Effect of phosphorus deficiency on acid phosphatase and phytase activities in common bean (Phaseolus vulgaris L.) under symbiotic nitrogen fixation

Kouas, Saber; Louche, Julien; Debez, Ahmed; Plassard, Claude; Drevon, J. J.; Abdelly, Chédly

doi:10.1007/bf03179974

Cited by 35 publications

(17 citation statements)

References 43 publications

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“…In addition to bacterial excreted phosphatases as discussed above, plants and associated nodules can also be substantial contributors of phosphatases (Araujo et al, 2008b;Penheiter et al, 1997b). This activity may contribute to P availability that is essential for rhizobium nodule development and productivity for N 2 -fixing legumes, especially under P limited conditions (Araujo et al, 2008a;Kouas et al, 2009). In the organically-managed soybean fields across PEI, we found a relationship between phosphatase activity in the rhizosphere, nodule weight and plant P uptake.…”

Section: Soil P Availability and Contribution To Plant Growthmentioning

confidence: 97%

Quantification of bacterial non-specific acid ( phoC) and alkaline ( phoD ) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields

Fraser

Lynch

Gaiero

et al. 2017

Applied Soil Ecology

150

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Section: Soil P Availability and Contribution To Plant Growthmentioning

confidence: 97%

Quantification of bacterial non-specific acid ( phoC) and alkaline ( phoD ) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields

Fraser

Lynch

Gaiero

et al. 2017

Applied Soil Ecology

150

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“…The inorganic phosphorous released was quantified by the molybdovanadate method at 460 nm (Kouas et al 2009). One unit of activity was defined as the amount of enzyme resulting in the hydrolysis of 1 μmol of substrate min -1 .…”

Section: Intracellular Acid Phosphatase (Apase) and Phytase Assaysmentioning

confidence: 99%

Legume nodules from nutrient-poor soils exhibit high plasticity of cellular phosphorus recycling and conservation during variable phosphorus supply

Vardien

Steenkamp

Valentine

2016

Journal of Plant Physiology

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Nitrogen fixing legumes rely on phosphorus for nodule formation, nodule function and the energy costs of fixation. Phosphorus is however very limited in soils, especially in ancient sandstone-derived soils such as those in the Cape Floristic Region of South Africa. Plants growing in such areas have evolved the ability to tolerate phosphorus stress by eliciting an array of physiological and biochemical responses. In this study we investigated the effects of phosphorus limitation on N 2 fixation and phosphorus recycling in the nodules of Virgilia divaricata (Adamson), a legume native to the Cape Floristic Region. In particular, we focused on nutrient acquisition efficiencies, phosphorus fractions and the exudation and accumulation of phosphatases. Our finding indicate that during low phosphorus supply, V. divaricata internally recycles phosphorus and has a lower uptake rate of phosphorus, as well as lower levels adenylates but greater levels of phosphohydrolase exudation suggesting it engages in recycling internal nodule phosphorus pools and making use of alternate bypass routes in order to conserve phosphorus.

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“…Similarly, common bean nodules increase APase activity under P deficiency (Kouas et al, 2009). Our findings on V. divaricata therefore agree with many studies reporting that P stress induces APase activity, aiding in the internal recycling of P and the increased APase activity in nodules and roots under P-deficiency, explains the great requirement for P for BNF (Vadez et al, 1997, Al-Niemi et al, 1998).…”

Section: Nutrition Parameter Phosphate Treatmentmentioning

confidence: 99%

Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels

Vardien

Mesjasz‐Przybyłowicz

Przybyłowicz

et al. 2014

Journal of Plant Physiology

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Legumes have the unique ability to fix atmospheric nitrogen (N 2 ) via symbiotic bacteria in their nodules but depend heavily on phosphorus (P), which affects nodulation, and the carbon costs and energy costs of N 2 fixation. Consequently, legumes growing in nutrient-poor ecosystems (e.g. sandstone derived soils) have to enhance P recycling and/ or acquisition in order to maintain N 2 fixation. In this study, we investigated the flexibility of P recycling and distribution within the nodules and their effect on N nutrition in Virgilia divaricata Adamson, Fabaceae, an indigenous legume in the Cape Floristic Region of South Africa. Specifically, we assessed tissue elemental localization using micro-particle induced x-ray emission 1 (PIXE), measured N fixation using nutrient concentrations derived from inductively coupled mass spectrometry (ICP-MS), calculated nutrient costs, and determined P recycling from enzyme activity assays. Morphological and physiological features characteristic of adaptation to P-deprivation were observed for V. divaricata. Decreased plant growth and nodule production with parallel increased root: shoot ratios are some of the plastic features exhibited in response to P deficiency. Plants resupplied with P resembled those supplied with optimal P levels in terms of growth and nutrient acquisition. Under low P conditions, plants maintained an increase in N 2 -fixing efficiency despite lower levels of orthophosphate (Pi) in the nodules.This can be attributed to two factors: i) an increase in Fe concentration under low P, and ii) greater APase activity in both the roots and nodules under low P. These findings suggest that V. divaricata is well-adapted to acquire N under P deficiency, owing to the plasticity of its nodule physiology.

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Effect of phosphorus deficiency on acid phosphatase and phytase activities in common bean (Phaseolus vulgaris L.) under symbiotic nitrogen fixation

Cited by 35 publications

References 43 publications

Quantification of bacterial non-specific acid ( phoC) and alkaline ( phoD ) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields

Quantification of bacterial non-specific acid ( phoC) and alkaline ( phoD ) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields

Legume nodules from nutrient-poor soils exhibit high plasticity of cellular phosphorus recycling and conservation during variable phosphorus supply

Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels

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