Effect of plasma processing and storage on microparticle abundance, nitric oxide scavenging, and vasoactivity

Rogers, Stephen C.; Moynihan, Francis T.; McDonough, Ryan; Timm, David D.; Hovmand‐Warner, Eyvind; Frazier, Elfaridah P.; Thomas, Kimberly A.; Spinella, Philip C.; Doctor, Allan

doi:10.1111/trf.15214

Cited by 8 publications

(7 citation statements)

References 20 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Standardization of EV analyses of whole blood samples and definitions of normal ranges are warranted. EV assessment could get part of the quality assessment of new extracorporeal devices including cell savers [31,32]. Our aim was, on the one hand, to document the cellular origin of the vesicles as much as possible and, on the other hand, to use the simplest and fastest routinely applicable method that could represent a further step towards standardization.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles in Autologous Cell Salvaged Blood in Orthopedic Surgery

et al. 2021

View full text Add to dashboard Cite

(1) Background: Cell salvage is highly recommended in orthopedic surgery to avoid allogeneic transfusions. Preparational steps during cell salvage may induce extracellular vesicle (EV) formation with potential thrombogenic activity. The purpose of our study was to assess the appearance of EVs at retransfusion. (2) Methods: After ethics committee approval and informed consent, blood was withdrawn from the autotransfusion system (Xtra, Sorin, Germany) of 23 patients undergoing joint arthroplasty. EVs were assessed by flow cytometry in two times centrifugated samples. EVs were stained with specific antibodies against cellular origins from platelets (CD41), myeloid cells (CD15), monocytes (CD14), and erythrocytes (CD235a). The measured events/µL in the flow cytometer were corrected to the number of EVs in the retransfusate. (3) Results: We measured low event rates of EVs from platelets and myeloid origin (<1 event/µL) and from monocytic origin (<2 events/µL). Mean event rates of 17,042 events/µL (range 12–81,164 events/µL) were found for EVs from red blood cells. (4) Conclusion: Retransfusate contains negligible amounts of potentially thrombogenic EVs from platelet and monocytic origin. Frequent EVs from erythrocytes may indicate red blood cell destruction and/or activation during autologous cell salvage. Further research is needed to investigate the clinical relevance of EVs from salvaged red blood cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles in Autologous Cell Salvaged Blood in Orthopedic Surgery

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The NO consumption assay was performed as previously reported 63,64 . A custom□made glass reaction cell was filled with PBS (4 mL, pH 7.4, 37°C) and connected with a flow meter and a TEA NO analyzer.…”

Section: Methodsmentioning

confidence: 99%

Horizontal gene transfer in the human and skin commensalMalassezia: a bacterially-derived flavohemoglobin is required for NO resistance and host interaction

Ianiri

Ruchti

et al. 2020

Preprint

View full text Add to dashboard Cite

The skin of humans and animals is colonized by commensal and pathogenic fungi and bacteria that share this ecological niche and have established microbial interactions. Malassezia are the most abundant fungal skin inhabitant of warm-blooded animals, and have been implicated in skin diseases and systemic disorders, including Crohn’s disease and pancreatic cancer. Flavohemoglobin is a key enzyme involved in microbial nitrosative stress resistance and nitric oxide degradation. Comparative genomics and phylogenetic analyses within the Malassezia genus revealed that flavohemoglobin-encoding genes were acquired through independent horizontal gene transfer events from different donor bacteria that are part of the mammalian microbiome. Through targeted gene deletion and functional complementation in M. sympodialis, we demonstrated that bacterially-derived flavohemoglobins are cytoplasmic proteins required for nitric oxide detoxification and nitrosative stress resistance under aerobic conditions. RNAseq analysis revealed that endogenous accumulation of nitric oxide resulted in upregulation of genes involved in stress response, and downregulation of the MalaS7 allergen-encoding genes. Solution of the high-resolution X-ray crystal structure of Malassezia flavohemoglobin revealed features conserved with both bacterial and fungal flavohemoglobins. In vivo pathogenesis is independent of Malassezia flavohemoglobin. Lastly, we identified additional 30 genus- and species-specific horizontal gene transfer candidates that might have contributed to the evolution of this genus as the most common inhabitants of animal skin.Significance statementMalassezia species are the main fungal components of the mammalian skin microbiome and are associated with a number of skin disorders. Recently, Malassezia has also been found in association with Crohn’s Disease and with pancreatic cancer. The elucidation of the molecular bases of skin adaptation by Malassezia is critical to understand its role as commensal and pathogen. In this study we employed evolutionary, molecular, biochemical, and structural analyses to demonstrate that the bacterially-derived flavohemoglobins acquired by Malassezia through horizontal gene transfer resulted in a gain of function critical for nitric oxide detoxification and resistance to nitrosative stress. Our study underscores horizontal gene transfer as an important force modulating Malassezia evolution and niche adaptation.

show abstract

“…The washing process eliminates lipid‐enveloped viruses, cells, and cell‐derived debris from the product, including cytokines 87 . In addition, the amount of residual cells and cell‐derived micro particles is lower in SDP when compared to FFP 88,89 . Reduction of all these mediators may theoretically reduce immunologic effects of plasma transfusion.…”

Section: Differences Between Plasma Productsmentioning

confidence: 99%