Objective: The rapid palatal expansion (RPE) procedure is a
frequently used treatment for malocclusion before pubertal growth.
However, relapses often occur after the retention period of this
procedure. In this study, we aimed to eliminate this deficiency,
considering that there would be a deficiency in the new bone metabolism
in the expanded area between the palatal sutures. Design:
Twenty-four male Wistar rats, 7-8 weeks old, with an average weight of
200 g, were divided into 3 groups in equal numbers. 5 days of expansion
was applied to all groups with 50 g of force, then a retention period of
12 days was expected. While no application was made to the control group
(CG) during the retention period, mechanical vibration was applied to
the vibration group (VF) at a frequency of 111 Hz and the active
substance was applied to the thymoquinone group (TQ) at a dose of 10
mg/kg. Result: At the end of the retention period, all animals
were sacrificed and sections were taken from the premaxillary region and
histological evaluation was made in terms of osteoblast, osteoclast,
capillary numbers, amount of mineralized and fibrous areas. According to
the statistical analysis, TQ and VF showed more positive and similar
results than CG in terms of new bone formation for all histomorphometric
parameters except osteoclast numbers in the comparison of parameters in
all groups (p<0.05). Conclusions: The new bone
stimulation effects of non-invasive vibration force and Thymoquinone
applications and ease of use can prevent relapse in the RPE.