Effect of physical parameters, carbon and nitrogen sources on the production of alkaline protease from a newly isolatedBacillus pseudofirmus SVB1

Sen, Shampa; Dasu, Veeranki Venkata; Mandal, Bishnupada

doi:10.1007/bf03175142

Cited by 30 publications

(15 citation statements)

References 37 publications

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“…Several growth factors affect the productivity and growth of micro-organisms particularly substrate particle size is one of the most critical parameter [25]. It has been reported in lit-erature that low cost substrates like wheat flour, wheat bran, rice straws and molasses are suitably effective for growth and enzyme production [26,27]. Optimization of various environmental factors causes an increase of enzymatic activities [28].…”

Section: Production Of Cellulasementioning

confidence: 99%

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Iqbal¹,

Ahmed²,

Zia³

et al. 2011

ABB

108

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This paper reports the purification and characterization of kinetic parameters of cellulase produced from Trichoderma viride under still culture solid state fermentation technique using cheap and an easily available agricultural waste material, wheat straw as growth supported substrate. Trichoderma viride was cultured in fermentation medium of wheat straw under some previously optimized growth conditions and maximum activity of 398 ± 2.43 U/mL obtained after stipulated fermentation time period. Cellulase was purified 2.33 fold with specific activity of 105 U/mg in comparison to crude enzyme extract using ammonium sulfate precipitation, dialysis and Sephadex-G-100 column chromatography. The enzyme was shown to have a relative low molecular weight of 58 kDa by sodium dodecyl sulphate poly-acrylamide gel electrophoresis. The purified enzyme displayed 6.5 and 55˚C as an optimum pH and temperature respectively. Using carboxymethyl cellulose as substrate, the enzyme showed maximum activity (V max ) of 148 U/mL with its corresponding K M value of 68 µM. Among activators/inhibitors SDS, EDTA, and Hg 2+ showed inhibitory effect on purified cellulase whereas, the enzyme activated by Co 2+ and Mn 2+ at a concentration of 1 mM. The purified cellulase was compatible with four local detergent brands with up to 20 days of shelf life at room temperature suggesting its potential as a detergent additive for improved washing therefore, it is concluded that it may be potentially useful for industrial purposes especially for detergent and laundry industry.

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Section: Production Of Cellulasementioning

confidence: 99%

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Iqbal¹,

Ahmed²,

Zia³

et al. 2011

ABB

108

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“…Several Bacillus strains such as Bacillus caldoproteolyticus [42], Bacillus clausii [43], Bacillus subtilis [44], Bacillus karajensis [45], Bacillus sp haericus [46], Bacillus pseudofirmus [47], Bacillus tequilensis [48] and Haloalkaliphilic bacterium sp [49] Moderate halophile Halomonas heilongjiangensis [50]. Borderline extreme halophile Halosiccatus urmianus [51].…”

Section: Enzymesmentioning

confidence: 99%

An Overview of Saltpan Halophilic Bacterium

Manikandan¹,

Pk²

2017

J Antimicrob Agents

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Hypersaline environments provide an excellent medium for natural microbial communities which serve as a potential source of pharmaceutical substances. Salt is widely present in the earth. Almost 73% of earth was covered with marine water which contains 2.5% of common salt. Protease enzyme activity widespread in microorganisms, plant and animals. Proteolytic enzymes used in the industrial application and bioremediation process. In recent years' new mutant's microbe resistant to commonly used antibiotics. Protease inhibitors used as potential antibiotics for controlling microbial infections. A Hypersaline environment such as salt pans and salt lakes has high salt concentration and pH. The saltpan provides a diversity of different environmental conditions of alkalinity, salinity, temperature, pH and nutrition. Halophilic organisms growing between 0.5 and 3.0 M salt concentration. Extreme environments are the best source of bioactive compound producing halophiles microbes.

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“…Particle size is considered to be the most critical factor among other growth factors, and greatly influenced the growth and enzyme yields of micro-organisms (Zadrazil and Puniya 1995). The previous literature illustrates that some low-cost agro-industrial substrates (molasses, rice straw, wheat bran, and flour) have a significant effect on production and maximally enhance enzyme yield (Mehta et al 2006;Sen et al 2009). Ojumu et al (2003) found that saw dust, corn cob, and bagasse agro substrates treated with 3% HCl increase cellulase activity at a maximum level.…”

Section: Production Of Endoglucanasementioning

confidence: 99%

Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation

2016

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A robust process of purification, characterization, and application of endoglucanase from the agro-industrial waste was performed using solid state fermentation (SSF). Trichoderma harzianum as a micro-organism and wheat straw as a growth supportive substrate were used in SSF under pre-optimized conditions. The maximum activity of 480 ± 4.22 U/mL of endoglucanase was attained when a fermentation medium was inoculated using 10% inoculum size and 3% substrate concentration with pH = 5.5 at 35 °C for an optimized fermentation period. In comparison with crude extract, enzyme was 1.83-fold purified with a specific activity of 101.05 U/mg using Sephadex-G-100 column chromatography. Sodium dodecyl sulfate (SDS) poly-acrylamide gel electrophoresis revealed that the enzyme exhibited a low molecular weight of 43 kDa. The purified enzyme displayed maximum activity at pH = 6 and a temperature of 50 °C, respectively. The maximum activity (Vmax) of 156 U/mL and KM value of 63 µM were observed. Ethylenediaminetetraacetic acid (EDTA), SDS, and Hg 2+ inhibited enzyme activity, while Co 2+ and Mn 2+ enhanced enzyme activity at 1 mM concentration. The maximum substrate affinity and specific activity of biosynthesized endoglucanase revealed that it can be potentially useful for industrial applications.

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Effect of physical parameters, carbon and nitrogen sources on the production of alkaline protease from a newly isolatedBacillus pseudofirmus SVB1

Cited by 30 publications

References 37 publications

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility

An Overview of Saltpan Halophilic Bacterium

Detergent-Compatible Purified Endoglucanase from the Agro-Industrial Residue by Trichoderma harzianum under Solid State Fermentation

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