Effect of Physical, Chemical and Physiochemical Treatments of Surface Sterilisation on Medicinal Plants Salvadora persica and Solanum surattense for In-vitro Propagation

Abstract

Effect of various surface sterilisation treatments on medicinal plants Salvadora persica andSolanum surattense has been studied for in-vitro propagation. Surface sterilisation treatment was appliedby physical, chemical and physiochemical means. Chemical sterilisation was done with sodium hypochlorite,physically with sonication and physiochemically with combination of both. Sodium hypochlorite alonewas found to be effective for smooth textured plant i.e., Salvadora persica, whereas for rough texturedplant Solanum surattense a combination of both physiochemical means yielded good results. However,sonication alone did not render the effective way of surface sterilisation. Moreover, for Salvadora persica,10% sodium hypochlorite was effective in eliminating bacterial or fungal growth whereas in Solanumsurattense, a concentration of 15% sodium hypochlorite proved to be effective. In general, best resultswere achieved in the combined treatment by physiochemical means.