meimandi-Parizi

2015

J Cellular Molecular Medi

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Bovine platelet gel (BPG) is an accessible and cost-effective source of growth factors which may have a value in tendon regenerative medicine. We produced a collagen implant (CI) as a tendon proper, covered it with polydioxanone (PDS) sheath to simulate paratenon and finally embedded the BPG as an active source of growth factor within the bioimplant to test whether BPG would be able to accelerate and enhance tendon regeneration and repair. After in vitro characterization of the bioactive grafts, the grafts were implanted in rabbit large tendon defect model. Untreated tendons and tendons treated with either CI or CI-PDS were served as controls for the CI-PDS-BPG. The animals were investigated clinically, ultrasonographically and haematologically for 120 days. After euthanasia, dry matter content, water uptake and delivery characteristics and also gross morphological, histopathological and scanning electron microscopic features of the healing tendons were assessed. In vitro, the activated platelets in the scaffold, released their growth factors significantly more than the controls. BPG also increased cell viability, and enhanced cellular differentiation, maturation and proliferation inside the CI-PDS compared with the controls. In vivo, the BPG modulated inflammation, increased quality and rate of fibroplasia and produced a remodelled tendon that had significantly higher collagen content and superior collagen fibril and fibre differentiation than controls. Treatment also significantly improved tendon water uptake and delivery characteristics, animals’ serum PDGF level, CI-PDS biocompatibility and biodegradability and reduced peritendinous adhesions, muscle fibrosis and atrophy. BPG was effective on tendon healing and CI-PDS-BPG may be a valuable bioscaffold in tendon reconstructive surgery.

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Effect of platelet count on platelet aggregation measured with impedance aggregometry (Multiplate™ analyzer) and with light transmission aggregometry

Cited by 75 publications

References 11 publications

Multiple electrode aggregometry in thrombocytopenic haemato-oncologic patients – Influence of haematologic variables

Multiple electrode aggregometry in thrombocytopenic haemato-oncologic patients – Influence of haematologic variables

Comparison of multiple electrode aggregometry with lumi‐aggregometry for the diagnosis of patients with mild bleeding disorders

Synthesis, development, characterization and effectiveness of bovine pure platelet gel‐collagen‐polydioxanone bioactive graft on tendon healing

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