Effect of Phytohormones and Group Selective Reagents on Acid Phosphatase from Cladosporium cladosporioides

El-Shora, Hamed M.; Metwally, Mohamed M.M.

doi:10.3923/ajbkr.2009.1.11

Cited by 6 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The four thiol compounds cysteine, DTT, thioglycolate and GSH activated ADI from P. chrysogenum with different levels. DTT was reported to protect the loss of enzyme activity which occurs by the oxidation of sulfhydryl groups during incubation time [ 31 ]. Also, this thiol compound may cause a lowering of the K m of the enzyme to its substrate thereby activating the enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Modified Czapek Dox liquid medium was prepared in 250 ml Erlenmeyer flasks each containing 50 ml (pH 6.0) [ 22 ] using 0.7 g 100 ml − 1 of glucose as carbon source and 0.5 g 100 ml − 1 of yeast extract as nitrogen source. After sterilization, the flasks were inoculated with two ml of spore suspension at a concentration of 10 7 spores ml − 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Detection of the –SH group in purified ADI was carried out according to El-Shora and Metwally [ 31 ]. The purified enzyme (2.5 Uml − 1 ) was incubated at 25 o C for 1 h in 100 mM Tris-HCl (pH 5.0), and 1 mM EDTA with various NEM concentrations (2, 4, 6, 8, and 10 mM).…”

Section: Methodsmentioning

confidence: 99%

“…Detection of the tryptophanyl group was done by the method of [ 31 ]. Aliquot from 2, 4, 6, 8, and 10 mM of NBS was added to 10 ml of ADI enzyme in 150 mM phosphate buffer (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

“…Detection of the histidyl group was carried out according to El-Shora and Metwally [ 31 ]. The solution of DEPC was freshly prepared.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Purification and characterization of L-arginine deiminase from Penicillium chrysogenum

El-Shora,

El-Zawawy,

El-Rheem

et al. 2024

BMC Microbiol

Self Cite

View full text Add to dashboard Cite

L-arginine deiminase (ADI, EC 3.5.3.6) hydrolyzes arginine to ammonia and citrulline which is a natural supplement in health care. ADI was purified from Penicillium chrysogenum using 85% ammonium sulfate, DEAE-cellulose and Sephadex G200. ADI was purified 17.2-fold and 4.6% yield with a specific activity of 50 Umg− 1 protein. The molecular weight was 49 kDa. ADI expressed maximum activity at 40oC and an optimum pH of 6.0. ADI thermostability was investigated and the values of both t0.5 and D were determined. Kd increased by temperature and the Z value was 38oC. ATP, ADP and AMP activated ADI up to 0.6 mM. Cysteine and dithiothreitol activated ADI up to 60 µmol whereas the activation by thioglycolate and reduced glutathione (GSH) prolonged to 80 µmol. EDTA, α,α-dipyridyl, and o-phenanthroline inactivated ADI indicating that ADI is a metalloenzyme. N-ethylmaleimide (NEM), N-bromosuccinimide (NBS), butanedione (BD), dansyl chloride (DC), diethylpyrocarbonate (DEPC) and N-acetyl-imidazole (NAI) inhibited ADI activity indicating the necessity of sulfhydryl, tryptophanyl, arginyl, lysyl, histidyl and tyrosyl groups, respectively for ADI catalysis. The obtained results show that ADI from P. chrysogenum could be a potential candidate for industrial and biotechnological applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Detection of the tryptophanyl group was done by the method of [ 31 ]. Aliquot from 2, 4, 6, 8, and 10 mM of NBS was added to 10 ml of ADI enzyme in 150 mM phosphate buffer (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

“…Detection of the histidyl group was carried out according to El-Shora and Metwally [ 31 ]. The solution of DEPC was freshly prepared.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Purification and characterization of L-arginine deiminase from Penicillium chrysogenum

El-Shora,

El-Zawawy,

El-Rheem

et al. 2024

BMC Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

Essential groups and stability of α-glucosidase ofPenicillium notatum

2009

Self Cite

View full text Add to dashboard Cite

--Glucosidase ( -D-glucoside glucohydrolase, EC 3.2.1.20) was isolated from Penicillium notatum. The enzyme was induced by gibberellic acid (GA 3 ). The GA 3 -mediated increase in the enzyme activity was repressed in presence of abscisic acid, cycloheximide and the antibiotics chloramphenicol, cordycepin, and rifampicin which are inhibitors of protein synthesis. -Glucosidase was purified 440-fold with of 27.8-fold of purification. The enzyme was immobilized using chitosan gel. The optimal pH values were 6.5 and 7.5 for free and the immobilized enzymes, respectively. The optimal temperatures were 50 and 65 °C for free and immobilized enzymes, respectively. The enzyme hydrolyzed maltose, sucrose, isomaltose, maltotriose but not starch, amylopectin and amylose. Trehalose and glycerol protected the free and immobilized enzymes against inactivation at 70 °C, however trehalose was the better protector. Phytate protected the free enzyme against heat inactivation at both 65 and 70 °C. 2,4,6 Trinitrobenzenesulfonic acid, butanedione and diethylpyrocarbonate inactivated the enzyme and suggest that, lysyl, arginyl and histidyl groups are taking part in enzyme catalysis. The inactivation by the three compounds was protected by the substrate para-nitrophenyl--D-glucopyranoside. Treatment of the enzyme with 1-ethyl-3(3-dimethyl aminopropyl)-carbodiimide, p-chloromercuribenzoate, N-bromosuccinimide, N-acetlyimidazole revealing the involving of carboxyl, sulfhydryl, trptophenyl and tyrosyl groups, respectively in the catalysis of -glucosidase. EDTA, o-phenanthroline, dipyridyl and 8-quinolinol inhibited the enzyme activity and the inhibition was higher in case of free enzyme compared with immobilized enzyme.

show abstract

Identification of GR and TrxR systems in Setaria cervi: Purification and characterization of glutathione reductase

Yadav

Elesela

Singh

et al. 2013

Parasitology International

View full text Add to dashboard Cite

Effect of Phytohormones and Group Selective Reagents on Acid Phosphatase from Cladosporium cladosporioides

Cited by 6 publications

References 40 publications

Purification and characterization of L-arginine deiminase from Penicillium chrysogenum

Purification and characterization of L-arginine deiminase from Penicillium chrysogenum

Essential groups and stability of α-glucosidase ofPenicillium notatum

Identification of GR and TrxR systems in Setaria cervi: Purification and characterization of glutathione reductase

Contact Info

Product

Resources

About