The experiment was laid out in a factorial randomized block design comprising eighteen treatment combinations of three levels of N (0, 10 and 20 kg ha -1 ), three levels of S (0, 10 and 20 kg ha -1 ) and two levels of biofertilizer (seed bio-priming and without Rhizobium) which were replicated three times. The results indicated that plot with the application of N and S @ 20 kg ha -1 gave significantly (p< 0.05) higher seed yield (~ 32 and 21%) and stover yield (~ 16 and 18%) as compared to control plot, respectively. Bio-primed observed significantly (p < 0.05) higher seed yield (~ 996 kg ha -1 ) and stover yield (~ 1829 kg ha -1 ) as compared to un-inoculated treatments. Meanwhile, plot with the application of N and S @ 20 kg ha -1 + bio-priming @ 25 g kg -1 seeds on loamy sand soil resulted the increased the seed yield ~ 32, 21 and 7%, respectively and nutrients availability besides organic carbon (5, 2 and 2.3%), available N (5, 4.5 and 4.6%), P 2 O 5 (7.5, 2 and 0.5%), and S (12, 22 and 11%, respectively) concentration increased in soil after harvest of green gram under bio-primed treatments.