Effect of pH on the reaction of tris(2,2′-bipyridyl)ruthenium(III) with amino-acids: Implications for their detection

Brune, Stephen N.

doi:10.1016/0039-9140(91)80080-j

Cited by 115 publications

(56 citation statements)

References 15 publications

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“…17 The NAR response was increased at much higher pHs. Burune and Bobbitt reported that the obseved CL for amino-acids is dependent upon the pHs 13 , and that the CL intensity was also affected by the sorts of amines. Although the exact reaction mechanism in this method is not clear at present, the difference in the CL intensity between YOH and NAR may be presumed to be caused by some variables, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…Recently a chemiluminescence (CL) detection system using tris(2,2′-bipyridyl)ruthenium(II), Ru(bpy) 3 2+ , as a chemiluminescent reagent has been shown to be a sensitive and selective detection technique in high-performance liquid chromatography (HPLC) and flow injection analysis for the determination of aliphatic and alicyclic tertiary amines [9][10][11][12][13] , oxalate [14][15][16] and indoles. 17 For the preparation of unstable Ru(bpy) 3 3+ from stable Ru(bpy) 3 2+ , three methods were reported using a batch electrochemical reactor 13 , an on-line electrochemical reactor 17 and an electrogenerated CL flow cell.…”

mentioning

confidence: 99%

“…17 For the preparation of unstable Ru(bpy) 3 3+ from stable Ru(bpy) 3 2+ , three methods were reported using a batch electrochemical reactor 13 , an on-line electrochemical reactor 17 and an electrogenerated CL flow cell. More recently, Yamazaki et al showed that an on-line photochemical reactor could be applicable for the purpose of preparation.…”

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confidence: 99%

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High-Performance Liquid Chromatographic Determination of Yohimbine in Serum by Chemiluminescence Detection

et al. 1998

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An ion-pair high-performance liquid chromatography with chemiluminescence detection using tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)3 2+ ] was developed for the determination of yohimbine (YOH) in human serum. A sample solution, containing narceine as an internal standard, was treated with a Bond-Elut Certify disposable column, and the purified fraction was separated on a TSKgel ODS-80Ts column. The recovery of YOH at 50 ng/ml was 97.2±4.4% (mean±SD, n=5). The detection limit was calculated to be 30 ng/ml of YOH in serum (S/N=3). The method was successfully applied to drug monitoring of the YOH level in the serum of healthy volunteers.

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Section: Resultsmentioning

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High-Performance Liquid Chromatographic Determination of Yohimbine in Serum by Chemiluminescence Detection

et al. 1998

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“…[2][3][4][5] Over the last two decades, Ru(bpy) 3 2+ ECL has attracted much attention as a powerful an analytical method due to its inherent sensitivity, selectivity, and wide dynamic range for a variety of analytes [3][4][5] such as oxalate, 6 alkylamines, 7 amino acids, 8 NADH, 9 DNA, 10 and a number of pharmaceutical compounds. [11][12][13][14][15] In the Ru(bpy)3 2+ ECL process, the starting material Ru(bpy) 3 2+ is regenerated and thus can be permanently reused in theory when it is immobilized on an electrode surface.…”

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confidence: 99%

Electrogenerated Chemiluminescence Sensor Based on Tris(2,2'-bipyridyl) ruthenium(II) Immobilized in the Composite Film of Multi-walled Carbon Nanotube/Sol-gel Zinc oxide/Nafion

Choi

Kang

Choi

et al. 2009

Bulletin of the Korean Chemical Society

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A composite film of multi-walled carbon nanotube (MWCNT)/sol-gel-derived zinc oxide(ZnO)/Nafion has been utilized as an efficient immobilization matrix for the construction of a highly sensitive and stable tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)3 2+ ) electrogenerated chemiluminescence (ECL) sensor. The electrochemical and ECL behaviors of Ru(bpy)3 2+ ion-exchanged into the composite film were strongly dependent upon the sol-gel preparation condition, the amount of MWCNT incorporated into the ZnO/Nafion composite film, and the buffer solution pH. The synergistic effect of MWCNTs and ZnO in the composite films increased not only the sensitivity but also the long-term stability of the ECL sensor. The present ECL sensor based on the MWCNT/ZnO/Nafion gave a linear response (R 2 = 0.999) for tripropylamine concentration from 500 nM to 1.0 mM with a remarkable detection limit (S/N = 3) of 15 nM. The present ECL sensor showed outstanding long-term stability (94% initial signal retained for 5 weeks). Since the present ECL sensor exhibits large response towards NADH, it could be applied as a transduction platform for the ECL biosensor in which the NADH is produced from the dehydrogenase-based enzymatic reaction in the presence of NAD + cofactor.

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“…The optimum signal-tonoise ratio could be obtained when the buffer pH was at slightly basic. 49 A pH study was carried out in our experiments to determine the effect of the buffer pH on the Ru(bpy)3 2+ ECL intensity of AT and ME over a wide range of pH values (from 5 to 10, 0.5 as an interval). As can be seen from Fig.…”

Section: Optimization Of Detection Conditions In the Ecl Cellmentioning

confidence: 99%

Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

et al. 2007

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Atenolol (AT) and metoprolol (ME) are antihypertensive drugs that belong to the family of selective β(1)-adrenergic receptor antagonists (β-blockers), which are widely used in the treatment of hypertension, angina pectoris, cardiac arrhythmias and myocardial infarction. 1 Due to their sedative effect, they are also used as doping agents in sports. An overdose of β-blockers may lead to bradycardia, hypotension, aggravation of cardiac failure, bronchospasm, hypoglycemia, and fatigue.2 Therefore, it is necessary to develop a sensitive and simple analytical method to determine β-blockers in many fields, including doping control, forensic analysis, toxicology and pharmacokinetic study.As a consequence of the widespread use of AT and ME, several analytical techniques have been developed for their determination in recent years, including spectrophotometry [3][4][5] and differential pulse voltammetry. 6,7 However, the sensitivity of these methods was limited. The most widely used were chromatographic techniques, such as gas chromatography (GC), 8,9 high-performance liquid chromatography (HPLC) [10][11][12][13][14][15][16][17] and urtra-performance liquid chromatography (UPLC). 18 Among them, expensive columns and costly apparatus were needed, and the analysis time was relatively long.The capillary electrophoresis (CE) technique has been developed for over 20 years, and has become a mature and wellestablished analytical tool. CE is characterized as a powerful separation technique because of its high efficiency, relatively short analysis time, and low sample and reagent consumption. As an alternative and complementary technique to HPLC for routine analysis, CE-UV has been used for AT and ME analysis. 19-22Because of the small sample volume being assayed, this method has encountered the problem of poor sensitivity, which has limited its application in practice. He et al. 23 developed an on-line ion-exchange preconcentration method for improving the detection sensitivity of CE-UV. The proposed method enhanced the detection sensitivity of CE-UV by about 5000 times for metoprolol compared with the normal electrokinetic injection.However, the preparation of a preconcentration column was complex for a general laboratory. Thus, it is very important to develop a simple and sensitive method to detect AT and ME.Electrochemiluminescence (also called electrogenerated chemiluminescence) is a means of converting electrical energy into radiative energy. 24 It has been proved to be a powerful analytical tool that combines the simplicity of electrochemistry and the inherent sensitivity and the wide linear range of the chemiluminescence method. 25Among the ECL systems, tris(2,2′-bipyridyl)ruthenium(II) Ru(bpy)3 2+ -based ECL is the most valuable in fundamental studies and applications because of its stability and capability of undergoing ECL at room temperature in aqueous solutions and its regenerating during the ECL process. 26Since the first report of Ru(bpy)3 2+ ECL in 1972, 27 Ru(bpy)3 2+ ECL as a sensitive detection method has been...

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Effect of pH on the reaction of tris(2,2′-bipyridyl)ruthenium(III) with amino-acids: Implications for their detection

Cited by 115 publications

References 15 publications

High-Performance Liquid Chromatographic Determination of Yohimbine in Serum by Chemiluminescence Detection

High-Performance Liquid Chromatographic Determination of Yohimbine in Serum by Chemiluminescence Detection

Electrogenerated Chemiluminescence Sensor Based on Tris(2,2'-bipyridyl) ruthenium(II) Immobilized in the Composite Film of Multi-walled Carbon Nanotube/Sol-gel Zinc oxide/Nafion

Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection

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