This study was conducted at Vegetable Research Farm, Department of Horticulture, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad (U.P.) during the rabi season of 2016 to 2017 to find out the optimum time of sowing and planting spacing for broccoli varieties as Palam Samridhi, Pusa Kanchan and Lufa F1 Hybrid. Seedlings were raised by sowing on three different dates viz. 20th October, 04 November and 19 November were transplanted at spacing viz. 45 cm x 45 cm. Economic return and biochemical traits of broccoli were significantly influenced by the treatments. In view of experimental results obtained during the present investigation, treatment T4 D2 V1 04 November + V1 Palam Samridhi emerged as superior over all other treatments, in relation to economic return, cost benefit ratio and biochemical traits of broccoli under the agro-climatic condition of Allahabad.