Effect of pH, temperature and incubation time on cordycepin production from<i>Cordyceps militaris</i>using solid-state fermentation on various substrates

Adnan, Mohd; Ashraf, Syed Amir; Khan, Saif; Al-Shammari, Eyad; Awadelkareem, Amir Mahgoub

doi:10.1080/19476337.2017.1325406

Cited by 41 publications

(22 citation statements)

References 10 publications

(7 reference statements)

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“…The highest titer of cordycepin was found in the medium broth with xylose at 149.5 ± 15.71 mg/L, whereas titers of 119.32 ± 14.06 mg/L and 109.14 ± 11.54 mg/L were obtained in sucrose and glucose cultures, respectively. Notably, the enhanced extracellular cordycepin titer in xylose culture was obtained by prolonged cultivation (Table 1 ), which is in agreement with a previous report 18 . However, this phenomenon was not found in glucose and sucrose cultures.…”

Section: Resultssupporting

confidence: 92%

Uncovering global metabolic response to cordycepin production in Cordyceps militaris through transcriptome and genome-scale network-driven analysis

Raethong

Laoteng

Vongsangnak

2018

Sci Rep

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The cellular metabolic adaptations of Cordyceps militaris have been progressively studied. In particular, the cordycepin pathway is of interest in medicinal applications. Even though the metabolic pathways for cordycepin production are known to be related to different carbon sources, the regulatory mechanisms at a systems level are poorly characterized. To explore the regulatory mechanisms, this study therefore aimed to investigate the global metabolic response to cordycepin production in C. militaris through transcriptome analysis and genome-scale network-driven analysis. Here, transcriptome analysis of 16,805 expressed genes in C. militaris strain TBRC6039 grown on different carbon sources was performed. Of these genes, 2,883 were significantly differentially expressed genes, uncovering sucrose- and glucose-mediated changes in the transcriptional regulation of central carbon metabolism in C. militaris, which was shown using the CmSNF1 mechanism as an example. After applying genome-scale metabolic network-driven analysis, reporter metabolites and key metabolic subnetworks involving adenosine, cordycepin and methionine were proposed through the up-regulation of cordycepin biosynthetic genes. Our findings suggest that the transcriptional regulation of these pathways is a ubiquitous feature in response to specific culture conditions during cordycepin overproduction.

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Section: Resultssupporting

confidence: 92%

Uncovering global metabolic response to cordycepin production in Cordyceps militaris through transcriptome and genome-scale network-driven analysis

Raethong

Laoteng

Vongsangnak

2018

Sci Rep

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“…In fact, because of the strict requirement for insect hosts and growth environment, most CM are commercially produced with solid‐state fermentation (SSF), submerged fermentation (SF), and surface liquid fermentation (SLF) by artificial cultivation (Adnan, Ashraf, Khan, Alshammari, & Awadelkareem, ). Almost all of the biological active components are extracted from the fermented solution or fruit bodies, while the spent substrate (SS) which was used to cultivate the CM fruit body is rarely used after the CM attained maturity (Sun et al, ; Yang, Wu, He, Zhou, & Yang, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of superfine grinding on the properties and qualities of Cordyceps militaris and its spent substrate

Shi

Zhang

Bhandari

et al. 2019

J Food Process Preserv

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The main purpose of this study is to study the effect of superfine grinding (SG) technology on the physicochemical properties of Cordyceps militaris (CM) and its spent substrate (SS). The results showed that the particle size of the sample powder decreased significantly under the SG treatment and reached below 15 μm finally. With the decrease in particle size, the surface tension of materials decreased and water‐holding capacity (WHC) of aqueous solution of CM and its SS powder decreased by 58.72% and 48.36%, while the water soluble index (WSI) increased to 0.73 and 0.44 g/g, respectively, also the hygroscopic property, L* value and DPPH radical scavenging capacity increased significantly. In addition, the cordycepin yield of two materials increased by 9.49% and 14.61% for 4 hr SG treatment, respectively. The results showed that the SG technology could effectively improve the physicochemical properties of CM powder and the extraction of its effective components, in addition, a new idea was opened up for the effective utilization of waste materials such as edible and medicinal fungi culture medium. Practical applicationsThis study investigated the effect of superfine grinding treatments on the physicochemical properties of the powder of Cordyceps militaris (CM) and its spent substrate (SS). The results shows the advances of superfine grinding on the particle size, surface tension, water hydration capacity, color, antioxidant capacity, and the yield of active component. And it could realize the maximum utilization of resources like medical plants and the reutilization of the waste during herb culturing, when treatments like superfine grinding was applied.

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“…28 Thus, the variation of pH may reflect the changes in nutrient consumption or even metabolic reactions in substrates during SSF. 29 High contents of crude and soluble proteins were obtained in the NS-FSBM after fermentation, which illustrated that the SSF using B. stearothermophilus with non-sterile SBM could achieve better effects on the production of crude and soluble proteins compared to sterile SBM. The results mentioned earlier further showed that B. stearothermophilus could decompose and consume carbohydrates and increase the relative crude protein content in FSBM.…”

Section: Discussionmentioning

confidence: 80%

“…The pH is a crucial process parameter that reflects the metabolic activity among microorganisms under certain conditions . Thus, the variation of pH may reflect the changes in nutrient consumption or even metabolic reactions in substrates during SSF …”

Section: Discussionmentioning

confidence: 99%

Feasibility study on direct fermentation of soybean meal by Bacillus stearothermophilus under non‐sterile conditions

Guo

Golly

et al. 2019

J Sci Food Agric

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BACKGROUND To evaluate the feasibility of high‐temperature solid‐state fermentation (SSF) using soybean meal (SBM) during the non‐sterile process, Bacillus stearothermophilus was employed to assess the nutritional quality and bioactivity of SBM after fermentation. RESULTS The fermented SBM (FSBM) without autoclaving showed significant improvements in nutritional quality and bioactivity. The contents of peptides and crude and soluble proteins increased by 131.21%, 5.3% and 15.52%, respectively. Meanwhile, DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) scavenging ability, reducing ability and hydroxyl free radical‐scavenging activity rose by 57.07%, 238.92% and 368.26%, respectively. The inhibitory activity of angiotensin I‐converting enzyme increased from 1.43 ± 0.83% to 26.89 ± 1.03%, while the trypsin inhibitor activity decreased by 74.05%. The contents of neutral and alkaline proteases and the growth of microorganisms in FSBM without autoclaving were higher and better than in steam‐treated FSBM. After steam treatment, the water‐holding capacity of SBM decreased, and a high crosslink density was observed on the surface of SBM particles. CONCLUSIONS It is feasible to ferment SBM by high‐temperature SSF using B. stearothermophilus under non‐sterile conditions. Adverse effects of SSF using sterile SBM might be owing to the low water‐holding capacity caused by autoclaving. © 2018 Society of Chemical Industry

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Effect of pH, temperature and incubation time on cordycepin production fromCordyceps militarisusing solid-state fermentation on various substrates

Cited by 41 publications

References 10 publications

Uncovering global metabolic response to cordycepin production in Cordyceps militaris through transcriptome and genome-scale network-driven analysis

Uncovering global metabolic response to cordycepin production in Cordyceps militaris through transcriptome and genome-scale network-driven analysis

Effects of superfine grinding on the properties and qualities of Cordyceps militaris and its spent substrate

Feasibility study on direct fermentation of soybean meal by Bacillus stearothermophilus under non‐sterile conditions

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