Effect of pH, temperature and diet on digestive enzyme profiles in the mud crab, Scylla serrata

Pavasovic, Marko; Richardson, Neil; Anderson, Alan J.; Mann, David L.; Mather, Peter B.

doi:10.1016/j.aquaculture.2004.08.036

Cited by 84 publications

(70 citation statements)

References 21 publications

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“…However, because snail meat was the only diet provided for E. sinensis during the entire experimental period, high amylase and cellulose enzyme activities could not related to the present diets, but was rather attributed to the previous secretion before reproductive migration in the river and lakes where adult crabs fed mainly on hydrophytes. Similar to the present results, cellulose activity has been detected in a wide range of aquatic invertebrates whose diets do not normally include substantial amounts of cellulose (Elyakova et al 1981, Johnston 2003, Pavasovic et al 2004. As proposed by Yokoe and Yasumasu (1964), the distribution of cellulose within the animal kingdom was more closely correlated with animal phylogenetic relationships than with differences and similarities in their feeding habits.…”

Section: Discussionsupporting

confidence: 90%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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SUMMARY: Mature Chinese mitten crabs, Eriocheir sinensis, were exposed to brackish water or seawater as an obligatory part of their reproductive migration. Physiological and biochemical reorganization were needed to adapt them to this migration. To understand the digestive adjustments of Eriocheir sinensis at biochemical level during this transformation from freshwater to seawater, the response of the activity of five digestive enzymes (amylase, cellulase, pepsin, trypsin and lipase) in the hepatopancreas to salinities increasing gradually from 0 (freshwater) to 35 (seawater) was analysed in mature females and males. Digestive enzymes exhibited significantly higher activities in the hepatopancreas of males than those of females, except lipase. In females, amylase, pepsin and trypsin activities began to decrease significantly as the salinity reached 28, and cellulase activity decreased at 35; in males, a considerable decrease in the activity of digestive enzymes, except lipase, was observed at 21 and higher salinities, while an increase was observed at 14. Reduced enzyme activities at elevated salinities suggest that the digestive capacity of crabs for diets becomes weak, and all these digestive enzymes participated in digestive adjustments during osmoregulation. The initial salinity which induced the decrease of enzyme activity was lower in males than in females, indicating that females were more tolerant to elevated salinities than males from the point of digestive biochemical modulation.Keywords: mature crab, digestive enzyme, sexual difference, metabolic regulation, osmoregulation, salinity. RESUMEN: Respuesta de la actividad enzimática digestiva al incRemento gRadual de la salinidad en el cangRejo de shanghai maduRo, EriochEir sinEnsis (decapoda: BRachyuRa). -Se expusieron cangrejos de Shanghai maduros (Eriocheir sinensis) a agua salobre o agua marina obligatoriamente durante la migración reproductora. A fin de que los ejemplares se adaptaran a esta migración, fue preciso proceder a una reorganización fisiológica y bioquímica. Con objeto de estudiar los ajustes digestivos de carácter bioquímico del Eriocheir sinensis durante la transformación del agua dulce en agua marina, se analizó la reacción de la actividad en el hepatopáncreas de cinco enzimas digestivas (amilasa, celulasa, pepsina, tripsina y lipasa) a un aumento gradual de la salinidad, desde 0 ppt (agua dulce) hasta 35 ppt (agua marina), en ejemplares maduros machos y hembras. Las enzimas digestivas mostraron un grado de actividad notablemente mayor en el hepatopán-creas de los machos que en el de las hembras, con excepción de la lipasa. En las hembras, la actividad de la amilasa, la pepsina y la tripsina comenzó a reducirse notablemente cuando la salinidad alcanzó las 28 ppt, mientras que la actividad de la celulasa descendió cuando se alcanzaron las 35 ppt; en el caso de los machos, se observó un descenso muy pronunciado de la actividad enzimática digestiva a partir de las 21 ppt, aunque la actividad aumentó a las 14 ppt. La menor actividad enzimát...

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Section: Discussionsupporting

confidence: 90%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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“…Indeed the apparent lack of β-glucohydrolyase in B. latro, as suggested by activity staining, suggests that this species is incapable of hydrolysing cellulose to glucose. The carnivorous mud crab, Scylla serrata also possesses endo-β-1,4-glucanase activities within its midgut gland (Pavasovic et al 2004). like B. latro, this species would not derive a significant amount of energy from cellulose digestion.…”

Section: Discussionmentioning

confidence: 99%

“…although phenotypic plasticity of digestive enzymes remains to be demonstrated in land crabs, it has been observed in other decapod crustaceans and insects (Locusta migratoria). activities of α-amylase in the midgut gland of mud crabs, Scylla serrata increase when the crabs are fed diets increasing in starch (Pavasovic et al 2004). Similarly, activities of the digestive enzymes, α-amylase, endo-β-1,4-glucanase and protease within the crayfish, C. quadricarinatus, are positively correlated to the protein and carbohydrate content of the diet (Pavasovic et al 2007a, b).…”

Section: Discussionmentioning

confidence: 99%

Digestive enzymes of two brachyuran and two anomuran land crabs from Christmas Island, Indian Ocean

et al. 2014

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higher endo-β-1,4-glucanase, lichenase and laminarinase activities compared to that of the other species. Thus, C. perlatus may be efficient at digestion of cellulose and hemicellulose within plant material. Zymography indicated that the majority of protease, lipase, phosphatase, amylase, endo-β-1,4-glucanase, β-glucohydrolase and N-acetyl-β-d-glucosaminidase isozymes were common to all species, and hence were inherited from a common aquatic ancestor. Differences were observed for the phosphatase, lipase and endo-β-1,4-glucanase isozymes. These differences are discussed in relation to phylogeny and possible evolution to cope with the adoption of a terrestrial diet.

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“…In contrast, in the white shrimp Penaeus vannamei dietary protein level did not influence total protease activity, although modulation of trypsin-and chymotrypsin-like activities has been documented (Muhlia-Almazan et al, 2003). Moreover, no significant difference was observed in protease activity of Litopenaeus vannamei (Rivas-Vega et al, 2006) and Scylla serrata subjected to different dietary treatments (Pavasovic et al, 2004). In Cherax quadricarinatus the digestive enzyme secretion is modified by the diet composition, such as the presence of animal-or plant-derived ingredients (Lopez-Lopez et al, 2005;Pavasovic et al, 2007a).…”

Section: Binder Effect On Digestive Enzymesmentioning

confidence: 91%

Development of Biopolymers as Binders for Feed for Farmed Aquatic Organisms

Paolucci¹,

Fabbrocini²,

Volpe³

et al. 2012

Aquaculture

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Effect of pH, temperature and diet on digestive enzyme profiles in the mud crab, Scylla serrata

Cited by 84 publications

References 21 publications

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

Digestive enzymes of two brachyuran and two anomuran land crabs from Christmas Island, Indian Ocean

Development of Biopolymers as Binders for Feed for Farmed Aquatic Organisms

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