Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells

Abstract

Abstract. The present study assessed the effect of photodynamic therapy (PDT) combined with Celecoxib (Cel) on cervical cancer HeLa cells. An MTT assay was performed to detect the inhibitory effects of Cel with different concentrations (10, 50, 100, 150, 200, 250 and 300 µg/ml) on the proliferation of HeLa cells. Subsequently, HeLa cells were divided into control group (group H), 50 g/ml Celecoxib group (group C), PDT group (group P), 50 g/ml Cel + PDT group (group P + C) and western blotting and immunohistochemistry were performed to detect the expression of cyclooxygenase-2 (COX-2) protein in the different groups. Cel inhibited HeLa cells proliferation 24 h following administration, among which 200 µg/ml induced a 50% inhibition rate; the relative expression level of COX-2 protein in group P + C was significantly decreased compared with that in either group C or group P (P<0.05). Cel inhibited the proliferation of human cervical cancer cells in a concentration-dependent manner, and combined PDT therapy may improve treatment outcomes.

High-Throughput Screening of Lipidomic Adaptations in Cultured Cells

“…COX-2 is best known as an inducible protein, expressed at sites of inflammation, infection, and cancer [28,29]. However, constitutive expression of COX-2 also occurs in various organs, and activity of COX-2 in HeLa cells has been reported [30,31]. By its action, celicoxib will reduce the conversion of arachidonic acid to prostanoids and thus can be expected to increase arachidonic acid levels in the phospholipidome.…”

Gliclazide Prevents 5-FU-Induced Oral Mucositis by Reducing Oxidative Stress, Inflammation, and P-Selectin Adhesion Molecules

“…Another important marker of the inflammatory process is the cyclooxygenase-2 (Cox-2), which has direct participation in the formation of the prostaglandins involved in the inflammatory process (Gariepy et al, 2017; Mao et al, 2018). Inhibition of Cox-2 is recognized in the success of therapeutic interventions for OM induced by chemotherapy (Sommer et al, 2017; Mahendran et al, 2018).…”

The use of photodynamic therapy in medical practice

“…Another drug, ripasudil, an inhibitor of Rho kinase (ROCK), a GTPase that regulates the organization of the actin cytoskeleton, enhances phagocytic activity and the ability to process antigens by antigen-presenting cells (APCs), and in additional combination with an anti-PD-1 drug and photodynamic therapy with a nanophotosensitizer embedded in Ce6 (FIC-PDT) causes a systemic, long-lasting anti-tumor response of melanoma cells ( 284 ). The combination of PDT with the COX-2 inhibitor (isoenzyme of normal cyclooxygenase, found, among others, in cervical cancer cells) - celocoxib, significantly improves treatment results ( 285 ).…”

“…In patients with periodontitis with rapid progression and severe severity, photodynamic therapy improves the clinical condition, but should not be used as a substitute for amoxicillin and metronidazole (275). PDT causes a significant reduction of bacteria, but their complete elimination is usually not achieved (276)(277)(278)(279)(280)(281)(282)(283)(284)(285)(286)(287). It is also important to use the most selective photosensitizers possible, because killing the entire physiological flora of the oral cavity exposes patients to infections with opportunistic pathogens (276).…”