Effect of phorbol ester on phosphoinositide hydrolysis and calcium mobilization induced by endothelin-1 in cultured canine tracheal smooth muscle cells

Yang, Che‐Hua; Ong, Richard; Chen, Ying-Cheng; Hsieh, Jen-Tsung; Tsao, Hui Liang; Tsai, Chuen Tao

doi:10.1016/0143-4160(95)90082-9

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…The 1 H and 13 C assignments of GlcNAc-TA are summarized in Table 2. The 1 H and 13 C NMR spectra of GlcNAc-TA showed a mixture of conformers in the ratio of 2 to 1 (conformer-1 : conformer-2) as observed in other teleocidins [4] and the existence of N-acetylglucosamine. The long-range coupling from an anomeric proton (H-1Ј) to a methylene carbon bearing oxygen (C-14) in the HMBC spectrum indicated the connectivity between Nacetylglucosamine and teleocidin A.…”

Section: Physico-chemical Properties and Structural Elucidationsupporting

confidence: 52%

“…Our findings indicate that glucosylated GlcNAc-TA retains activities although it is less potent than teleocidin A. We established that GlcNAc-TA translocats PKCa -EGFP of the conventional PKCs and PKCq-EGFP of novel PKCs to the plasma membrane and confirmed that GlcNAc-TA also inhibits Ca 2ϩ mobilization induced by five agonists of GPCRs at level of 2.1ϳ4.3 mM as well as other activators of PKC which are reported to inhibit the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) and Ca 2ϩ mobilization by agonists of GPCRs like as endothelin-1, bradykinin and carbachol [7,13,14]. These activities are consistent with the known biological properties of teleocidins.…”

Section: Discussion and Summurymentioning

confidence: 65%

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A New Teleocidin Analog from Streptomyces sp. MM216-87F4 Induces Substance P Release from Rat Dorsal Root Ganglion Neurons

et al. 2006

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A new teleocidin analog was isolated from the fermentation medium of Streptomyces sp. MM216-87F4 and its structure was elucidated as 14-O-(Nacetylglucosaminyl) teleocidin A (GlcNAc-TA). GlcNAc-TA induces the translocation of protein kinases Ca and q fused with enhanced green fluorescent protein (PKCa -EGFP and PKCq -EGFP) to the plasma membrane in stable transfectants, and reduces intracellular calcium mobilization induced by agonists of G-protein coupled receptors in various cell lines without causing irritation of the mouse ear. Further, GlcNAc-TA sensitizes the release of excitatory neuropeptides substance P induced by capsaicin from primary-cultured dorsal root ganglion (DRG) neurons of the rat and GlcNAc-TA alone also triggers substance P release in a dose-dependent manner. This study provides the first observation that a teleocidin analog without a free hydroxyl group at C-14 acts as a PKC activator and directly induces the release of excitatory neuropeptide.

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Section: Physico-chemical Properties and Structural Elucidationsupporting

confidence: 52%

Section: Discussion and Summurymentioning

confidence: 65%

A New Teleocidin Analog from Streptomyces sp. MM216-87F4 Induces Substance P Release from Rat Dorsal Root Ganglion Neurons

et al. 2006

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“…Xuan et al 1994;Yang et al 1995), although evidence has been provided that different entry pathways might become activated by ET-1 in parallel (Iwamuro et al 1999). In comparison with conventional receptors converging on the PLC-InsP 3 signalling cascade, ET-1-induced Ca 2+ transients are characterized by their much longer duration and by a lack in reproducibility in an acute experiment.…”

Section: Discussionmentioning

confidence: 96%

Ca2+ entry but not Ca2+ release is necessary for desensitization of ETA receptors in airway epithelial cells

Meyer

Oles

Pott

2000

Naunyn-Schmiedeberg's Arch Pharmacol

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Ca2+ transients evoked by endothelin-1 (ET-1) were measured in single cells of a human tracheal epithelial cell line using the fluorescent Ca2+ indicator fura-2. In line with a previous study, a single exposure to ET-1 (10 nM) for 10-20 s resulted in a long-lasting desensitization to a subsequent challenge by the peptide, without affecting sensitivity to agonists for other Ca2+-mobilizing receptors such as P2y or H1, respectively. In the absence of extracellular Ca2+ ET-1 elicited a Ca2+ signal of comparable amplitude as in the presence of extracellular Ca2+ but of shorter duration. Exposure to ET-1 in the absence of Ca2+ caused significantly less desensitization. Inhibition of the Ca2+ entry component of the Ca2+ transient by means of SK&F 96365, an inhibitor of Ca2+ entry, had effects comparable to Ca2+ removal. The Ca2+ transient was shortened but not significantly reduced in amplitude, and desensitization was reduced in the presence of the compound. These data demonstrate that desensitization of ET(A) receptors (ET(A)R) is promoted by transmembrane Ca2+ entry but not by Ca2+ release.

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ET Receptor-Linked Signal Transduction Processes in the Airway Wall

Henry

1999

Pulmonary Actions of the Endothelins

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Effect of phorbol ester on phosphoinositide hydrolysis and calcium mobilization induced by endothelin-1 in cultured canine tracheal smooth muscle cells

Cited by 8 publications

References 31 publications

A New Teleocidin Analog from Streptomyces sp. MM216-87F4 Induces Substance P Release from Rat Dorsal Root Ganglion Neurons

A New Teleocidin Analog from Streptomyces sp. MM216-87F4 Induces Substance P Release from Rat Dorsal Root Ganglion Neurons

Ca2+ entry but not Ca2+ release is necessary for desensitization of ETA receptors in airway epithelial cells

ET Receptor-Linked Signal Transduction Processes in the Airway Wall

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