An experiment had been done in split design with 4 replicates in RasSudr Research Station, Desert Research Center, at South Sinai Governorate, Egypt, through 2009 on four years old Prosopis Chilensis to evaluate the effect of inoculation with Bradyrhizobium japonicum, Azotobacter chroococcum, Bacillus megatherium (PDB) and +VA mycorrhizae singly or in combination with vegetative (plant height, stem diameter and number of branches) and pod production (pod number/plant and pod weight/plant) and some chemical constituents total flavonoid). Results indicated that different biofertilizer treatments and their interactions significantly increased prosopis yield (pod number and pod weight), chemical analysis of leaves and branches (total carbohydrates, digestive protein, total digestive nutrients and total flavonoids) and chemical constituents of pods (crude protein, ash%, total carbohydrate, total sugar, total flavonoid and macro and microelements). Mixed inoculation gave better results than single ones, inoculation treatments can be arranged in descending order as follow, quarto inoculation, triple inoculation, double inoculation double inoculation and finally single inoculation. ª 2014 Production and hosting by Elsevier B.V. on behalf of Faculty of Agriculture, Ain Shams University.