Effect of plane of nutrition in early life on the transcriptome of visceral adipose tissue in Angus heifer calves

Keogh, Kate; Kelly, A. K.; Kenny, David A.

doi:10.1038/s41598-021-89252-x

Cited by 12 publications

(11 citation statements)

References 63 publications

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“…Unlike other adipose tissues, IMF deposition in animals occurs mainly from the fetal stage to weaning age. The differentiation of intramuscular preadipocytes mainly occurs in the fetal stage, while the lipid accumulation of intramuscular adipocytes mainly occurs from birth to the weaning age [6][7][8]. After weaning, the content of IMF is usually increased by means of enhanced nutrition and management, but it also results in the accumulation of visceral and subcutaneous fat, which can be detrimental to the health of the animal.…”

Section: Introductionmentioning

confidence: 99%

A genome-wide landscape of mRNAs, lncRNAs, circRNAs and miRNAs during intramuscular adipogenesis in cattle

Yang

Mei

et al. 2022

BMC Genomics

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Background Intramuscular preadipocyte differentiation plays a critical role in bovine intramuscular fat (IMF) deposition. However, the roles of different RNAs, including mRNAs, circRNAs, lncRNAs and miRNAs, in regulating the adipogenic differentiation of intramuscular preadipocytes remain largely unclear. Results In the present study, a whole transcriptome sequencing and analysis, including the analysis of mRNAs, circRNAs, lncRNAs and miRNAs, during different differentiation stages (0, 3, 6, and 9 d) of intramuscular preadipocytes from Qinchuan cattle was performed. All samples were prepared with 3 biological replicates. Here, a total of 27,153 mRNAs, 14,070 circRNAs, 7035 lncRNAs, and 427 miRNAs were annotated. Among them, we identified 4848 differentially expressed mRNAs (DEMs), 181 DE circRNAs (DECs), 501 DE lncRNAs (DELs) and 77 DE miRNAs (DEmiRs) between 0 d and other differentiation days (3, 6, and 9 d). GO and KEGG functional enrichment analyses showed that these differentially expressed genes were mainly enriched in cell differentiation, fat metabolism and adipogenesis-related pathways. Furthermore, weighted gene coexpression network analysis (WGCNA) and co-expression network analysis screened out multiple important mRNAs, circRNAs and lncRNAs related to intramuscular adipogenesis. Based on the competing endogenous RNA (ceRNA) regulatory mechanism, we finally identified 24 potential ceRNA networks and 31 potential key genes, including FOXO1/miR-330/circRNA2018/MSTRG.20301, GPAM/miR-27b/ciRNA489 and SESN3/miR-433/circRNA2627MSTRG.20342. Conclusions This study provides new insights into the differential expression patterns of different transcript types (i.e., mRNAs, circRNAs, lncRNAs and miRNAs) in intramuscular preadipocyte differentiation. Our findings provide data support for studying the molecular mechanism of key mRNAs and noncoding RNAs in IMF deposition, and provide new candidate markers for the molecular breeding of beef cattle.

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Section: Introductionmentioning

confidence: 99%

A genome-wide landscape of mRNAs, lncRNAs, circRNAs and miRNAs during intramuscular adipogenesis in cattle

Yang

Mei

et al. 2022

BMC Genomics

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“…The two key genes ( SDHA and AKT1 ) associated with high nutrition diet mainly play a role in consuming excess energy through mitochondrial respiration and thermogenesis ( Table 1 ; Supplementary Figure 8 ). Previous studies have also shown that they were differentially expressed genes between high and low nutrition diet groups, and participate in the Sirtuin signaling pathway that involved in energy metabolism ( 10 ). As a milk protein related to mitochondrial oxidative metabolism, SDHA was proposed as a putative biomarker of negative energy balance based on its implication in metabolic adaptative pathways ( 48 ).…”

Section: Discussionmentioning

confidence: 97%

“…A total of 29 Angus × Holstein-Friesian heifer calves with a mean age of 19 (±4) days and bodyweight of 51.2 (±7.8) kg were retrieved from the dataset PRJNA664093. The calves were assigned to two groups and fed on diets with a high or low plane of nutrition under the same feeding management conditions ( 10 ). The composition and chemical analysis of the offered reconstituted milk replacer and concentrate were provided in detail in the previous study ( 23 ).…”

Section: Methodsmentioning

confidence: 99%

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Identification of Key Genes Associated With Early Calf-Hood Nutrition in Subcutaneous and Visceral Adipose Tissues by Co-Expression Analysis

Pan

Yang

et al. 2022

Front. Vet. Sci.

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BackgroundSubstantive evidence has confirmed that nutrition state is associated with health risk and the onset of pubertal and metabolic profile. Due to heterogeneity, adipose tissues in different anatomical positions tend to show various metabolic mechanisms for nutrition. To date, the complicated molecular mechanisms of early calf-hood nutrition on bovine adipose tissue are still largely unknown. This study aimed to identify key genes and functionally enriched pathways associated with early calf-hood nutrition in visceral and subcutaneous adipose tissue.ResultsThe RNA-seq data of visceral and subcutaneous adipose tissues of calves feeding on low and high dietary nutrition for more than 100 days were downloaded and analyzed by weighted gene co-expression network analysis (WGCNA). Two modules that positively associated with a low plane of nutrition diet and two modules with a high plane of nutrition diet were identified in the subcutaneous adipose tissue. The blue and yellow modules, most closely associated with low and high nutrition, were selected for the functional enrichment analysis and exploration of hub genes. The results showed that genes in the blue module were significantly enriched in pathways that related to fat metabolism, reproduction, and cell communication. Genes in the yellow module were enriched in pathways related to fat metabolism, reproduction, cell proliferation, and senescence. Meanwhile, the blue and brown modules in visceral adipose tissue were most closely associated with low and high nutrition, respectively. Notably, genes of the blue module were significantly enriched in pathways related to substance metabolism, and genes in the brown module were significantly enriched in energy metabolism and disease pathways. Finally, key genes in subcutaneous adipose tissue for low nutrition (PLCG1, GNA11, and ANXA5) and high nutrition (BUB1B, ASPM, RRM2, PBK, NCAPG, and MKI67), and visceral adipose tissue for low nutrition (RPS5, RPL4, RPL14, and RPLP0) and high nutrition (SDHA and AKT1) were obtained and verified.ConclusionThe study applied WGCNA to identify hub genes and functionally enriched pathways in subcutaneous and visceral adipose tissue and provided a basis for studying the effect of early calf-hood nutrition on the two adipose tissue types.

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“…The first intramuscular adipocytes are formed at 180 days of pregnancy ( Taga et al., 2011 ). The hyperplasia phase of IMF cells lasts till 250 days old whereas the hyperplasia phases of other fat cells may complete at the early postweaning period (intermuscular and subcutaneous adipocytes), or at the neonatal stage (visceral adipocytes) ( Keogh et al., 2021 ). The chronological variation in adipogenesis creates a chance to stimulate IMF cell formation without an increase in the overall adipose accumulation in cattle, which is called the “marbling window” ( Du et al., 2017 , Du et al., 2013 ).…”

Section: The Formation and Development Of Adipose Tissues In Beef Cattlementioning

confidence: 99%

Main regulatory factors of marbling level in beef cattle

Nguyen

Malau‐Aduli

2021

Veterinary and Animal Science

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The content of intramuscular fat (IMF), that determines marbling levels is considered as one of the vital factors influencing beef sensory quality including tenderness, juiciness, flavour and colour. The IMF formation in cattle commences around six months after conception, and continuously grows throughout the life of the animal. The accumulation of marbling is remarkably affected by genetic, sexual, nutritional and management factors. In this review, the adipogenesis and lipogenesis process regulated by various factors and genes during fetal and growing stages is briefly presented. We also discuss the findings of recent studies on the effects of breed, gene, heritability and gender on the marbling accumulation. Various research reported that feeding during pregnancy, concentrate to roughage ratios and the supplementation or restriction of vitamin A, C, and D are crucial nutritional factors affecting the formation and development of IMF. Castration and early weaning combined with high energy feeding are effective management strategies for improving the accumulation of IMF. Furthermore, age and weight at slaughter are also reviewed because they have significant effects on marbling levels. The combination of several factors could positively affect the improvement of the IMF deposition. Therefore, advanced strategies that simultaneously apply genetic, sexual, nutritional and management factors to achieve desired IMF content without detrimental impacts on feed efficiency in high-marbling beef production are essential.

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Effect of plane of nutrition in early life on the transcriptome of visceral adipose tissue in Angus heifer calves

Cited by 12 publications

References 63 publications

A genome-wide landscape of mRNAs, lncRNAs, circRNAs and miRNAs during intramuscular adipogenesis in cattle

A genome-wide landscape of mRNAs, lncRNAs, circRNAs and miRNAs during intramuscular adipogenesis in cattle

Identification of Key Genes Associated With Early Calf-Hood Nutrition in Subcutaneous and Visceral Adipose Tissues by Co-Expression Analysis

Main regulatory factors of marbling level in beef cattle

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