Effect of piperine and its analogs on pharmacokinetic properties of sorafenib tosylate: Bioanalytical method development and validation

Sorafenib and Piperine co-loaded PLGA nanoparticles: Development, characterization, and anti-cancer activity against hepatocellular carcinoma cell line

“…The HPLC method used ( Tiwari et al, 2020 ) was deemed appropriate for chromatographic separation and simultaneous quantification of SORA and PIP. Calibration curves for both drugs were linear within the specified concentration range (0.25–50 μg/mL).…”

“…A previously reported HPLC with UV-detection was utilized for the simultaneous quantification of SORA and PIP ( Escudero-Ortiz et al, 2015 , Tiwari et al, 2020 ). A reversed-phase C 18 column (SunFire TM columns by Waters®, 5 μm, 4.6 × 250 mm) was connected to a Waters® HPLC system (1500-series controller, Milford, MA, USA).…”

“…Subsequently, the mobile phase compositions were pumped isocratically to the HPLC system at 1 mL/min of flow rate. Simultaneous detection of both drugs was performed at 280 nm UV-detection (isosbestic point for SORA and PIP) ( Tiwari et al, 2020 ). The injection volume was 30 µL and the total run-time was 10 min.…”

“…To maintain sink conditions, 1 mL of fresh release medium (kept at 37 ± 2 °C) was added to the beakers after each sampling. The collected samples were transferred to HPLC vials and 30 µL of each sample was injected into an HPLC-UV system for the simultaneous quantification of SORA and PIP at 280 nm ( Tiwari et al, 2020 ). The percentage of drug released (%DR) was estimated using Eq.…”

“…The bioavailability of curcumin was enhanced by 1.5 times in rats and 20 times in human volunteers when administered with PIP ( Shoba et al, 1998 ). PIP has also been shown to improve the pharmacokinetic properties of SORA, which was likely attributed to its Pgp and/or CYP3A4 inhibitory effect ( Patel et al, 2022 , Tiwari et al, 2020 , Tiwari et al, 2021 ).…”