Effect of Plant Growth Regulator Combination on Direct In Vitro Regeneration of Persian Lilac (Melia azedarach L.)

Amiri, Saleh; Panahi, Bahman; Mohammadi, Reza; Fattahi, Fatemeh

doi:10.1007/s40011-019-01099-5

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…Direct multiple shoot induction is the appropriate method for plantlet propagation from mature trees with a lower risk of genetic variability compared to other regeneration methods, and it is a more reliable for clonal propagation [12][13][14][15] .…”

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confidence: 99%

Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

Amiri

Mohammadi

2021

Sci Rep

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The conventional reproduction methods are not efficient for regeneration of Sumac (Rhus coriaria L.). The purpose of this work was to study the micropropagation of R. coriaria using lateral buds as explant in Murashige and Skoog (MS) medium with different concentrations of plant growth regulator (PGRs). Four concentrations of Benzylaminopurine (BAP) in combination with three concentrations of indol-3-butyric acid (IBA) and 1.0 mg/L gibberellic acid (GA3) were tested for establishment and shoot multiplication. For root induction, IBA was used at four levels combined with 0, 0.5 and 1 mg/L of naphthalene acetic acid (NAA) in full and half strength of MS medium. BAP at 2 mg/L with 1 mg/L IBA was best, with 88.88% of establishment. The highest shoot proliferation (12.30 ± 0.30) was obtained in medium fortified with 2 mg/L BAP plus 0.5 mg/L IBA and the highest shoot length (8.50 cm) was obtained at 3 mg/L BAP plus 1 mg/L IBA. The highest rooting (100%) was observed in 1/2-strength MS medium containing 1 mg/L IBA with 0.5 mg/L NAA. In conclusion, an efficient protocol with high rate of proliferation and rooting is described for R. coriaria, which can be used in massive propagation.

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confidence: 99%

Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

Amiri

Mohammadi

2021

Sci Rep

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“…Factors such as the use of adult material, secretion of inhibitors, and accumulation of ethylene during rooting can all negatively affect root formation [24]. Root induction and proliferation are affected not only by the type of auxin but also by the concentration and the duration of the auxin treatment, as well as by the culture medium [25]. In our experiments, rooting increased by 40-50% when IBA was used at a concentration of 14.76 µM in the culture medium.…”

Section: Discussionmentioning

confidence: 60%

Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species

Guerra

Badilla²,

Cautin

et al. 2022

Plants

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Citronella mucronata (C. mucronata), a tree species endemic to Chile, has become threatened in its natural habitat and is currently listed as vulnerable. Tree population parameters have deteriorated due to indiscriminate logging and other anthropogenic activities, warranting research on mass propagation as a means of recovery. This study, unprecedented for this native species, has developed a successful method for its micropropagation. The objective was to establish a protocol for in vitro propagation of C. mucronata to produce large quantities of high-quality seedlings in an accelerated plant acquisition process. The best results were achieved by growing explants on Murashige and Skoog (MS) basal culture medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 14.76 μM indole-3-butyric acid (IBA). Explant survival rate was 78 %, the average shoot length reached 3.2 cm, the number of lateral shoots was 3.9, and rooting rate was 60%. Furthermore, stimulation with red and blue light in a 1:2 ratio, supplemented with 14.76 μM IBA, improved the rooting rate to 93%. The survival rate of rooted explants reached 100% in the acclimatization stage when using peat and perlite substrate (1:1 v/v).

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“…So far, many other plants are symbiotic with fungi in nature, which are difficult for tissue culture and in vitro regeneration system, and B. sinensis , which is a mycorrhizal plant, is one of them. For in vitro rooting, numerous studies favor the 1/2 MS basic medium and addition of auxin to induce rooting ( Wei et al., 2017 ; Amiri et al., 2020 ). This study specifically aimed to optimize the in vitro rooting conditions for B. sinensis by comparing the efficacy of 1/2 MS and WPM media, which has lower inorganic salt concentration.…”

Section: Discussionmentioning

confidence: 99%

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

Yan,

Zheng,

et al. 2024

Front. Plant Sci.

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Bretschneidera sinensis is a monotypic species of rare and tertiary relic trees mainly distributed in China. B. sinensis is a potentially valuable horticultural plant, which has significant ornamental and research value, and is a crucial tool for the study of phylogeography. The artificial cultivation of B. sinensis is of great scientific value and practical significance. In this study, we developed a direct organogenesis process of B. sinensis using mature zygotic embryos as initial materials. The highest sterile germination induction (54.5%) from the mature zygotic embryo was obtained in a Murashige and Skoog (MS) medium with 2.0 mg·L−1 6-benzylaminopurine (6-BA) and 0.2 mg·L−1 α-naphthaleneacetic acid (NAA). The highest percentage of shoot regeneration (90.37%) was attained using 1.0 mg·L−1 6-BA and 0.01 mg·L−1 NAA in the MS medium. The Woody Plant Medium (WPM) had the greatest adventitious shoot elongation rate of 93.33%. The most optimized rooting rate was 88.89% in a half-strength MS medium containing 2.0 mg·L−1 indole-3-butyric acid (IBA) and 1.0 mg·L−1 NAA. The genetic fidelity of in vitro regenerated plantlets was assessed using inter-simple sequence repeats and random amplified polymorphic DNA molecular markers, confirming the genetic uniformity and stability of regenerated B. sinensis plantlets. Our research presents an effective in vitro propagation system for B. sinensis, laying the groundwork for its germplasm conservation and large-scale production while maintaining high genetic integrity.

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Effect of Plant Growth Regulator Combination on Direct In Vitro Regeneration of Persian Lilac (Melia azedarach L.)

Cited by 10 publications

References 18 publications

Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

Establishment of an efficient in vitro propagation protocol for Sumac (Rhus coriaria L.) and confirmation of the genetic homogeneity

Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

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