Effect of Pinus taeda Hydrolyzed Lignin on Biochemical Profile, Oxidative Status, and Semen Quality of Healthy Dogs

Abstract

Sub-fertility represents a frequent challenge in canine reproduction. The use of micronutrients and/or additives was investigated as an approach to improve sperm quality, which are the main constraints on reproduction in canine species. Although some information is available about the effect of daily supplementation with substances presenting antioxidant/antioxidative activity on semen quality, this study aimed to observe the effect of a polyphenolic mix of substances derived from hydroxylation of Pinus taeda lignin (PTHL). For the trial, 40 male dogs were involved, 20 received PTHL for 90 days and 20 were left untreated, serving as a control group. Every 30 days, blood and semen samples were collected and analyzed. The biochemical profile of both groups was not affected by treatment and time (p > 0.05). Differently, dogs that received PTHL showed higher blood superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity (p < 0.01). Moreover, the dietary addition of PTHL can significantly increase the semen volume, concentration, and spermatozoa motility (p < 0.01) in healthy dogs. PTHL supplementation represents a good way to enhance the semen quality of dogs and improve the antioxidant status of animals.

Dietary Strategies for Relieving Stress in Pet Dogs and Cats

“…Resveratrol, a natural phytoalexin contained in wine, can reduce the level of ROS and MDA, improve the activities of SOD, GPX, and CAT activities, and improve the ratio of reduced glutamate to oxidized glutamate in cat models in which hepatotoxicity was induced [ 149 ]. Pinus taeda hydrolyzed lignin is a polyphenol mixture that can increase the activity of SOD, CAT, and GPx to improve antioxidant capacity in healthy dogs [ 107 ]. Curcumin extracted from curcuma longa can also enhance total antioxidant capacity by improving the activities of ROS, CAT, SOD, and GPx, and relieve inflammation by reducing lymphocytes and globulin level in dogs [ 108 ].…”

Effects of Dietary Supplementations of Vitamin C, Organic Selenium, Betaine, and Pomegranate Peel on Alleviating the Effect of Heat Stress on Growing Rabbits

“…Serum was separated and stored at −20 °C until analysis. Total protein, albumin, globulin, cholesterol, triglycerides, creatinine, and urea were determined [ 46 ], while globulin was calculated as the difference between total protein and albumin [ 47 ].…”

Effects of Betaine Supplementation on Heat Stress-associated Blood Metabolites, Antioxidant Profiles and Milk Production in Dairy Cows

“…Internal accuracy was verified using two multi-parameter control sera (Seracontrol N and Seracontrol P, Gesan Production Kit, Campobello di Mazara, Trapani, Italy). Each sample was analyzed three times, and the raw dataset recorded the arithmetic mean of the three measurements for each parameter [29]. The NEFA level was determined using diagnostic kits on photometric systems (DIA Lab, Austria), while the BHBA level was analyzed using diagnostic kits from POINTE Scientific INC, USA.…”