Effect of Plant Growth Promoting Bacteria on the Growth of Wheat Seedlings Subjected to Phosphate Starvation

Cataldi, Mariagrazia P.; Heuer, Sigrid; Mauchline, Tim H.; Wilkinson, Mark; Masters-Clark, Emily; Benedetto, N. A. Di; Corbo, Maria Rosaria; Flagella, Zina

doi:10.3390/agronomy10070978

Cited by 11 publications

(5 citation statements)

References 48 publications

(88 reference statements)

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“…Accordingly, the results might suggest that Enterobacter 15S in association with cucumber plants supplemented with Ca 3 (PO 4 ) 2 would have induced a greater P-uptake capacity. Similar behavior was also reported by Cataldi et al ( 2020 ) in the expression levels of TaPHT6 during the interaction of wheat plants with Bacillus strain 12A under P starvation. Concerning the inoculation with the bacteria alone, the results might suggest that Enterobacter 15S could have triggered the different gene regulation, including the modulation of molecular entities other than PHT1 members.…”

Section: Discussionsupporting

confidence: 89%

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

et al. 2021

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Phosphorus (P) is an essential nutrient for plants. The use of plant growth-promoting bacteria (PGPB) may also improve plant development and enhance nutrient availability, thus providing a promising alternative or supplement to chemical fertilizers. This study aimed to evaluate the effectiveness of Enterobacter sp. strain 15S in improving the growth and P acquisition of maize (monocot) and cucumber (dicot) plants under P-deficient hydroponic conditions, either by itself or by solubilizing an external source of inorganic phosphate (Pi) [Ca3(PO4)2]. The inoculation with Enterobacter 15S elicited different effects on the root architecture and biomass of cucumber and maize depending on the P supply. Under sufficient P, the bacterium induced a positive effect on the whole root system architecture of both plants. However, under P deficiency, the bacterium in combination with Ca3(PO4)2 induced a more remarkable effect on cucumber, while the bacterium alone was better in improving the root system of maize compared to non-inoculated plants. In P-deficient plants, bacterial inoculation also led to a chlorophyll content [soil-plant analysis development (SPAD) index] like that in P-sufficient plants (p < 0.05). Regarding P nutrition, the ionomic analysis indicated that inoculation with Enterobacter 15S increased the allocation of P in roots (+31%) and shoots (+53%) of cucumber plants grown in a P-free nutrient solution (NS) supplemented with the external insoluble phosphate, whereas maize plants inoculated with the bacterium alone showed a higher content of P only in roots (36%) but not in shoots. Furthermore, in P-deficient cucumber plants, all Pi transporter genes (CsPT1.3, CsPT1.4, CsPT1.9, and Cucsa383630.1) were upregulated by the bacterium inoculation, whereas, in P-deficient maize plants, the expression of ZmPT1 and ZmPT5 was downregulated by the bacterial inoculation. Taken together, these results suggest that, in its interaction with P-deficient cucumber plants, Enterobacter strain 15S might have solubilized the Ca3(PO4)2 to help the plants overcome P deficiency, while the association of maize plants with the bacterium might have triggered a different mechanism affecting plant metabolism. Thus, the mechanisms by which Enterobacter 15S improves plant growth and P nutrition are dependent on crop and nutrient status.

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Section: Discussionsupporting

confidence: 89%

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

et al. 2021

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“…However, spatial differentiation of microbes in the rhizosphere has been demonstrated to reduce plant-microbe competition as much as possible (Marschner et al, 2011). Additionally, taxonomically and functionally similar bacteria to those we isolated in this study have been shown to increase plant growth when inoculated in soils (Assainar et al, 2018;Masters-Clark et al, 2020;Wang et al, 2020;Wilkinson, 2020).…”

Section: Discussionmentioning

confidence: 53%

Inorganic Chemical Fertilizer Application to Wheat Reduces the Abundance of Putative Plant Growth-Promoting Rhizobacteria

et al. 2021

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The profound negative effect of inorganic chemical fertilizer application on rhizobacterial diversity has been well documented using 16S rRNA gene amplicon sequencing and predictive metagenomics. We aimed to measure the function and relative abundance of readily culturable putative plant growth-promoting rhizobacterial (PGPR) isolates from wheat root soil samples under contrasting inorganic fertilization regimes. We hypothesized that putative PGPR abundance will be reduced in fertilized relative to unfertilized samples. Triticum aestivum cv. Cadenza seeds were sown in a nutrient depleted agricultural soil in pots treated with and without Osmocote® fertilizer containing nitrogen-phosphorous-potassium (NPK). Rhizosphere and rhizoplane samples were collected at flowering stage (10 weeks) and analyzed by culture-independent (CI) amplicon sequence variant (ASV) analysis of rhizobacterial DNA as well as culture-dependent (CD) techniques. Rhizosphere and rhizoplane derived microbiota culture collections were tested for plant growth-promoting traits using functional bioassays. In general, fertilizer addition decreased the proportion of nutrient-solubilizing bacteria (nitrate, phosphate, potassium, iron, and zinc) isolated from rhizocompartments in wheat whereas salt tolerant bacteria were not affected. A “PGPR” database was created from isolate 16S rRNA gene sequences against which total amplified 16S rRNA soil DNA was searched, identifying 1.52% of total community ASVs as culturable PGPR isolates. Bioassays identified a higher proportion of PGPR in non-fertilized samples [rhizosphere (49%) and rhizoplane (91%)] compared to fertilized samples [rhizosphere (21%) and rhizoplane (19%)] which constituted approximately 1.95 and 1.25% in non-fertilized and fertilized total community DNA, respectively. The analyses of 16S rRNA genes and deduced functional profiles provide an in-depth understanding of the responses of bacterial communities to fertilizer; our study suggests that rhizobacteria that potentially benefit plants by mobilizing insoluble nutrients in soil are reduced by chemical fertilizer addition. This knowledge will benefit the development of more targeted biofertilization strategies.

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“…Additionally, inoculation of PGPR increased the amount of N, P, K, and Mg, these PGPR also improved a variety of processes including increased nutrient availability, greater nutrient absorption, and healthier root development. By releasing regular organic acids, PGPR boosts nutritional availability by solubilizing nutrients (Cataldi et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Application of Soil Bacteria as Bioinoculants to Promote Growth of Cowpea (Vigna unguiculata)

et al. 2022

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This work aimed to evaluate the capacity of soil bacteria as bioinoculants (biofertilizers) to promote cowpea (Vigna unguiculata) growth. Three pure bacterial cultures namely Acinetobacter pittii PT1.3.4 (AP), Achromobacter sp.C2.23 (AS), and Achromobacter xylosoxidans N3.4 (AX) were used as bioinoculants to enhance germination and development of cowpea seeds. Pre-decide formulations of single or mixed cultures were prepared, soaked with cowpea seeds, and cultivated on agar in a growth chamber for 7 days at 25°C. Shoot and root length were measured and percentage germination was determined. Similarly, bacterial formulations were prepared in talcum powder and were used as bioinoculants to adhere to cowpea seeds. The inoculated seeds were cultivated in pots for 28 days for the shoot and root length, fresh and dry weight, and percentage germination. Among the tested various formulations, treatment has A. pittii (AP) displayed the highest shoot length (14.67 cm) and fresh weight (0.58 g/plant) of cowpea under laboratory conditions after seven days of inoculation. Similarly, cowpea plants treated with A. pittii (AP) also have the tallest shoots (14.25 cm) under natural conditions after 7 days of inoculation, while the highest root length (10.5 cm) and fresh weight (1.57 g/plant) were recorded from the treatment of Achromobacter sp. (AS). Further, the results of the study also revealed that soil bacteria can survive for one month in talcum powder at 4°C and room temperature storage. These bioinoculants can be used for agricultural application by local farmers to mitigate the cost of chemicals that cause environmental concerns to promote sustainable agriculture in Thailand.

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Effect of Plant Growth Promoting Bacteria on the Growth of Wheat Seedlings Subjected to Phosphate Starvation

Cited by 11 publications

References 48 publications

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

Inorganic Chemical Fertilizer Application to Wheat Reduces the Abundance of Putative Plant Growth-Promoting Rhizobacteria

Application of Soil Bacteria as Bioinoculants to Promote Growth of Cowpea (Vigna unguiculata)

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